CD4 antibody | vpg34
FITC conjugated Mouse anti Feline CD4 antibody, clone vpg34 (MCA1346F) used for the identification of CD4+ lymphocytes in peripheral blood smears by immunocytochemistry.
Image caption:
Lymphocyte subsets after M. haemofelis exposure in the ten SPF cats. The five cats in group A had undergone previous “Cand. M. turicensis” infection and the five cats in group B were naïve control cats. The M. haemofelis exposure took place on day 0. A) T cells (CD5+ MHCII+) counts, B) CD4+ T cell counts, C) CD8+ T cell counts, D) CD4+/CD8+ ratio, E) CD4+CD25+ activated T cells counts and F) B cell (CD45R/B220+) counts. No significant differences between groups A and B were observed on day 0; significant differences between the two groups observed thereafter are indicated with an asterisk (pMWU <0.05). Significant differences over time (pF<0.05 and pD<0.05) and the corresponding significantly decreased or increased values are indicated with an arrow (black solid for group A and gray open for group B). In detail, increased T cell counts were observed in group A on days 2, 6 and 62 compared with day 83 and in group B on day 9 and 20 compared with day 83 (A). Decreased CD8+ T lymphocyte counts were observed in group A on day 20 compared with days 57 and 83 (C). In the CD4+/CD8+ ratio, in group A, significantly lower values were observed on day 48 compared to days 0 and 41, whereas in group B, higher counts were observed on day 0 compared to days 9 and 83 and on and 41 compared to day 9 (D). CD4+CD25+ cell counts varied significantly over time in cats in group B, with lower values on days 2, 9 and 27 compared with day 6 (E). B cells varied significantly over time in cats in group A, with transiently decreased values on days 20, 27, 34 and 41 compared with days 9, 62, 76 and 83 (F). No values were available for CD4+CD25+ on day 83.
From: Baumann J, Novacco M, Willi B, Riond B, Meli ML, Boretti FS, Hofmann-Lehmann R.
Lack of cross-protection against Mycoplasma haemofelis infection and signs of enhancement in "Candidatus Mycoplasma turicensis"-recovered cats.
Vet Res. 2015 Sep 24;46:104.
FITC conjugated Mouse anti Feline CD4 antibody, clone vpg34 (MCA1346F) used to evaluate circulating CD4 expressing T lymphocyte prevelence in Feline immunodeficiency virus infected cat blood by flow cytometry.
Image caption:
Plasma viremia (a), proviral load in the PBMCs (b), and circulating CD4+ T lymphocyte percentages (c) of the study cats at the times indicated relative to the first FIV-MDC inoculum. CD4+ T cells were monitored by flow cytometry in peripheral blood by direct staining with anti-feline CD4-PE (clone vpg34, AbD Serotec, Raleigh, NC) for 30 min as previously described (8). Symbols represent individual animals. Arrows indicate the times of FIV-MDC inoculation.
From: Freer G, Matteucci D, Mazzetti P, Tarabella F, Ricci E, Bozzacco L, Merico A, Pistello M, Ceccherini-Nelli L, Bendinelli M.
Immunotherapy with internally inactivated virus loaded dendritic cells boosts cellular immunity but does not affect feline immunodeficiency virus infection course.
Retrovirology. 2008 Apr 17;5:33.
Mouse anti Feline CD4 antiody, clone vpg34 (MCA1346F) used for the evaluation of CD4 expressing T cell prevelence in peripheral blood by flow cytometry.
Image caption:
Immunological alterations following FIV infection.
Cats in Group 1 were challenged with clonal GL8 (variant B32), cats in group 2 were challenged with the reconstituted quasispecies challenge (B14, B19, B28, B30, B31 and B32) and control cats remained unchallenged. Percentages of lymphocytes expressing (A) CD4 and (B) CD8 were measured by flow cytometry and are shown as group means +/− SE. (C) CD4:CD8 ratios were calculated from the percentage of CD4 and CD8 positive lymphocytes and are displayed as group means +/− SE. p-values for statistically significant differences between infected and control groups are shown (Student's t-test).
From: Willett BJ, Kraase M, Logan N, McMonagle E, Varela M, Hosie MJ (2013)
Selective Expansion of Viral Variants following Experimental Transmission of a Reconstituted Feline Immunodeficiency Virus Quasispecies.
PLoS ONE 8(1): e54871.
Mouse anti Feline CD4 antibody, clone vpg34 (MCA1346F) used to assess changes in CD4 expressing T lymphocytes in cat blood following Mycoplasma heamofelis infection, by flow cytometry.
Image caption:
CD4+ , CD8+ and CD5+ T lymphocytes, B lymphocytes and activated CD4+ T lymphocytes during the course of Mhf infection. Mean (±SD) cell counts of the CD4+ (A), CD8+ (B) and CD5+ T lymphocytes (C), CD4+/CD8+ ratio (D), B lymphocytes (CD45R/B220+, E) and activated CD4+ T lymphocytes (CD4+CD25+, F) at day 0 and during the 100-day pi period in group A (black dots, solid line) and group B (open triangles, dashed line). Cats were subcutaneously inoculated with Mhf at day 0. The onset of bacteremia in group A and B (day 7 pi, both groups) is indicated with a triangle. Boxes represent the time of peak bacteremia in group A (grey box) and B (dotted box). Statistically significant differences between group A and B are indicated with asterisks (pMWU<0.05). A CD4+ T lymphocyte counts changed significantly over time in group A (pF = 0.0012, lower at day 21 pi compared with days 3, 70 and 98 pi, pD<0.05). B CD8+ T lymphocyte counts changed significantly over time in group A (pF = 0.0002; lower at day 21 pi compared with days 3, 70 and 98 pi, pD<0.05). C CD5+ T lymphocyte counts changed significant over time in group A (pF<0.0002, lower at day 21 pi compared with days 3, 70 and 98 pi, pD<0.05). D CD4+/CD8+ ratio changed significantly over time in group A (pF = 0.0002, lower at days 14 and 21 pi compared with day 0 and day 98 pi, pD<0.05). E B lymphocyte counts changed significantly over time in group A (pF = 0.0103, lower at day 21 pi compared with day 3 pi, pD<0.05). F Activated CD4+ T lymphocyte counts changed significantly over time in group A (pF<0.0001, higher at days 3, 14 and 21 pi when compared with days 42, 56, 70 and 98 pi, pD<0.05) and group B (pF = 0.0249, no significance in the post test).
