Kinesin Heavy Chain Antibody (PrecisionAbTM Antibody)

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Kinesin Heavy Chain Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with kinesin heavy chain antibody followed by detection with HRP conjugated Rabbit anti Goat IgG (Fc) (1/10,000, STAR122P) and visualized on the ChemiDoc MP with 300 second exposure. Arrow points to kinesin heavy chain (molecular weight 110 kDa)

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  • Kinesin Heavy Chain Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Kinesin Heavy Chain Antibody with Control Lysate
  • Goat anti Kinesin Heavy Chain
  • Kinesin Heavy Chain Antibody with Control Lysate
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    Kinesin heavy chain antibody recognizes kinesin-1 heavy chain, also known as conventional kinesin heavy chain, ubiquitous kinesin heavy chain or UKHC. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00053WBdatasheet pdfdatasheet pdf100 µl
      VPA00053
      VPA00053KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00053K
      VPA00053KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00053KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human kinesin heavy chain antibody recognizes the heavy chain of human kinesin, also known as kinesin-1 heavy chain, conventional kinesin heavy chain, ubiquitous kinesin heavy chain or KIF5B.

        Kinesin heavy chain is a 963 amino acid microtubule dependent motor protein. This protein is a member of the kinesin-like protein family and principal component of kinesin, involved in the plus end-directed transport of eukaryotic cell vesicles and organelles.

        Kinesins are microtubule associated motor proteins which consist of 2 identical (60-70 kDa) light chains and 2 identical (110-120 kDa) heavy chains, the latter of which contain a globular C-terminal tail domain, a central alpha helical rod domain and a globular N-terminal motor domain with microtubule-stimulated ATPase activity.

        Goat anti Human kinesin heavy chain antibody recognizes kinesin heavy chain as a band of 110 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
      • Target Species
        Human
      • Western Blotting
        Goat anti kinesin heavy chain detects a band of approximately 110 kDa in HEK293 cell lysates.
        Goat anti kinesin heavy chain detects a band of approximately 110 kDa in HEK293 cell lysates.
        Goat anti kinesin heavy chain detects a band of approximately 110 kDa in HEK293 cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
        Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
      • Immunogen
        Peptide sequence C-QPVAVRGGGGKQV from the C-terminal region of kinesin heavy chain.
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HEK293 lysate lyophilized in RIPA buffer.
        400μg HEK293 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        stress granule disassembly
        microtubule motor activity
        kinesin complex
        microtubule binding
        vesicle transport along microtubule
        perinuclear region of cytoplasm
        ATP binding
        microtubule
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
        STAR122P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
        STAR122P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
        STAR122P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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