Laminin Antibody | 4C7 (2D8/33)

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Laminin Antibody | 4C7 (2D8/33) gallery image 1

Published investigator image:
Mouse anti Human Laminin Alpha 5 antibody, clone 4C7 (5620-0436) used for the identification of alpha-5 laminin from JAR choriocarcinoma cells by immunopercipitation.
Image caption:
Expression and synthesis of laminins by JAR cells.
A) JAR choriocarcinoma cells transcribe lm α1, lm α3, lm α5, lm β1, lm β2, lm β, lm γ2, and lm γ1 chains as shown by RT-PCR. As a reference sample, we used optimized, in-house prepared positive control mix, which contains cDNA from undifferentiated hPSCs, spontaneously differentiated hPSCs as well as from isolated human pancreatic islets. B) JAR choriocarcinoma cells were metabolically labeled with 35S and the cell culture supernatant and matrix deposited by JAR cells were immunoprecipitated using antibodies, which specifically recognize the human lm α1 (400 kDa) and lm α5 (350-380 kDa) chains. The cells showed abundant lm-511 and -111 synthesis.

From: Vuoristo S, Toivonen S, Weltner J, Mikkola M, Ustinov J, Trokovic R, et al. (2013)
A Novel Feeder-Free Culture System for Human Pluripotent Stem Cell Culture and Induced Pluripotent Stem Cell Derivation.
PLoS ONE 8(10): e76205.

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Laminin Antibody | 4C7 (2D8/33) gallery image 2

Published investigator image:
Mouse anti Human Laminin Alpha 5 antibody, clone 4C7 (5620-0436) used for the identification of lamanin alpha-5 by western blotting.
Image caption:
Reactivity of novel mAbs to LMα5 chain as measured by ELISA, Western blotting and immunoprecipitation. A) Reactivity of the antibodies with rhLMs 411, 511 and 521 by ELISA. B) Reactivity of the antibodies with rhLM511 by Western blotting under reducing conditions. C) Ability of the antibodies to immunoprecipitate laminin-511 from A549 cells' conditioned medium and detection of the LMα5 chain by Western blotting with mAb 4B5. Bands of 300/350 kDa corresponding to LMα5 chain were detected with some of the antibodies.

From: Citation: Wondimu Z, Omrani S, Ishikawa T, Javed F, Oikawa Y, Virtanen I, et al. (2013)
A Novel Monoclonal Antibody to Human Laminin α5 Chain Strongly Inhibits Integrin-Mediated Cell Adhesion and Migration on Laminins 511 and 521.
PLoS ONE 8(1): e53648.

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Laminin Antibody | 4C7 (2D8/33) gallery image 3

Published investigator image:
Mouse anti Human Laminin Alpha 5 antibody, clone 4C7 (5620-0436) used for the evaluation of lamanin alpha-5 expression in human kidney by immunohistofluorescence on acetone fixed cryosections.
Image caption:
Immunohistochemical reactivity of mAb 8G9 in comparison to mAb 4C7 against LMa5 chain.
(A) and (C), mAb 4C7; (B) and (D), mAb 8G9 against frozen sections of human kidney (A) and (B), and skin (C) and (D). The staining pattern of the two antibodies was indistinguishable. In kidney, the antibodies stained glomerular and tubular basement membranes, whereas in skin, reactivity with epidermal and vascular basement membranes was observed.

From: Citation: Wondimu Z, Omrani S, Ishikawa T, Javed F, Oikawa Y, Virtanen I, et al. (2013)
A Novel Monoclonal Antibody to Human Laminin α5 Chain Strongly Inhibits Integrin-Mediated Cell Adhesion and Migration on Laminins 511 and 521.
PLoS ONE 8(1): e53648.

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  • Mouse anti Human Laminin Alpha 5
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    4C7 (2D8/33)
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    5620-0436E, IF, IPdatasheet pdfdatasheet pdf50 µl
    5620-0436
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Laminin Alpha 5 antibody, clone 4C7 recognizes the laminin alpha 5 chain also known as Laminin-10 subunit alpha, Laminin-11 subunit alpha or Laminin-15 subunit alpha. Laminin is a complex glycoprotein composed of three polypeptide chain complexes. Laminin alpha 5 is expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta, there is little or no expression in brain and liver.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunofluorescence
      Immunoprecipitation
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Laminin Antibody Formats

    Formats Clone Applications Sizes available
    Laminin Antibody : Ascites 4C7 (2D8/33) E, IF, IP 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence
                Immunohistology - Frozen

              References

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