From: Sugiarto S, Spiri AM, Riond B, Novacco M, Oestmann A, de Miranda LH, Meli ML, Boretti FS, Hofmann-Lehmann R, Willi B.
Passive immunization does not provide protection against experimental infection with Mycoplasma haemofelis.
Vet Res. 2016 Aug 5;47(1):79.
Mouse anti Cat CD4 antibody, clone vpg34 recognizes the feline homolog of the human CD4 antigen. CD4 is not expressed on feline monocytes.
Product Details
- Target Species
- Cat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Immunoaffinity purified feline CD4.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c were fused with cells of the NS0 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of CD4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 lymphocytes in 100ul
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for CD4 antibody
-
Willett, B.J. et al. (1994) The generation of monoclonal antibodies recognising novel epitopes by immunisation with solid matrix antigen-antibody complexes reveals a polymorphic determinant on feline CD4.
J Immunol Methods. 176 (2): 213-20. -
Campbell, D.J. et al. (2004) Age-related differences in parameters of feline immune status.
Vet Immunol Immunopathol. 100: 73-80. -
Campbell, D.J. et al. (2004) Insulin-like growth factor-I (IGF-I) and its association with lymphocyte homeostasis in the ageing cat.
Mech Ageing Dev. 125: 497-505. -
Veir, J.K. et al. (2007) Effect of supplementation with Enterococcus faecium (SF68) on immune functions in
cats.
Vet Ther. 8: 229-38. -
Freer, G. et al. (2008) Immunotherapy with internally inactivated virus loaded dendritic cells boosts cellular immunity but does not affect feline immunodeficiency virus infection course.
Retrovirology. 5: 33. -
Pistello, M. et al. (2010) Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus.
J Virol. 84: 3845-56. -
Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
J Virol. 81: 9665-79. -
Reinero, C.R. et al. (2008) Adjuvanted rush immunotherapy using CpG oligodeoxynucleotides in experimental feline allergic asthma.
Vet Immunol Immunopathol. 121: 241-50. -
Carreño, A.D. et al. (2008) Loss of naïve (CD45RA+) CD4+ lymphocytes during pediatric infection with feline immunodeficiency virus.
Vet Immunol Immunopathol. 121: 161-8. -
Flynn, J.N. et al. (2002) Longitudinal analysis of feline leukemia virus-specific cytotoxic T lymphocytes: correlation with recovery from infection.
J Virol. 76: 2306-15. -
Hosie, M.J. et al. (2000) Vaccination with inactivated virus but not viral DNA reduces virus load following challenge with a heterologous and virulent isolate of feline immunodeficiency virus.
J Virol. 74: 9403-11. -
Hosie, M.J. et al. (2002) Evolution of replication efficiency following infection with a molecularly cloned feline immunodeficiency virus of low virulence.
J Virol. 76: 6062-72. -
Kraase, M. et al. (2010) Feline immunodeficiency virus env gene evolution in experimentally infected cats.
Vet Immunol Immunopathol. 134: 96-106. -
Milner, R.J. et al. (2004) Suppurative rhinitis associated with Haemophilus species infection in a cat.
J S Afr Vet Assoc. 75: 103-7. -
Novacco, M. et al. (2012) Protection from reinfection in "Candidatus Mycoplasma turicensis"-infected cats and characterization of the immune response.
Vet Res. 43: 82. -
Reche, A.Jr. et al. (2010) Cutaneous mycoflora and CD4:CD8 ratio of cats infected with feline immunodeficiency virus.
J Feline Med Surg. 12: 355-8. -
Willett, B.J. et al. (2013) Selective expansion of viral variants following experimental transmission of a reconstituted feline immunodeficiency virus quasispecies.
PLoS One. 8: e54871. -
Webb, C. et al. (2006) Use of flow cytometry and monochlorobimane to quantitate intracellular glutathione concentrations in feline leukocytes.
Vet Immunol Immunopathol. 112 (3-4): 129-40. -
Webb, C. et al. (2008) Oxidative stress during acute FIV infection in cats.
Vet Immunol Immunopathol. 122 (1-2): 16-24. -
Avery, P.R. et al. (2007) Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures.
Vet Immunol Immunopathol. 117 (3-4): 222-35. -
McLuckie, A.J. et al. (2016) Detection of Felis catus gammaherpesvirus 1 (FcaGHV1) in peripheral blood B- and T-lymphocytes in asymptomatic, naturally-infected domestic cats.
Virology. 497: 211-6. -
Veir, J.K. et al. (2006) Evaluation of a novel immunotherapy for treatment of chronic rhinitis in cats.
J Feline Med Surg. 8 (6): 400-11. -
Sugiarto, S. et al. (2016) Passive immunization does not provide protection against experimental infection with Mycoplasma haemofelis.
Vet Res. 47 (1): 79. -
Baumann J et al. (2015) Lack of cross-protection against Mycoplasma haemofelis infection and signs of enhancement in "Candidatus Mycoplasma turicensis"-recovered cats.
Vet Res. 46: 104.
