Mouse anti Cat CD4 antibody, clone vpg34 recognizes the feline homolog of the human CD4 antigen. CD4 is not expressed on feline monocytes.
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Immunoaffinity purified feline CD4.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c were fused with cells of the NS0 mouse myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD4 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 lymphocytes in 100ul
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD4 antibody
Willett, B.J., and Callanan, J.J. (1995) The expression of leucocyte differentiation antigens in the feline immune system., p 3-15. In B.J. Willett and O.J. Jarrett (eds). Feline Immunology and Immunodeficiency.
Oxford University Press, Oxford.
Callanan, J.J., Racz, P., Thompson, H. and Jarrett, O. P. (1993) Morphologic characterization of the lymph node changes in feline immunodeficiency virus infection as an animal model of AIDS.
In P. Racz, N. L. Letvin, and J.C. Gluckman (eds)., Animal models of HIV and other retroviral infections. S. Karger, Basel, Switzerland. pp 115-136.
Willett, B.J. et al. (1994) The generation of monoclonal antibodies recognising novel epitopes by immunisation with solid matrix antigen-antibody complexes reveals a polymorphic determinant on feline CD4.
J Immunol Methods. 176 (2): 213-20.
Campbell, D.J. et al. (2004) Age-related differences in parameters of feline immune status.
Vet Immunol Immunopathol. 100: 73-80.
Campbell, D.J. et al. (2004) Insulin-like growth factor-I (IGF-I) and its association with lymphocyte homeostasis in the ageing cat.
Mech Ageing Dev. 125: 497-505.
Veir, J.K. et al. (2007) Effect of supplementation with Enterococcus faecium (SF68) on immune functions in
Vet Ther. 8: 229-38.
Freer, G. et al. (2008) Immunotherapy with internally inactivated virus loaded dendritic cells boosts cellular immunity but does not affect feline immunodeficiency virus infection course.
Retrovirology. 5: 33.
Pistello, M. et al. (2010) Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus.
J Virol. 84: 3845-56.
Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
J Virol. 81: 9665-79.
Reinero, C.R. et al. (2008) Adjuvanted rush immunotherapy using CpG oligodeoxynucleotides in experimental feline allergic asthma.
Vet Immunol Immunopathol. 121: 241-50.
Carreño, A.D. et al. (2008) Loss of naïve (CD45RA+) CD4+ lymphocytes during pediatric infection with feline immunodeficiency virus.
Vet Immunol Immunopathol. 121: 161-8.
Flynn, J.N. et al. (2002) Longitudinal analysis of feline leukemia virus-specific cytotoxic T lymphocytes: correlation with recovery from infection.
J Virol. 76: 2306-15.
Hosie, M.J. et al. (2000) Vaccination with inactivated virus but not viral DNA reduces virus load following challenge with a heterologous and virulent isolate of feline immunodeficiency virus.
J Virol. 74: 9403-11.
Hosie, M.J. et al. (2002) Evolution of replication efficiency following infection with a molecularly cloned feline immunodeficiency virus of low virulence.
J Virol. 76: 6062-72.
Kraase, M. et al. (2010) Feline immunodeficiency virus env gene evolution in experimentally infected cats.
Vet Immunol Immunopathol. 134: 96-106.
Milner, R.J. et al. (2004) Suppurative rhinitis associated with Haemophilus species infection in a cat.
J S Afr Vet Assoc. 75: 103-7.
Novacco, M. et al. (2012) Protection from reinfection in "Candidatus Mycoplasma turicensis"-infected cats and characterization of the immune response.
Vet Res. 43: 82.
Reche, A.Jr. et al. (2010) Cutaneous mycoflora and CD4:CD8 ratio of cats infected with feline immunodeficiency virus.
J Feline Med Surg. 12: 355-8.
Willett, B.J. et al. (2013) Selective expansion of viral variants following experimental transmission of a reconstituted feline immunodeficiency virus quasispecies.
PLoS One. 8: e54871.
Webb, C. et al. (2006) Use of flow cytometry and monochlorobimane to quantitate intracellular glutathione concentrations in feline leukocytes.
Vet Immunol Immunopathol. 112 (3-4): 129-40.
Webb, C. et al. (2008) Oxidative stress during acute FIV infection in cats.
Vet Immunol Immunopathol. 122 (1-2): 16-24.
Avery, P.R. et al. (2007) Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures.
Vet Immunol Immunopathol. 117 (3-4): 222-35.
McLuckie, A.J. et al. (2016) Detection of Felis catus gammaherpesvirus 1 (FcaGHV1) in peripheral blood B- and T-lymphocytes in asymptomatic, naturally-infected domestic cats.
Virology. 497: 211-6.
Veir, J.K. et al. (2006) Evaluation of a novel immunotherapy for treatment of chronic rhinitis in cats.
J Feline Med Surg. 8 (6): 400-11.
Sugiarto, S. et al. (2016) Passive immunization does not provide protection against experimental infection with Mycoplasma haemofelis.
Vet Res. 47 (1): 79.
Baumann J et al. (2015) Lack of cross-protection against Mycoplasma haemofelis infection and signs of enhancement in "Candidatus Mycoplasma turicensis"-recovered cats.
Vet Res. 46: 104.
MirandaL, H.M. et al. (2018) Co-infection with feline retrovirus is related to changes in immunological parameters of cats with sporotrichosis.
PLoS One. 13 (11): e0207644.
Maeta, N. et al. (2019) Lymphokine-activated killer cell transplantation after anti-cancer treatment in two aged cats.
Open Vet J. 9 (2): 147-50.
Sieg, M. et al. (2019) A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood.
Viruses. 11 (2) Feb 09 [Epub ahead of print].
de Oliveira Matheus, L.F. et al. (2021) Effects of Saccharomyces cerevisiae. cell wall addition on feed digestibility, fecal fermentation and microbiota and immunological parameters in adult cats.
BMC Vet Res. 17 (1): 351.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra