APAAP Complex antibody | WR31

APAAP Complex (Mouse)

Product Type
Monoclonal Antibody
Clone
WR31
Isotype
IgG1
Specificity
APAAP Complex

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APAAP complexes are designed for use as detection reagents in immunohistochemistry procedures. They are used in combination with a suitable bridging antibody and appropriate visualization system.

Target Species
Chemical
Product Form
Complexes of monoclonal anti-alkaline phosphatase antibody with alkaline phosphatase
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
RPMI1640 medium
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Bovine alkaline phosphatase.
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/30 1/50
Immunohistology - Paraffin 1/30 1/50
Immunohistology - Resin 1/30 1/50
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for APAAP Complex antibody

  1. Cordell, J.L. et al. (1984) Immunoenzymatic labelling of monoclonal antibodies using immune complexes of akaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J. Histochem.
    Cytochem 32: 219-229.
  2. Grau, V. et al. (1998) Dynamics of monocytes/macrophages and T lymphocytes in acutely rejecting rat renal allografts.
    Cell Tissue Res. 291: 117-26.
  3. Quenby, S. et al. (2004) Effects of low-molecular-weight and unfractionated heparin on trophoblast function.
    Obstet Gynecol. 104: 354-61.
  4. Whitworth, M.K. et al. (2007) Cervical leukocyte sub-populations in idiopathic preterm labour.
    J Reprod Immunol. 75: 48-55.

RRID
AB_322570
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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