Ics Perm/Wash Buffer

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  • Ics Perm/Wash Buffer (10X)
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF072Fdatasheet pdfdatasheet pdf100 ml
    BUF072
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • ICS Perm/Wash Buffer allows access to intracellular targets such as cytokines and cytoplasmic proteins which require permeabilization of the cell membrane after fixation. This permeabilization buffer has minimal effect on cell morphology and is supplied as a 10x solution for dilution in water. It can also be used as a wash buffer during intracellular staining and to dilute antibodies during intracellular staining to help minimize background staining. ICS Perm/Wash buffer can also be used as a wash buffer during intracellular staining and to dilute antibodies during intracellular staining to help reduce background staining.
    • Intended Use
    • Product Form
      10x concentrate - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
      Crystallization may occur at the reccomended storage conditions, this is normal and does not affect buffer performance. If crystallization remains after dilution to 1X soluiton, the buffer can be filtered
    • Shelf Life
      12 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      1. Dilute the ICS Perm/Wash Buffer (10x) to a 1x working concentration using DI water
      2. Resuspend fixed cells in ICS Perm/Wash buffer (1x)
      3. Centrifuge cells at 350 g for 5-10 min. Repeat twice
      4. Stain cells for intracellular targets
      It is recomended that the reagent be titrated for optimal performance for each application.

    Additional Ics Perm/Wash Buffer Formats

    Formats Applications Sizes available
    Ics Perm/Wash Buffer : Reagent F 100 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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