Sandwich ELISA analysis of human interleukin-7 using Rat anti Human IL-7 (1012201) as a capture reagent and biotinylated Rat anti Human IL-7 (1012308) as a detection reagent with recombinant human IL-7 (PHP046) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A human serum sample is indicated (green)
Recombinant human IL-7 is 17.4 kDa protein comprised of 153 amino acids corresponing to the ful-length mature sequence.
IL-7 is a potent lymphoid cell growth factor acting on Pre-B, Pro-B and early T cells. In a dose dependent cell proliferation assay using 2E8 cells this product shows a specific activity of > 2 x 106 units/mg.
Recombinant human IL-7 is recognized by Rat anti Human IL-7 antibody clones BVD10-40F6 and BVD10-11C10 in ELISA.
Purified recombinant protein expressed in E. coli - lyophilised
Reconstitute with 0.1 ml distilled water. For extended storage the addition of 0.1% BSA is recommended. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
>98 % by SDS PAGE and HPLC
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution
Protein Molecular Weight
17.4KD (153 amino acids)
Reagents In The Kit
Preparing The Antibody
10mM Acetic Acid
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.