Human Seroblock

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Human Seroblock gallery image 1

Staining of THP-1 cells with FITC labelled Mouse anti Human CD11a (MCA1848) or Mouse IgG2a Negative Control:FITC (MCA929F) in the absence (A) and presence (B) of Human SeroBlock (BUF070A). Antibody and isotype control were both used neat. Blue shaded areas represents cells stained with the CD11a antibody while the clear areas represents cells stained with isotype control

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  • Human Seroblock
  • Human Seroblock
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF070BFdatasheet pdfdatasheet pdf200 Test
    BUF070B
    BUF070AFdatasheet pdfdatasheet pdf50 Test
    BUF070A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human Fc receptors are expressed on a variety of immune cell types including monocytes, macrophages, B cells, granulocytes and dendritic cells. Cells that express Fc receptors can give false positive immunofluorescent staining due to the Fc receptors binding of Ig. Human SeroBlock is designed to prevent such non-specific staining without interfering with appropriate target staining. Human SeroBlock is compatible with use of anti-human antibodies targeting Fc receptors in flow cytometry.
    • Intended Use
    • Target Species
      Human
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry*See Instructions For Use
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry*See Instructions For Use

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

      1) Add 5 ul of Human SeroBlock per 100ul cell suspension for 5-10 minutes at room temperature.

      2) Add test antibody according to manufacturers instructions – Do not wash Human SeroBlock off the cells. Human SeroBlock is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*Human SeroBlock is also compatible with flow cytometric analysis of human Fc receptors.

      3) Proceed with staining as usual.

    • Instructions For Use
      In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

      1) Add 5 ul of Human SeroBlock per 100ul cell suspension for 5-10 minutes at room temperature.

      2) Add test antibody according to manufacturers instructions – Do not wash Human SeroBlock off the cells. Human SeroBlock is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*Human SeroBlock is also compatible with flow cytometric analysis of human Fc receptors.

      3) Proceed with staining as usual.

    Additional Human Seroblock Formats

    Formats Applications Sizes available
    Human Seroblock : Reagent F 50 Test | 200 Test
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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