Browsing Modes for Business Users

Annexin V Kit

ISO 9001 Quality Assurance Manufacturing | Print
Annexin V Kit thumbnail image 1 Annexin V Kit thumbnail image 2 Annexin V Kit thumbnail image 3 Annexin V Kit thumbnail image 4 Annexin V Kit thumbnail image 5 Annexin V Kit thumbnail image 6 Annexin V Kit thumbnail image 7 Annexin V Kit thumbnail image 8
Annexin V Kit gallery image 1
Jurkat T cells were treated as shown with staurosporine at 1 µM to induce apoptosis. The cells were then stained with Annexin V:SBB675 Kit (ANNEXINSBB675) and ReadiDrop Propidium Iodide (1351101). Apoptotic cells positive for annexin V can be seen in the bottom right quadrant and dead cells positive for both annexin and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyzer.
Annexin V Kit gallery image 2
Jurkat T cells were treated as shown with staurosporine at 1 µM to induce apoptosis. The cells were then stained with Annexin V:SBB765 Kit (ANNEXINSBB765) and ReadiDrop Propidium Iodide (1351101). Apoptotic cells positive for annexin V can be seen in the bottom right quadrant and dead cells positive for both annexin and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyzer.
Annexin V Kit gallery image 3
Jurkat T cells were treated as shown with staurosporine at 1 µM to induce apoptosis. The cells were then stained with Annexin V:SBY800 Kit (ANNEXINSBY800) and ReadiDrop Propidium Iodide (1351101). Apoptotic cells positive for annexin V can be seen in the bottom right quadrant and dead cells positive for both annexin and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyzer.
Annexin V Kit gallery image 4
Jurkat T cells were treated as shown with staurosporine at 1 µM to induce apoptosis. The cells were then stained with Annexin V:SBV790 Kit (ANNEXINSBV790) and ReadiDrop Propidium Iodide (1351101). Apoptotic cells positive for annexin V can be seen in the bottom right quadrant and dead cells positive for both annexin and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyzer.
Annexin V Kit gallery image 5
Jurkat T cells were treated as shown with staurosporine at 1 µM to induce apoptosis. The cells were then stained with Annexin V:SBV440 Kit (ANNEXINSBV440) and ReadiDrop Propidium Iodide (1351101). Apoptotic cells positive for annexin V can be seen in the bottom right quadrant and dead cells positive for both annexin and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyzer.
Annexin V Kit gallery image 6
Jurkat T cells were treated as shown with staurosporine at 1 µM to induce apoptosis. The cells were then stained with Annexin V:SBV515 Kit (ANNEXINSBV515) and ReadiDrop Propidium Iodide (1351101). Apoptotic cells positive for annexin V can be seen in the bottom right quadrant and dead cells positive for both annexin and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyzer.
Annexin V Kit gallery image 7
Jurkat T cells were treated as shown with staurosporine at 1 µM to induce apoptosis. The cells were then stained with Annexin V:SBUV400 Kit (ANNEXINSBUV400) and ReadiDrop Propidium Iodide (1351101). Apoptotic cells positive for annexin V can be seen in the bottom right quadrant and dead cells positive for both annexin and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyzer.
Annexin V Kit gallery image 8
Jurkat T cells were treated as shown with staurosporine at 1 µM to induce apoptosis. The cells were then stained with Annexin V:SBUV795 Kit (ANNEXINSBUV795) and ReadiDrop Propidium Iodide (1351101). Apoptotic cells positive for annexin V can be seen in the bottom right quadrant and dead cells positive for both annexin and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyzer.

Annexin V:StarBright Blue 675 Kit

Product Type
Kits
Specificity
Annexin V Kit

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
New ANNEXINSBB675
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Annexin V Kit allows fluorescent detection of annexin V bound to cells undergoing apoptosis. Apoptosis, or programmed cell death, is an essential process, by which organisms ensure that critically damaged or mutated cells are removed. This process is highly regulated by a complex interplay of proteins.

During the early stages of apoptosis, the plasma membrane of a cell is rearranged, resulting in changes to the cell membrane’s phospholipid asymmetry. Phosphatidylserine (PS), a phospholipid located within the cell membrane, is translocated and displayed on the outer plasma membrane (Fadeel and Xue, 2009). Annexin V; a protein encoded by the ANXA5 gene, has a high binding affinity for PS in the presence of Ca2+, and is therefore used as a tool for detecting early apoptotic cells.

The conjugation protocol used to prepare this product has not changed the native phospholipid binding properties of annexin V. This protocol is designed to measure apoptosis easily and quickly in a sample of suspended cells.

View our complete list of Annexin V Kits

Preservative Stabilisers
Annexin V:SBB675
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20

10x Binding Buffer
None present
Reagents In The Kit
1 Vial of Annexin V:SBB675, 100 tests/0.5 ml
1 Bottle of 10x Binding Buffer, 20 ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Blue 675 476 675
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
ANNEXINSBB675
1) Dilute the 10x Binding Buffer to 1x in distilled water (1 ml 10x Binding Buffer + 9 ml distilled water).

2) Centrifuge and resuspend cells in PBS, adjusting to a cell density of 1 x 106 cells/ml.

3) Transfer 100 μl cells to a 96-well flat bottom plate.

4) Centrifuge and resuspend cells in 95 μl 1x Binding Buffer.

5) Add 5 μl of SBB675 conjugated Annexin V

6) Mix and incubate for 10 minutes at room temperature in the dark.

7) Centrifuge and resuspend cells in 200 μl of 1x Binding Buffer.

8) Resuspend cells in 200 μl of 1x Binding Buffer and add viability dye of choice at previously optimized concentration (e.g. DAPI or PI at 1 μg/ml).

9) Analyze by flow cytometry immediately. If not analyzed immediately, store at 2-8°C in the dark for maximum 4 hours, experimental design permitting.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Annexin V:StarBright Blue 765 Kit ANNEXINSBB765 F 100 Tests loader
List Price Your Price
loader
Description Annexin V:StarBright Blue 765 Kit
Annexin V:StarBright UltraViolet 400 Kit ANNEXINSBUV400 F 100 Tests loader
List Price Your Price
loader
Description Annexin V:StarBright UltraViolet 400 Kit
Annexin V:StarBright UltraViolet 795 Kit ANNEXINSBUV795 F 100 Tests loader
List Price Your Price
loader
Description Annexin V:StarBright UltraViolet 795 Kit
Annexin V:StarBright Violet 440 Kit ANNEXINSBV440 F 100 Tests loader
List Price Your Price
loader
Description Annexin V:StarBright Violet 440 Kit
Annexin V:StarBright Violet 515 Kit ANNEXINSBV515 F 100 Tests loader
List Price Your Price
loader
Description Annexin V:StarBright Violet 515 Kit
Annexin V:StarBright Violet 790 Kit ANNEXINSBV790 F 100 Tests loader
List Price Your Price
loader
Description Annexin V:StarBright Violet 790 Kit
Annexin V:StarBright Yellow 800 Kit ANNEXINSBY800 F 100 Tests loader
List Price Your Price
loader
Description Annexin V:StarBright Yellow 800 Kit

UniProt
P08758
Entrez Gene
ANXA5
GO Terms
GO:0005509 calcium ion binding
GO:0007596 blood coagulation
GO:0004859 phospholipase inhibitor activity
GO:0007165 signal transduction
GO:0005544 calcium-dependent phospholipid binding
GO:0005737 cytoplasm
GO:0006916 anti-apoptosis
GO:0050819 negative regulation of coagulation

ANNEXINSBB675

64623687

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with ANNEXIN V KIT specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

Please note