Thyroglobulin

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Sandwich ELISA analysis of human thyroglobulin using Mouse anti Human thyroglobulin , clone 5F9 (8900-0700) as a capture reagent and biotinylated Mouse anti Human thyroglobulin, clone 5E6 (8900-0730) as a detection reagent with purified human thyroglobulin as antigen (PHP236) for production of a standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples (green and blue) and plasma samples (orange and red) are shown

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  • Native Human Thyroglobulin
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  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP236Edatasheet pdfdatasheet pdf1 mg
    PHP236
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified protein from human thyroid glands - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      None present.
    • Purity
      SDS PAGE: >98%, ultra-pure, IgG low
    • Approx. Protein Concentrations
      1.0 mg/ml
    • Molecular Weight
      660 kDa, confirmed by chromatography and electrophoresis. Under certain conditions the subunit molecular weight may be observed (330 kDa).
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Thyroglobulin Formats

    Formats Applications Sizes available
    Thyroglobulin : Purified E 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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