WNT-3 Antibody

WNT-3 Antibody gallery image 1

Western blotting analysis of HEK293 cell line lysate (35μg protein in RIPA buffer) probed with Goat anti Human WNT-3 antibody (AHP2268) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

WNT-3 Antibody gallery image 2

Immunohistochemical staining of paraffin embedded Human Breast tissue sections probed with Goat anti mouse WNT-3 antibody (AHP2268) at 2.0μgml-1 following heat mediated antigen retrieval with citrate buffer pH 6.0

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  • Goat anti WNT-3
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2268E, IF, P*, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Wnt-3 antibody recognises both human and mouse proto-oncogene Wnt-3, a member of the Wnt family, and ligand of the frizzled family of seven transmembrane receptors.

      Wnt-3, otherwise known as Int-4, is involved in cell fate decisions and patterning during embryogenesis, cell signalling during neural tube development, and implicated in the tumorogenesis of several cancers including breast, lung, hepatocellular(HCC), and gastric carcinoma.

      Over-expression of Wnt-3 playes a pivotal role in the resistance of HER2-overexpressing breast tumors to the breast cancer treatment drug Trastuzumab, also known as Herceptin (Wu et al. 2012). The effects of Wnt-3 over-expression included the transactivated expression of EGFR (epidermal growth factor receptor) an increased expression of B-catenin within the nucleus, an increase in N-cadherin and the neural crest transcription factor SLUG, as well as promoting an EMT (epithelial to mesenchymal transition) response.

      Goat anti Wnt-3 polyclonal antibody exhibits strong fibrous staining in myoepithelial cells of human breast tissue in paraffin sections.

      Goat anti Wnt-3 polyclonal antibody has been shown to stain mouse hippocampal primary astrocytes in ICC/IF studies, which investigated the role of Wnt-3 levels and Wnt-3 expressing astrocytes, in the regulation of age-associated decline in adult neurogenesis (Okamoto et al. 2011).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1.03.0ug/ml
      Immunohistology - Paraffin(1)3.05.0ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human breast
    • Immunofluorescence
    • Western Blotting
      AHP2268 detects a band of approximately 40kDa in HEK293 cell lysates
    • Instructions For Use

    Additional WNT-3 Antibody Formats

    Formats Applications Sizes available
    WNT-3 Antibody : Purified E, IF, P*, WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Human breast

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