IgG IgA IgM Antibody

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IgG IgA IgM Antibody gallery image 1

Published customer image:
Horseradish peroxidase conjugated Goat anti Human IgG/A/M antibody (STAR90P) used as a detection reagent for the measurement of total antibody response to tuberculoid antigens by ELISA.
Image caption:
Compared to healthy subjects, TB patients display diminution in antibody levels against PhoP but not ESAT-6, CFP-10 and Acr1.
Antibodies were measured in the serum of healthy, TB patients and close contacts against (A) PhoP; (B) Acr1; (C) ESAT-6 and (D) CFP-10. Median with 95% Cl represent the antibodies titers and each dot symbolizes single individual (N: number of individuals). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns: non-significant.

From: Vidyarthi A, Khan N, Agnihotri T, Siddiqui KF, Nair GR, Arora A, et al. (2017)
Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.
PLoS ONE 12(3): e0173769.

Enlarge
IgG IgA IgM Antibody gallery image 2

Published customer image:
Horseradish peroxidase conjugated Goat anti Human IgG/A/M antibody (STAR90P) used as a detection reagent for the measurement of total antibody response to tuberculoid antigens by ELISA.
Image caption:
PhoP showed maximum antibody titer compared to ESAT-6, CFP-10 and Acr-1.
Antibodies against PhoP, Acr1, ESAT-6 and CFP-10 were measured in the serum of (A) patients; (B) contacts; (C) healthy. Median with 95% Cl represent the antibodies titers and each dot symbolizes single individual (N: number of individuals). **p<0.01, ***p<0.001, ****p<0.0001, ns: non-significant.

From: Vidyarthi A, Khan N, Agnihotri T, Siddiqui KF, Nair GR, Arora A, et al. (2017)
Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.
PLoS ONE 12(3): e0173769.

Enlarge
IgG IgA IgM Antibody gallery image 3

Published customer image:
Horseradish peroxidase conjugated Goat anti Human IgG/A/M antibody (STAR90P) used as a detection reagent for the measurement of total antibody response to tuberculoid antigens by ELISA.
Image caption:
Ratio of Acr-1/PhoP antibody titer, discriminates among patients, contacts and healthy subjects.
Antibodies ratio of (A) Acr-1/PhoP; (B) PhoP/Acr-1 were measured using the antibody titer against PhoP and Acr-1 in the serum of healthy, TB patients and close contacts. Median with 95% Cl represent the antibodies ratio between two antigens and each dot symbolizes single individual (N: number of individuals). *p<0.05, ****p<0.0001.

From: Vidyarthi A, Khan N, Agnihotri T, Siddiqui KF, Nair GR, Arora A, et al. (2017)
Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.
PLoS ONE 12(3): e0173769.

Enlarge
IgG IgA IgM Antibody gallery image 4

Published customer image:
Horseradish peroxidase conjugated Goat anti Human IgG/A/M antibody (STAR90P) used as a detection reagent for the measurement of total antibody response to tuberculoid antigens by ELISA.
Image caption:
Relapsed cases show lower anti-PhoP antibodies than healthy volunteers.
Antibodies against PhoP, Acr-1, ESAT-6 and CFP-10 were measured in the serum of healthy individuals, patients and relapsed cases. Median with 95% Cl represent the antibodies titers and each dot symbolizes single individual (N: number of individuals). *p<0.05, ****p<0.0001, ns: non-significant.

From: Vidyarthi A, Khan N, Agnihotri T, Siddiqui KF, Nair GR, Arora A, et al. (2017)
Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.
PLoS ONE 12(3): e0173769.

Enlarge
IgG IgA IgM Antibody gallery image 5

Published customer image:
Horseradish peroxidase conjugated Goat anti Human IgG/A/M antibody (STAR90P) used as a detection reagent for the measurement of total antibody response to tuberculoid antigens by ELISA.
Image caption:
Diabetic TB patients show lower anti-ESAT-6 antibody response compared to non-diabetic TB patients.
Antibodies against PhoP, Acr-1, ESAT-6 and CFP-10 were measured in the serum of diabetic TB patients and non-diabetic TB patients. Median with 95% Cl represent the antibodies titers and each dot symbolizes single individual (N: number of individuals).

From: Vidyarthi A, Khan N, Agnihotri T, Siddiqui KF, Nair GR, Arora A, et al. (2017)
Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.
PLoS ONE 12(3): e0173769.

Enlarge
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  • Goat anti Human IgG/A/M:FITC
  • Goat anti Human IgG/A/M:HRP
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR90FE, Fdatasheet pdfdatasheet pdf2 mg
    STAR90F
    STAR90PE, WBdatasheet pdfdatasheet pdf2 mg
    STAR90P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human IgG/A/M antibody recognizes human Ig, binding to all major classes (IgG, IgA, IgM).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilised
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Reconstitute with 2ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
    • Antiserum Preparation
      Antisera to human Ig were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.01%Sodium Azide
      1%Bovine Serum Albumin
      0.01%gentamicin sulphate
      1%Bovine Serum Albumin
    • Immunogen
      Human IgG, IgA and IgM whole molecules
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 2.0 mg/ml after reconstitution
      IgG concentration 1.0 mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      antigen binding
      integral to membrane
      protein binding
      membrane fraction
      extracellular region
      immune response
      protein-chromophore linkage
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.04 ug/ml0.2 ug/ml
      Flow Cytometry0.1 ug/ml0.5 ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/50,000
      Western Blotting1/1,0001/5,000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IgG IgA IgM Antibody Formats

    Formats Applications Sizes available
    IgG IgA IgM Antibody : HRP E, WB 2 mg
    IgG IgA IgM Antibody : FITC E, F 2 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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