GAPDH Antibody

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GAPDH Antibody gallery image 1

Western blotting analysis of lysates of cell line HEK293 (A) and HeLa (B) (35μg protein in RIPA buffer) using Goat anti Human GAPDH antibody (AHP1064) at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

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GAPDH Antibody gallery image 2

Western blotting analysis of human liver (A) and tonsil (B) lysate (35μg protein in RIPA buffer) using Goat anti Human GAPDH antibody (AHP1064) at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

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GAPDH Antibody gallery image 3

Western blotting analysis of mouse liver (A) and rat brain (B) lysate (35μg protein in RIPA buffer) using Goat anti Human GAPDH antibody (AHP1064) at 0.5μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

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GAPDH Antibody gallery image 4

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation using Goat anti Human GAPDH antibody (AHP1064) for 1hr at a concentration of 5μgml-1 followed by Alexa Fluor 488 conjugated secondary antibody at 1μgml-1, showing cytoplasmic staining (green). The nuclear stain is DAPI (blue)

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  • Goat anti Human GAPDH (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1064E, P*, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1064
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human GAPDH antibody recognizes an epitope within the C-terminal (CT) region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.

      GAPDH translocates to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death(Dastoor et al. 2001). GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Burke et al. 1996).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2000
      Western Blotting0.010.03ug/ml
      Immunohistology - Paraffin(1)0.3ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human tonsil
    • Immunofluorescence
    • Western Blotting
      AHP1064 detects a band of approximately 35kDa in human tonsil, mouse spleen and HeLa cell lysates.
    • Instructions For Use

    Additional GAPDH Antibody Formats

    Formats Applications Sizes available
    GAPDH Antibody : Purified E, P*, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human tonsil

              References

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