Monensin Solution

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Figure A. RPE conjugated mouse anti human CD3 (MCA2184PE) and FITC conjugated mouse anti human IFN-gamma (MCA1581F) stained unstimulated human lymphocytes. Figure B. RPE conjugated mouse anti human CD3 (MCA2184PE) and FITC conjugated mouse anti human IFN-gamma (MCA1581F) stained human lymphocytes stimulated with PMA and ionomycin for 5 hours in the presence of monensin solution (BUF074). Data acquired on the ZE5™ Cell Analyzer.

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  • Monensin Solution (1000X)
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF074Fdatasheet pdfdatasheet pdf1 ml
    BUF074
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Monensin Solution (1000x) is commonly used to enhance intracellular cytokine staining by inhibiting protein transport. This solution causes many of the cytokines to accumulate in the Golgi apparatus and endoplasmic reticulum.
    • Intended Use
    • Product Form
      1000x concentrate - liquid
    • Reconstitution
    • Preparation
      2 mM solution prior to dilution
    • Preservative Stabilisers
      70% Ethanol
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.

      Ethanol is flammable, keep away from sources of fire.
    • Shelf Life
      Please see label for expiry date
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      1. Dilute the Monensin Solution (1000x) to a 1x working concentration using cell culture media
      2. Add Monensin Solution (1x) to cell culture for 4-24 hours prior to antibody staining. Do not exceed cell culture time as this may affect cell viabiilty

      Note: Time and cell culture conditions may need to be optimized

    Additional Monensin Solution Formats

    Formats Applications Sizes available
    Monensin Solution : Reagent F 1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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