Megalin Antibody | 11723

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Citrate treated formalin fixed paraffin embedded human kidney stained with Human anti Human Megalin (HCA146) followed by mouse anti Strep-tag (MCA2488) and histar detection (STAR3000A)

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  • Megalin Antibody | 11723 thumbnail image 1
  • Human anti Human Megalin
  • Human anti Human Megalin
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  • Product Type
    Monoclonal Antibody
  • Clone
    11723
  • Isotype
    HuCAL Fab monovalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA146Pdatasheet pdfdatasheet pdf0.1 mg
    HCA146
    HCA146APdatasheet pdfdatasheet pdf50 µg
    HCA146A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Human Megalin antibody, clone 11723 recognizes human Megalin, also known as LRP-2 and Glycoprotein 330, a transmembrane protein with an approximate molecular weight of 521 kDa belonging to the LDLR family.

      Megalin is a multi-ligand binding receptor involved in the mediation of various cell processes including the binding of plasminogen, lactoferin and various extracellular matrix components. In association with RAP, Megalin forms the Heymann nephritis antigenic complex. Magalin is located in absorptive epithelia including the renal proximal tubules.
    • Intended Use
    • Target Species
      Human
    • Product Form
      A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with V5 and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with V5 and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Human Megalin (aa sequence 1024-1224) – N1 fusion protein.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Pack Size: 0.1 mgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      SH3 domain binding
      coated pit
      calcium ion binding
      receptor activity
      vitamin D metabolic process
      protein glycosylation
      hormone biosynthetic process
      receptor-mediated endocytosis
      integral to membrane
      lysosome
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1:501:100
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1:501:100

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Histology Positive Control Tissue
      Human kidney
    • Histology Positive Control Tissue
      Human kidney
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Megalin Antibody Formats

    Formats Clone Applications Sizes available
    Megalin Antibody : Purified 11723 P 50 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti V5-Tag:Alk. Phos.MCA1360A0.1 mgE, WB
      MCA1360A
      Mouse anti Strep-Tag ImmoMCA24880.1 mgE
      MCA2488
      Mouse anti V5-TagMCA13601 mgC, E, F, IF, IP, R, WB
      MCA1360
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti V5-Tag:Alk. Phos.MCA1360A0.1 mgE, WB
      MCA1360A
      Mouse anti Strep-Tag ImmoMCA24880.1 mgE
      MCA2488
      Mouse anti V5-TagMCA13601 mgC, E, F, IF, IP, R, WB
      MCA1360

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B

          Recommended Positive Controls

            Histology Controls

              Human kidney
              Human kidney

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