Caspase-9

SR FLICA™ Caspase-9 Kit

Product Type
Kits
Specificity
Caspase-9

Product Code Applications Pack Size List Price Your Price Qty
ICT961
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 100 Tests
List Price Your Price
Search for Batch Specific Datasheets

The SR FLICA Caspase 9 kit uses a quick and easy method to analyze active caspases in apoptotic cells.

Reagents in the Kit
4 vials of SR-LEHD-FMK FLICA Reagent - lyophilized
10X Wash Buffer, 60 mL
Fixative, 6 mL
Hoechst Stain, 1 ml.
Test Principle
Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a red (Sulforhodamine, SR) fluorescent probe.

The Caspase FLICA Kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

This kit can be used with a fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Sulforhodamine 565 590
Regulatory
For research purposes only.
Guarantee
Guaranteed until date of expiry. Please see product label.
Acknowledgements
FLICA is a trademark of Immunochemistry Technologies, LLC.

Store the unopened kit (and each unopened component) at +4°C until the expiration date.
Protect the FLICA reagent from light at all times.

Once reconstituted, the 150X FLICA stock should be stored at -20°C protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT961.pdf

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
SR FLICA™ Caspase-9 Kit ICT960 F IF 25 Tests
List Price Your Price
Description SR FLICA™ Caspase-9 Kit
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"