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Mouse anti Human Laminin Alpha 5 antibody, clone 4C7 (5620-0436) used for the identification of alpha-5 laminin from JAR choriocarcinoma cells by immunopercipitation.
Expression and synthesis of laminins by JAR cells.
A) JAR choriocarcinoma cells transcribe lm α1, lm α3, lm α5, lm β1, lm β2, lm β, lm γ2, and lm γ1 chains as shown by RT-PCR. As a reference sample, we used optimized, in-house prepared positive control mix, which contains cDNA from undifferentiated hPSCs, spontaneously differentiated hPSCs as well as from isolated human pancreatic islets. B) JAR choriocarcinoma cells were metabolically labeled with 35S and the cell culture supernatant and matrix deposited by JAR cells were immunoprecipitated using antibodies, which specifically recognize the human lm α1 (400 kDa) and lm α5 (350-380 kDa) chains. The cells showed abundant lm-511 and -111 synthesis.
From: Vuoristo S, Toivonen S, Weltner J, Mikkola M, Ustinov J, Trokovic R, et al. (2013)
A Novel Feeder-Free Culture System for Human Pluripotent Stem Cell Culture and Induced Pluripotent Stem Cell Derivation.
PLoS ONE 8(10): e76205.