PP1 Alpha Antibody (PrecisionAbTM Antibody) | OTI6E5

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PP1 Alpha Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI6E5 gallery image 1

Western blot analysis of whole cell lysates probed with PP1 alpha antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 9 second exposure. Arrow points to PP1 alpha (molecular weight 38 kDa)

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  • Mouse anti PP1 Alpha
  • PP1 Alpha Antibody with Control Lysate
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    PP1 alpha antibody recognizes PP1 alpha, also known as serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00742WBdatasheet pdfdatasheet pdf100 µl
      VMA00742
      VMA00742KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00742KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human PP1 alpha antibody recognizes PP1 alpha, also known as protein phosphatase 1-alpha isoform and serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit.

        The protein encoded by PPP1CA is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for PPP1CA (provided by RefSeq, Jul 2008).

        Mouse anti Human PP1 alpha antibody detects a band of 38 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Mouse anti PP1 alpha detects a band of approximately 38 kDa in Jurkat cell lysates
        Mouse anti PP1 alpha detects a band of approximately 38 kDa in Jurkat cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - Liquid
        Purified IgG - Liquid
      • Preparation
        Mouse monoclonal antibody affinity purified on Protein G from ascites
        20μl Mouse monoclonal antibody affinity purified on Protein G from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide
        1% Bovine Serum Albumin
        0.09% Sodium Azide
        1% Bovine Serum Albumin
      • Immunogen
        E. coli-derived full length recombinant protein of human PP1 alpha
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20°C, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        cell cycle
        MLL5-L complex
        cytosol
        glycogen metabolic process
        protein dephosphorylation
        protein serine/threonine phosphatase activity
        PTW/PP1 phosphatase complex
        metal ion binding
        triglyceride catabolic process
        protein phosphatase type 1 regulator activity
        protein binding
        nucleolus
        cell division
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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