HbA1c Antibody | 15783

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HbA1c Antibody | 15783 gallery image 1

Purified HbA1c in different concentrations was spiked in hemoglobin samples (with low HbA1c content of 2.5%) and detected via HRP labeled HCA179 in ELISA. Best-fit curve shows linear correlation of fluorescence signal to HbA1c concentration

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HbA1c Antibody | 15783 gallery image 2

Data obtained from measuring the HbA1c content of serum samples using HRP labeled HCA179 antibody. Included were patient samples with abnormal hemoglobin (Hb). Besides binding to the normal glycated hemoglobin (Control 1, 2 and 3), HCA179 also recognizes the glycated hemoglobin variants HbC, HbE, HbD and to a lesser degree HbS. Elevated HbF cannot be detected due to the absence of the hemoglobin β chain. Values are given as % HbA1c. Samples were obtained from heterozygous donors. The values reflect the percentage of abnormal Hb carried by the donor.
* Data from triplicate experiments

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HbA1c Antibody | 15783 gallery image 3

Data obtained from measuring the HbA1c content of serum samples using HRP labeled HCA179 antibody. Included were patient samples with abnormal hemoglobin (Hb). Besides binding to the normal glycated hemoglobin (Control 1, 2 and 3), HCA179 also recognizes the glycated hemoglobin variants HbC, HbE, HbD and to a lesser degree HbS. Elevated HbF cannot be detected due to the absence of the hemoglobin ß chain. Values are given as mmol/mol HbA1c.
1. Samples were obtained from heterozygous donors. The values reflect the mmol/mol of abnormal Hb carried by the donor.
The mmol/mol values were calculated using the IFCC standardization formula:
NGSP = [0.09148 x IFCC] + 2.152
* Data from triplicate experiments

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  • Human anti Human HbA1c
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  • Product Type
    Monoclonal Antibody
  • Clone
    15783
  • Isotype
    HuCAL Fab monovalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA179Edatasheet pdfdatasheet pdf0.1 mg
    HCA179
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Human HbA1c antibody, clone 15783 recognizes HbA1c, i.e. hemoglobin when glycated at the N-terminal valine of the hemoglobin β chain. It does not bind to non-glycated hemoglobin. Besides binding to the normal glycated hemoglobin, it also recognizes the glycated hemoglobin variants HbC, HbE, HbD and to a lesser degree HbS. Elevated HbF cannot be detected due to the absence of the hemoglobin β chain.

      The measurement of HbA1c levels is used to identify the average plasma glucose concentration. In Diabetes mellitus, higher levels of HbA1c have been associated with cardiovascular disease and other diseases. Levels of more than 6.5% HbA1c (48 mmol/mol) are used as a criterion to diagnose diabetes.
    • Intended Use
    • Target Species
      Human
    • Product Form
      A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
    • Reconstitution
    • Preparation
      StrepTactin affinity chromatography.
    • Preservative Stabilisers
      0.01% Thiomersal
    • Immunogen
      The immunogens used in the generation of this antibody were:
      A glycated peptide, corresponding to N-terminal 9 amino acids of haemoglobin beta chain coupled to carrier proteins.
      Purified HbA1c.
    • Purity
    • Affinity
      The affinity of this antibody, as measured against the immunogen, was measured as KD=19 nM by real time, label-free molecular interaction analysis using HbA1c-BSA and HbA1c-TRF conjugates as immobilized antigen.

      The affinity of this antibody, as measured against purified HbA1c, was measured as KD=58 nM by real time, label-free molecular interaction analysis using full length HbA1c as immobilized antigen.
    • Approx. Protein Concentrations
      Total protein concentration 0.5 mg/ml.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
      This product is suitable for use in direct and indirect ELISA applications.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional HbA1c Antibody Formats

    Formats Clone Applications Sizes available
    HbA1c Antibody : Purified 15783 E 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-V5Sx2 Negative ControlHCA1250.1 mgC, E, F, P, WB
        HCA125

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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