IL-7

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Sandwich ELISA analysis of human interleukin-7 using Rat anti Human IL-7 (1012201) as a capture reagent and biotinylated Rat anti Human IL-7 (1012308) as a detection reagent with recombinant human IL-7 (PHP046) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A human serum sample is indicated (green)

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  • Recombinant Human Interleukin-7
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP046E, FN, WBdatasheet pdfdatasheet pdf10 µg
    PHP046
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Recombinant human IL-7 is 17.4 kDa protein comprised of 153 amino acids corresponing to the ful-length mature sequence.

      IL-7 is a potent lymphoid cell growth factor acting on Pre-B, Pro-B and early T cells. In a dose dependent cell proliferation assay using 2E8 cells this product shows a specific activity of > 2 x 106 units/mg.

      Recombinant human IL-7 is recognized by Rat anti Human IL-7 antibody clones BVD10-40F6 and BVD10-11C10 in ELISA.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein expressed in E. coli - lyophilised
    • Reconstitution
      Reconstitute with 0.1 ml distilled water. For extended storage the addition of 0.1% BSA is recommended.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
    • Preservative Stabilisers
      None present
    • Purity
      >98 % by SDS PAGE and HPLC
    • Approx. Protein Concentrations
      Total protein concentration 0.1 mg/ml after reconstitution
    • Protein Molecular Weight
      17.4KD (153 amino acids)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1 ng/ug
    • Test Principle
    • Buffer Solution
      10mM Acetic Acid
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      interleukin-7 receptor binding
      positive regulation of B cell proliferation
      humoral immune response
      extracellular space
      organ morphogenesis
      positive regulation of T cell differentiation
      bone resorption
      cytokine activity
      growth factor activity
      cell-cell signaling
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4 ng/well
      Functional Assays0.1ng/ml10ng/ml
      Western Blotting1.53.0 ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      Recombinant human IL-7 may be used as a standard for ELISA applications with either anti-Human IL-7 (purified) or anti-Human IL-7 (biotin conjugate).
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Recombinant human IL-7 may be used as the positive control for western blotting applications with either anti-Human IL-7 (purified) or anti-Human IL-7 (biotin conjugate).
    • Instructions For Use

    Additional IL-7 Formats

    Formats Applications Sizes available
    IL-7 : Rec. Protein E, FN, WB 10 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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