Human anti Human Megalin antibody, clone 11723 recognizes human Megalin, also known as LRP-2 and Glycoprotein 330, a transmembrane protein with an approximate molecular weight of 521 kDa belonging to the LDLR family.
Megalin is a multi-ligand binding receptor involved in the mediation of various cell processes including the binding of plasminogen, lactoferin and various extracellular matrix components. In association with RAP, Megalin forms the Heymann nephritis antigenic complex. Magalin is located in absorptive epithelia including the renal proximal tubules.
A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with V5 and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
Reconstitute with 1.0ml distilled water
StrepTactin affinity chromatography
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin
Human Megalin (aa sequence 1024-1224) – N1 fusion protein.
Approx. Protein Concentrations
Total protein concentration 0.1mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.