GM-CSF

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  • Recombinant Human GM-CSF
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP235E, FN, WBdatasheet pdfdatasheet pdf20 µg
    PHP235
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • GM-CSF (granulocyte-macrophage colony-stimulating factor) is a haematopoietic growth factor which exists in both glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The ability of recombinant GM-CSF to increase haematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.2ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
    • Preparation
      Purified recombinant GM-CSF expressed in E. coli.
    • Preservative Stabilisers
      10mM Sodium citrate pH3.5
    • Activity
      The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
    • Purity
      >98% by SDS PAGE/HPLC analysis
    • Approx. Protein Concentrations
      0.1mg/ml after reconstitution
    • Molecular Weight
      14.6kD (128 amino acid residues)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <1EU/ug
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      granulocyte macrophage colony-stimulating factor receptor binding
      negative regulation of cytolysis
      cytokine activity
      positive regulation of macrophage derived foam cell differentiation
      extracellular space
      positive regulation of interleukin-23 production
      positive regulation of tyrosine phosphorylation of Stat5 protein
      positive regulation of DNA replication
      growth factor activity
      immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays1.0ng/ml
      Western Blotting1.53.0ng/lane

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      Recombinant human GM-CSF may be used as the standard in ELISA applications with either a purified human GM-CSF antibody (AHP772) or a biotinylated human GM-CSF antibody (AHP772B).
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Recombinant human GM-CSFmay be used as a positive control for Western Blotting applications with either a purified human GM-CSF antibody (AHP772) or a biotinylated human GM-CSF antibody (AHP772B).
    • Instructions For Use

    Additional GM-CSF Formats

    Formats Applications Sizes available
    GM-CSF : Rec. Protein E, FN, WB 20 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays

              References

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