Caspase-3/7

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FLICA™ 660 Caspase-3/7 Kit

Product Type
Kits
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
ICT9125 F IF 25 - 50 Tests
The FLICA 660 Caspase-3/7 kit uses a target sequence (DEVD) sandwiched between a far-red fluorescent 660 dye and a fluoromethylketone (FMK) to make a quick and flexible method to analyze active caspase-3/7 in apoptotic cells.

Product Details

Reagents in the Kit
1 vial of 660-DEVD-FMK FLICA Reagent - lyophilized
10x Wash Buffer, 15 mL
Fixative, 6 mL
Test Principle
Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a far-red fluorescent 660 dye.

The Caspase FLICA Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. This kit can be used with either a flow cytometer or fluorescence microscope.

Storage Information

Storage
Store the unopened kit (and each unopened component) at +4oC until the expiration date.
Protect the FLICA reagent from light at all times.

Once reconstituted in DMSO, the FLICA stock should be stored at -20°C protected from light.
Shelf Life
Please see label for expiry date

More Information

Acknowledgements
FLICA is a trademark of Immunochemistry Technologies, LLC.
Regulatory
For research purposes only

Applications of Caspase-3/7

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT9125.pdf

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
FLICA™ 660 Poly Caspase Kit ICT9120 25 - 50 Tests F IF
FLICA™ 660 Caspase-1 Kit ICT9122 25 - 50 Tests F IF