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Canakinumab antibody | AbD54116

Human anti Canakinumab

Product Type
Monoclonal Antibody
Clone
AbD54116
Isotype
Fab antibody
Specificity
Canakinumab

Product Code Applications Pack Size List Price Your Price Qty
New TZA063
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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loader

Human Anti-Canakinumab Antibody, clone AbD54116ad is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug canakinumab, and its biosimilar products. It does not recognize the drug target, the human interleukin-1 beta (IL-1β), nor canakinumab in complex with IL-1β. This antibody can be used to measure free canakinumab levels in serum from patients.

A pair of anti-canakinumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD54116ad is recommended as a capture antibody, paired with Human Anti-Canakinumab Antibody, clone AbD54331 (TZA064) as the detection antibody.

TZA063 is a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog.

Canakinumab (trade name Ilaris) is a fully humanized monoclonal IgG1/kappa antibody with allotype G1m3. It binds to interleukin-1 beta (IL-1β) to neutralize up-regulated 1β signaling, resulting in suppression of inflammation in patients with disorders of autoimmune origin. Canakinumab is indicated for treatment of Cryopyrin-Associated Periodic Syndromes (CAPS), three rare and serious auto-inflammatory diseases, Still’s disease, and gouty arthritis.

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Canakinumab
Affinity
The monovalent intrinsic affinity of AbD54116ad was measured as KD = 9.6 nM by real time, label free molecular interaction analysis on immobilized canakinumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Ilaris is a trademark of Novartis AG.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD54116ad can be used in indirect ELISA to detect canakinumab. It can also be used as a capture antibody to develop a pharmacokinetic (PK) bridging ELISA for canakinumab, together with TZA064 or TZA064P as the detection reagent.

Protocol: PK bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Canakinumab HCA417 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Canakinumab
Human anti Canakinumab HCA418 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Canakinumab
Human anti Canakinumab HCA419 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Canakinumab
Human anti Canakinumab TZA064 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Canakinumab

Synonyms
Ilaris

TZA063

169858

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