Canakinumab antibody | AbD54116




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E ResetHuman anti Canakinumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD54116
- Isotype
- Fab antibody
- Specificity
- Canakinumab
Human Anti-Canakinumab Antibody, clone AbD54116ad is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug canakinumab, and its biosimilar products. It does not recognize the drug target, the human interleukin-1 beta (IL-1β), nor canakinumab in complex with IL-1β. This antibody can be used to measure free canakinumab levels in serum from patients. A pair of anti-canakinumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD54116ad is recommended as a capture antibody, paired with Human Anti-Canakinumab Antibody, clone AbD54331 (TZA064) as the detection antibody. TZA063 is a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog. Canakinumab (trade name Ilaris) is a fully humanized monoclonal IgG1/kappa antibody with allotype G1m3. It binds to interleukin-1 beta (IL-1β) to neutralize up-regulated 1β signaling, resulting in suppression of inflammation in patients with disorders of autoimmune origin. Canakinumab is indicated for treatment of Cryopyrin-Associated Periodic Syndromes (CAPS), three rare and serious auto-inflammatory diseases, Still’s disease, and gouty arthritis. |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Canakinumab
- Affinity
- The monovalent intrinsic affinity of AbD54116ad was measured as KD = 9.6 nM by real time, label free molecular interaction analysis on immobilized canakinumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Ilaris is a trademark of Novartis AG. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
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ELISA | ![]() |
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD54116ad can be used in indirect ELISA to detect canakinumab. It can also be used as a capture antibody to develop a pharmacokinetic (PK) bridging ELISA for canakinumab, together with TZA064 or TZA064P as the detection reagent.
Protocol: PK bridging ELISA
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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Description | Hispec Assay Diluent | ||||||
Human anti Canakinumab | HCA417 | E | 0.1 mg |
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Description | Human anti Canakinumab | ||||||
Human anti Canakinumab | HCA418 | E | 0.1 mg |
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Description | Human anti Canakinumab | ||||||
Human anti Canakinumab | HCA419 | E | 0.1 mg |
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Description | Human anti Canakinumab | ||||||
Human anti Canakinumab | TZA064 | E | 0.1 mg |
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Description | Human anti Canakinumab |
- Synonyms
- Ilaris
TZA063

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