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Human IgG3 | AbD18705_hIgG3

Human IgG3 | AbD18705_hIgG3 gallery image 1

ELISA analysis of recombinant Human IgG3 kappa

Recombinant Human IgG3 kappa (HCA194) detected with Mouse anti Human IgG3 (5247-9850) followed by HRP conjugated Rabbit (Fab')2 anti Mouse IgG (STAR13B)

Human IgG3 | AbD18705_hIgG3 gallery image 2

SDS-PAGE analysis of recombinant Human IgG3 kappa

Recombinant Human IgG3 kappa was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands are visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated, respectively. The molecular weights (kDa) of the marker proteins (Lane 1) are indicated on the left hand side of the image

Human IgG3 | AbD18705_hIgG3 gallery image 3

Size exclusion chromatography of recombinant Human IgG3 kappa

UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgG3 kappa. The larger peak corresponds to monomeric, fully assembled AbD18705_hIgG3. The smaller shoulder most likely contains dimers and aggregates

  • Human IgG3 | AbD18705_hIgG3 thumbnail image 1
  • Human IgG3 | AbD18705_hIgG3 thumbnail image 2
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  • Recombinant Human IgG3 Kappa
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
  • Clone
  • Isotype
    IgG3 Kappa
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA194Edatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Recombinant Human IgG3 kappa, clone AbD18705_hIgG3 is a recombinant human IgG3 antibody with a kappa light chain and a heavy chain belonging to the G3m(b1) allotype, previously designated G3m5. Clone AbD18705_hIgG3 is specific for Green Fluorescent Protein and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgG3 levels, or as a control antibody when using other human antibodies of the same isotype and subclass.

      Clone AbD18705_hIgG3 forms part of a range of human recombinant immunoglobins containing the kappa light chain. A similar range of reagents containing the lambda light chain is also available from Bio-Rad.
    • Intended Use
    • Product Form
      Human IgG3 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.01% Thiomersal
    • Immunogen
      Green Fluorescent Protein (GFP).
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      innate immune response
      antigen binding
      protein binding
      complement activation, classical pathway
      membrane fraction
      extracellular region
      immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany, a wholly owned subsidiary of Bio-Rad Laboratories Inc.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Human IgG3 Formats

    Formats Clone Applications Sizes available
    Human IgG3 : Purified AbD18705_hIgG3 E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Recombinant Human IgG3 LambdaHCA1780.1 mgE

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Human IgG3 | AbD18705_hIgG3 thumbnail image 1
                • Human IgG3 | AbD18705_hIgG3 thumbnail image 2
                • Human IgG3 | AbD18705_hIgG3 thumbnail image 3

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