CD4 antibody | vpg34

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Mouse anti CAT CD4:FITC

Product Type
Monoclonal Antibody
Clone
vpg34
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1346F F 0.1 mg
Mouse anti Cat CD4 antibody, clone vpg34 recognizes the feline homolog of the human CD4 antigen. CD4 is not expressed on feline monocytes.

Product Details

Target Species
Cat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Immunoaffinity purified feline CD4.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Fusion Partners
Spleen cells from immunised BALB/c were fused with cells of the NS0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 lymphocytes in 100ul

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F 0.1 mg F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD4 antibody

  1. Willett, B.J. et al. (1994) The generation of monoclonal antibodies recognising novel epitopes by immunisation with solid matrix antigen-antibody complexes reveals a polymorphic determinant on feline CD4.
    J Immunol Methods. 176 (2): 213-20.
  2. Campbell, D.J. et al. (2004) Age-related differences in parameters of feline immune status.
    Vet Immunol Immunopathol. 100: 73-80.
  3. Campbell, D.J. et al. (2004) Insulin-like growth factor-I (IGF-I) and its association with lymphocyte homeostasis in the ageing cat.
    Mech Ageing Dev. 125: 497-505.
  4. Veir, J.K. et al. (2007) Effect of supplementation with Enterococcus faecium (SF68) on immune functions in cats.
    Vet Ther. 8: 229-38.
  5. Freer, G. et al. (2008) Immunotherapy with internally inactivated virus loaded dendritic cells boosts cellular immunity but does not affect feline immunodeficiency virus infection course.
    Retrovirology. 5: 33.
  6. Pistello, M. et al. (2010) Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus.
    J Virol. 84: 3845-56.
  7. Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
    J Virol. 81: 9665-79.
  8. Reinero, C.R. et al. (2008) Adjuvanted rush immunotherapy using CpG oligodeoxynucleotides in experimental feline allergic asthma.
    Vet Immunol Immunopathol. 121: 241-50.
  9. Carreño, A.D. et al. (2008) Loss of naïve (CD45RA+) CD4+ lymphocytes during pediatric infection with feline immunodeficiency virus.
    Vet Immunol Immunopathol. 121: 161-8.
  10. Flynn, J.N. et al. (2002) Longitudinal analysis of feline leukemia virus-specific cytotoxic T lymphocytes: correlation with recovery from infection.
    J Virol. 76: 2306-15.
  11. Hosie, M.J. et al. (2000) Vaccination with inactivated virus but not viral DNA reduces virus load following challenge with a heterologous and virulent isolate of feline immunodeficiency virus.
    J Virol. 74: 9403-11.
  12. Hosie, M.J. et al. (2002) Evolution of replication efficiency following infection with a molecularly cloned feline immunodeficiency virus of low virulence.
    J Virol. 76: 6062-72.
  13. Kraase, M. et al. (2010) Feline immunodeficiency virus env gene evolution in experimentally infected cats.
    Vet Immunol Immunopathol. 134: 96-106.
  14. Milner, R.J. et al. (2004) Suppurative rhinitis associated with Haemophilus species infection in a cat.
    J S Afr Vet Assoc. 75: 103-7.
  15. Novacco, M. et al. (2012) Protection from reinfection in "Candidatus Mycoplasma turicensis"-infected cats and characterization of the immune response.
    Vet Res. 43: 82.
  16. Reche, A.Jr. et al. (2010) Cutaneous mycoflora and CD4:CD8 ratio of cats infected with feline immunodeficiency virus.
    J Feline Med Surg. 12: 355-8.
  17. Willett, B.J. et al. (2013) Selective expansion of viral variants following experimental transmission of a reconstituted feline immunodeficiency virus quasispecies.
    PLoS One. 8: e54871.
  18. Webb, C. et al. (2006) Use of flow cytometry and monochlorobimane to quantitate intracellular glutathione concentrations in feline leukocytes.
    Vet Immunol Immunopathol. 112 (3-4): 129-40.
  19. Webb, C. et al. (2008) Oxidative stress during acute FIV infection in cats.
    Vet Immunol Immunopathol. 122 (1-2): 16-24.
  20. Avery, P.R. et al. (2007) Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures.
    Vet Immunol Immunopathol. 117 (3-4): 222-35.
  21. McLuckie, A.J. et al. (2016) Detection of Felis catus gammaherpesvirus 1 (FcaGHV1) in peripheral blood B- and T-lymphocytes in asymptomatic, naturally-infected domestic cats.
    Virology. 497: 211-6.
  22. Veir, J.K. et al. (2006) Evaluation of a novel immunotherapy for treatment of chronic rhinitis in cats.
    J Feline Med Surg. 8 (6): 400-11.
  23. Sugiarto, S. et al. (2016) Passive immunization does not provide protection against experimental infection with Mycoplasma haemofelis.
    Vet Res. 47 (1): 79.
  24. Baumann J et al. (2015) Lack of cross-protection against Mycoplasma haemofelis infection and signs of enhancement in "Candidatus Mycoplasma turicensis"-recovered cats.
    Vet Res. 46: 104.