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  • Recombinant Human Interleukin-12
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP100E, FN, WBdatasheet pdfdatasheet pdf10 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Recombinant human Interleukin-12 (IL-12) produced in CHO cells is a ~75 kDa glycoprotein comprised of disulfide-linked 35kDa (p35) and 40kDa (p40) subunits.

      IL-12 is produced by activated B lymphocytes and macrophages. The properties of IL-12 include the stimulation of growth of activated T lymphocytes, and actions in the development of Th1 CD4 +ve cells and CD8 +ve cytotoxic T cells. IL-12 also promotes activity and division of NK cells and promotes formation of TH1 cells producing &gamma-interferon.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4 ng/well
      Functional Assays0.110 ng/ml
      Western Blotting1.53.0 ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      Recombinant human IL-12 may be used as a standard for ELISA applications with either anti-human IL-12:purified (AHP779) or anti-human IL-12:biotin conjugate (AHP779B).
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Recombinant human IL-12 may be used as thepostivie control for wester blotting applications with either anti-human IL-12:purified (AHP779) or anti-human IL-12:biotin conjugate (AHP779B).
    • Instructions For Use

    Additional IL-12 Formats

    Formats Applications Sizes available
    IL-12 : Rec. Protein E, FN, WB 10 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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