Sandwich ELISA analysis of human thyroglobulin using Mouse anti Human thyroglobulin , clone 5F9 (8900-0700) as a capture reagent and biotinylated Mouse anti Human thyroglobulin, clone 5E6 (8900-0730) as a detection reagent with purified human thyroglobulin as antigen (PHP236) for production of a standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples (green and blue) and plasma samples (orange and red) are shown
Purified protein from human thyroid glands - liquid
SDS PAGE: >98%, ultra-pure, IgG low
Approx. Protein Concentrations
660 kDa, confirmed by chromatography and electrophoresis. Under certain conditions the subunit molecular weight may be observed (330 kDa).
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Please view the datasheet pdf for suggested dilutions.