CytoTrack Cell Proliferation Assays

CytoTrack™ Cell Proliferation Assays

Resolve up to ten cell divisions

CytoTrack cell proliferation assays efficiently stain live cells to obtain excellent resolution of each cell division generation. CytoTrack dyes allow you to measure cell proliferation in live cells. As the labeled cells divide, the concentration of the dye is halved and the proliferation can be measured based upon the reduced levels of fluorescence in subsequent generations. These dyes are available in a range of excitation and emission spectra for convenient incorporation into multi-color Flow Cytometry panels.

For convenient CFSE staining, we also offer a cell proliferation assay featuring CFDA-SE dye to monitor proliferation.

Excellent resolution of ten cell generations using CytoTrack green 511/525 (A) compared to CFDA-SE (B).

Figure 1. Excellent resolution of ten cell generations using CytoTrack green 511/525 (A) compared to CFDA-SE (B). 


CytoTrack cell proliferation dyes key benefits

The benefits of using CytoTrack cell proliferation dyes are numerous and these include:

  • Detect intracellular targets - Cytotrack is compatible with standard formaldehyde-containing fixatives and saponin-based permeabilization buffers
  • Suitable for multicolor cell analysis: Range of excitations and emissions for compatibility with fluorescent proteins or fluorophore-labeled antibodies
  • No washing required, use the cell proliferation assay directly with your cell culture media
  • Resolve up to 10 cell divisions compared to CFSE

Proliferation Dyes

Description

Excitation Max, nm

Emission Max, nm

Optimal Excitation Laser

Catalog Number

CytoTrack blue

403

454

405

1351202

CytoTrack green

511

525

488

1351203

CytoTrack yellow

542

556

532

1351204

CytoTrack red

628

643

640

1351205

CFDA-SE

494

521

488

1351201


We also have reagents and kits to detect and measure proliferation, suitable for use in ELISA, Microscopy and Flow Cytometry as well as antibodies to detect common cell proliferation markers and BrdU incorporation into newly synthesized DNA.