Annexin V Kit

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Annexin V:APC Assay Kit

Annexin V:Pe Assay Kit

Product Type
Kits
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
ANNEX200APC F 200 Tests
ANNEX200PE F 200 Tests
This test employs the property of Annexin V to bind to the membrane phospholipid phosphatidylserine (PS) in the presence of Ca2+. PS is exposed at the cell surface during the early stages of apoptosis. Detection of PS is a very sensitive method for detecting cells entering apoptosis, at a time point considerably ahead of nuclear changes such as DNA degradation.

The conjugation protocol used to prepare this product has not changed the native phospholipid binding properties of Annexin V. This protocol is designed to measure apoptosis easily and quickly in a sample of suspended cells.

View our complete list of formats and sizes of Annexin V kits

Product Details

Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Reagents in the Kit
Annexin V:APC200 tests
Propidium Iodide Staining Solution2X 100 tests
10X Binding Buffer100 ml
Reagents in the Kit
Annexin V:PE200 tests
7-AAD Viability Staining Solution2X 100 tests
10X Binding Buffer100 ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P08758 Related reagents
Entrez Gene
ANXA5 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0007596 blood coagulation
GO:0004859 phospholipase inhibitor activity
GO:0007165 signal transduction
GO:0005544 calcium-dependent phospholipid binding
GO:0005737 cytoplasm
GO:0006916 anti-apoptosis
GO:0050819 negative regulation of coagulation
Regulatory
For research purposes only

Applications of Annexin V Kit

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
1) Dilute the10X binding buffer to 1X in distilled water (1 ml binding buffer + 9 ml distilled water).

2) Wash cells once in PBS by gentle shaking or pipetting up and down. Then wash once in 1X binding buffer.

3) Resuspend cells in 1X binding buffer, adjusting to a cell density of 1-5 x 106 cells/ml.

4) Add 5 μl of APC conjugated Annexin V to 100 μl of the cell suspension.

5) Mix and incubate for 10 to15 minutes at room temperature.

6) Wash cells in 1X binding buffer.

7) Resuspend cells in 200 μl of 1X binding buffer.

8) Add 5 μl of Propidium Iodide Staining Solution.

9) Analyse by flow cytometry within 4 hours. Store at 2-8°C in the dark.
Instructions For Use
1) Dilute the10X Binding Buffer to 1X in distilled water (1 ml Binding Buffer + 9 ml distilled water).

2) Wash cells once in PBS by gentle shaking or pipetting up and down. Then wash once in 1X Binding Buffer.

3) Resuspend cells in 1X Binding Buffer, adjusting to a cell density of 1-5 x 106 cells/ml.

4) Add 5 μl of PE conjugated Annexin V to 100 μl of the cell suspension.

5) Mix and incubate for 10 to15 minutes at room temperature.

6) Wash cells in 1X Binding Buffer.

7) Resuspend cells in 200 μl of 1X Binding Buffer.

8) Add 5 μl of 7-AAD Viability Staining Solution and incubate for 5 minutes at room temperature.

9) Analyse by flow cytometry within 4 hours. Store at 2-8°C in the dark.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Annexin V:Biotin Assay Kit ANNEX100B 100 Tests F
Annexin V:FITC Assay Kit ANNEX100F 100 Tests F
Annexin V:Pe Assay Kit ANNEX200PE 200 Tests F
Annexin V:Biotin Assay Kit ANNEX20B 20 Tests F
Annexin V:FITC Assay Kit ANNEX20F 20 Tests F
Annexin V:FITC Assay Kit ANNEX300F 300 Tests F
Annexin V:APC Assay Kit ANNEX50APC 50 Tests F
Annexin V:Pe Assay Kit ANNEX50PE 50 Tests F
Annexin V:Biotin Assay Kit ANNEX100B 100 Tests F
Annexin V:FITC Assay Kit ANNEX100F 100 Tests F
Annexin V:APC Assay Kit ANNEX200APC 200 Tests F
Annexin V:Biotin Assay Kit ANNEX20B 20 Tests F
Annexin V:FITC Assay Kit ANNEX20F 20 Tests F
Annexin V:FITC Assay Kit ANNEX300F 300 Tests F
Annexin V:APC Assay Kit ANNEX50APC 50 Tests F
Annexin V:Pe Assay Kit ANNEX50PE 50 Tests F