CD4 antibody | vpg34
Mouse anti Cat CD4 antibody, clone vpg34 recognizes the feline homolog of the human CD4 antigen. CD4 is not expressed on feline monocytes.
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin
- Immunoaffinity purified feline CD4.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c were fused with cells of the NS0 mouse myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- For research purposes only
Applications of CD4 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 lymphocytes in 100ul
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Mouse IgG1 Negative Control:FITC||MCA1209F||0.1 mg||F|
|Mouse IgG1 Negative Control:FITC||MCA928F||100 Tests||F|
Product Specific References
References for CD4 antibody
Willett, B.J. et al. (1994) The generation of monoclonal antibodies recognising novel epitopes by immunisation with solid matrix antigen-antibody complexes reveals a polymorphic determinant on feline CD4.
J Immunol Methods. 176 (2): 213-20.
Campbell, D.J. et al. (2004) Age-related differences in parameters of feline immune status.
Vet Immunol Immunopathol. 100: 73-80.
Campbell, D.J. et al. (2004) Insulin-like growth factor-I (IGF-I) and its association with lymphocyte homeostasis in the ageing cat.
Mech Ageing Dev. 125: 497-505.
Veir, J.K. et al. (2007) Effect of supplementation with Enterococcus faecium (SF68) on immune functions in
Vet Ther. 8: 229-38.
Freer, G. et al. (2008) Immunotherapy with internally inactivated virus loaded dendritic cells boosts cellular immunity but does not affect feline immunodeficiency virus infection course.
Retrovirology. 5: 33.
Pistello, M. et al. (2010) Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus.
J Virol. 84: 3845-56.
Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
J Virol. 81: 9665-79.
Reinero, C.R. et al. (2008) Adjuvanted rush immunotherapy using CpG oligodeoxynucleotides in experimental feline allergic asthma.
Vet Immunol Immunopathol. 121: 241-50.
Carreño, A.D. et al. (2008) Loss of naïve (CD45RA+) CD4+ lymphocytes during pediatric infection with feline immunodeficiency virus.
Vet Immunol Immunopathol. 121: 161-8.
Flynn, J.N. et al. (2002) Longitudinal analysis of feline leukemia virus-specific cytotoxic T lymphocytes: correlation with recovery from infection.
J Virol. 76: 2306-15.
Hosie, M.J. et al. (2000) Vaccination with inactivated virus but not viral DNA reduces virus load following challenge with a heterologous and virulent isolate of feline immunodeficiency virus.
J Virol. 74: 9403-11.
Hosie, M.J. et al. (2002) Evolution of replication efficiency following infection with a molecularly cloned feline immunodeficiency virus of low virulence.
J Virol. 76: 6062-72.
Kraase, M. et al. (2010) Feline immunodeficiency virus env gene evolution in experimentally infected cats.
Vet Immunol Immunopathol. 134: 96-106.
Milner, R.J. et al. (2004) Suppurative rhinitis associated with Haemophilus species infection in a cat.
J S Afr Vet Assoc. 75: 103-7.
Novacco, M. et al. (2012) Protection from reinfection in "Candidatus Mycoplasma turicensis"-infected cats and characterization of the immune response.
Vet Res. 43: 82.
Reche, A.Jr. et al. (2010) Cutaneous mycoflora and CD4:CD8 ratio of cats infected with feline immunodeficiency virus.
J Feline Med Surg. 12: 355-8.
Willett, B.J. et al. (2013) Selective expansion of viral variants following experimental transmission of a reconstituted feline immunodeficiency virus quasispecies.
PLoS One. 8: e54871.
Webb, C. et al. (2006) Use of flow cytometry and monochlorobimane to quantitate intracellular glutathione concentrations in feline leukocytes.
Vet Immunol Immunopathol. 112 (3-4): 129-40.
Webb, C. et al. (2008) Oxidative stress during acute FIV infection in cats.
Vet Immunol Immunopathol. 122 (1-2): 16-24.
Avery, P.R. et al. (2007) Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures.
Vet Immunol Immunopathol. 117 (3-4): 222-35.
McLuckie, A.J. et al. (2016) Detection of Felis catus gammaherpesvirus 1 (FcaGHV1) in peripheral blood B- and T-lymphocytes in asymptomatic, naturally-infected domestic cats.
Virology. 497: 211-6.
Veir, J.K. et al. (2006) Evaluation of a novel immunotherapy for treatment of chronic rhinitis in cats.
J Feline Med Surg. 8 (6): 400-11.
Sugiarto, S. et al. (2016) Passive immunization does not provide protection against experimental infection with Mycoplasma haemofelis.
Vet Res. 47 (1): 79.
Baumann J et al. (2015) Lack of cross-protection against Mycoplasma haemofelis infection and signs of enhancement in "Candidatus Mycoplasma turicensis"-recovered cats.
Vet Res. 46: 104.