TR3 Orphan Receptor Antibody

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Human thyroid cell lysate probed with Goat anti Human TR3 orphan receptor (AHP2139)

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  • Goat anti Human TR3 Orphan Receptor
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2139E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2139
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • AHP2139 specifically recognises human TR3 orphan receptor, otherwise known as NUR77, an immediate-early orphan nuclear receptor, and member of the nuclear receptor hormone family, encoded by the NR4A1 gene.

      TR3 orphan receptor plays a role in the mediation of macrophage inflammatory responses, and in apoptosis and cell proliferation, through the binding to a variety of response elements. TR3 orphan receptor has been shown to bind to the anti-apoptotic protein Bcl-2, and can be induced by both physiological and physical stress-related stimuli, including tumour necrosis factor (TNF), inflammatory cytokines and magnetic fields.

      AHP2139 is expected to recognise isoform a of TR3 orphan receptor.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      DogExpected from Sequence
      MouseExpected from Sequence
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human TR3 orphan receptor was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence C-GRLPSKPKQPPDAS from the internal region of TR3 orphan receptor (NP_002126.2; NP_775180.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      nucleoplasm
      regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
      zinc ion binding
      nerve growth factor receptor signaling pathway
      phosphatidylinositol-mediated signaling
      steroid hormone receptor activity
      sequence-specific DNA binding transcription factor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/32000
      Western Blotting1.03.0ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP2139 detects a band of approximately 60kDa in human thyroid gland cell lysates.
    • Instructions For Use

    Additional TR3 Orphan Receptor Antibody Formats

    Formats Applications Sizes available
    TR3 Orphan Receptor Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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