Microtubule Associated Protein tau Antibody (PrecisionAbTM Antibody)

Microtubule Associated Protein tau Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with microtubule associated protein TAU antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to microtubule associated protein tau (molecular weight 50-80 kDa)

  • Microtubule Associated Protein tau Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Rabbit anti Microtubule Associated Protein tau
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  • Product Type
    PrecisionAb™ Polyclonal
  • Isotype
    Polyclonal IgG
  • Format
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VPA00677WBdatasheet pdfdatasheet pdf100 µl
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    • Rabbit anti Human microtubule associated protein tau antibody recognizes the microtubule associated protein tau, also known as G protein beta1/gamma2 subunit-interacting factor 1, PHF-tau, neurofibrillary tangle protein, paired helical filament-tau or protein phosphatase 1, regulatory subunit 103.

      The MAPT gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy (provided by RefSeq, Jul 2008).

      Rabbit anti Human microtubule associated protein tau antibody detects a band of 50-80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
    • Western Blotting
      Rabbit anti microtubule associated protein tau detects a band of approximately 50-80 kDa in SH-SY5Y cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Preparation
      Rabbit polyclonal antibody purified by affinity chromatography
    • Buffer Solution
      Phosphate buffered saline
    • Preservative Stabilisers
      0.01% Thimerosal
      1% Bovine Serum Albumin
      <50% Glycerol
    • Immunogen
      Recombinant protein encompassing part of the middle region of human microtubule associated protein tau
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
    • Lysate Reconstitution
    • Storage
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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