Belimumab antibody | AbD60136ia
Human anti Belimumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD60136ia
- Isotype
- IgG1
- Specificity
- Belimumab
Antibody Quality and Performance Guarantee
A microtiter plate was coated overnight with various antigens at a concentration of 5μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human anti belimumab antibody, clone AbD60136ia (HCA430) at a concentration of 2μg/ml in HISPEC assay diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with belimumab, human IgG1 kappa control, human IgG1 lambda control at a concentration of 5%mu;g/ml or 10% human normal serum (NHS). After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human anti belimumab antibody, clone AbD60136ia (HCA430) titrated to the given concentrations in HISPEC assay diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate.
Data for belimumab is shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with belimumab at a concentration of 1μg/mL. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human anti belimumab antibody, clone AbD60138ia (HCA429, blue), clone AbD60136ia (HCA430, red), and clone AbD60132ia (HCA431, black). Detection was performed using biotin conjugated belimumab followed by HRP conjugated neutravidin and QuantaBlu fluorogenic peroxidase substrate.
Data is shown as the mean of three measurements.
A microtiter plate was coated overnight with BAFF at a concentration of 1μg/mL. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of biotin conjugated belimumab (0.15μg/mL) plus increasing concentrations of Human anti belimumab antibody, clone AbD60136ia (HCA430) in the monovalent Fab format was added. Free biotin conjugated belimumab, still capable of binding to the BAFF coated plate, was detected using HRP conjugated neutravidin followed by QuantaBlu fuorogenic peroxidase substrate. The non-inhibitory Human anti belimumab antibody, clone AbD59897ad (TZA0102) was used as a negative control.
Data is shown as the mean of three measurements.
Filter by Application:
E Reset| Human Anti-Belimumab Antibody, clone AbD60136ia is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug belimumab, and its biosimilar products. It does not recognize the drug target, the B-cell activating factor BAFF (CD257), nor belimumab in complex with BAFF. HCA430 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or calibrator in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 3nM for belimumab and is one of the three antibodies offered for ADA assays. Antibody HCA429 has an affinity of 16nM and HCA431 has an affinity of 0.2nM for belimumab. Belimumab (trade name Benlysta) is a fully human monoclonal antibody of the IgG1/lambda isotype that binds to the B-cell activating factor BAFF to prevent its binding to B cells. Although part of the normal immune system, B cells are also responsible for the over-aggressive immune response observed in autoimmune diseases, such as systemic lupus erythematosus (SLE). Through its binding to BAFF, belimumab makes BAFF unavailable to B cells, which induces B cells apoptosis and therefore, a reduction in the overall number of circulating B cells. Therefore, belimumab acts as an immunosuppressant drug and is approved for the treatment of systemic lupus erythematosus. View a summary of all anti-belimumab antibodies |
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Belimumab
- Affinity
- The monovalent intrinsic affinity of Human Anti-Belimumab Antibody, clone AbD60136ia was measured as KD = 3 nM by real time, label-free molecular interaction analysis on immobilized belimumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Benlysta is a registered trademark of GlaxoSmithKline. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient sera together with HCA429 and HCA431.
Protocol: ADA bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Belimumab | HCA429 | E | 0.1 mg |
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| Description | Human anti Belimumab | ||||||
| Human anti Belimumab | HCA431 | E | 0.1 mg |
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| Description | Human anti Belimumab | ||||||
| Human anti Belimumab | TZA0100 | E | 0.1 mg |
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| Description | Human anti Belimumab | ||||||
| Human anti Belimumab:HRP | TZA0100P | E | 0.1 mg |
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| Description | Human anti Belimumab:HRP | ||||||
| Human anti Belimumab | TZA0101 | E | 0.1 mg |
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| Description | Human anti Belimumab | ||||||
| Human anti Belimumab | TZA0102 | E | 0.1 mg |
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| Description | Human anti Belimumab | ||||||
| Human anti Belimumab:HRP | TZA0102P | E | 0.1 mg |
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| Description | Human anti Belimumab:HRP | ||||||
- Synonyms
- Benlysta
HCA430
170568
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Belimumab specificity
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