Goat anti Human Troponin I antibody recognizes human cardiac-specific troponin I, (TnI) an integral inhibitory protein of cardiac muscle which exists as a complex with troponin C (TnC) and troponin T (TnT).
The TnT subunit of troponin binds to tropomyosin to form a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, enabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement.
The measurement of blood TnI and TnT levels is an important diagnostic indicator of heart muscle damage, and can be used to differentiate between angina and myocardic infarction in patients with chest pain. Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro.
Purified IgG - liquid
Purified IgG prepared by Immunoaffinity chromatography
Antisera to human cardiac troponin I were raised by repeated immunisations of goats with highly purified antigen.
Sodium Azide (NaN3)
Native human cardiac troponin I, purity greater than 98%.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
1. Jin, J. et al. (2009) Transplantation of mesenchymal stem cells within a poly(lactide-co-epsilon-caprolactone) scaffold improves cardiac function in a rat myocardial infarction model.Eur J Heart Fail. 11: 147-53.
2. Demyanets, S. et al. (2013) Components of the interleukin-33/ST2 system are differentially expressed and regulated in human cardiac cells and in cells of the cardiac vasculature.J Mol Cell Cardiol. 60: 16-26.