GAPDH Antibody | AbD22549_hIgG1

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GAPDH Antibody | AbD22549_hIgG1 gallery image 1

Western blot analysis was performed by loading 25µl Jurkat lysate (Lanes 2-7) onto an AnykD™ Criterion™ TGX Stain-Free™ gel. Precision Plus Protein™ Prestained Standards were run in lane 1. Lanes 2 & 3 and 5 & 6 were probed with Human anti GAPDH antibody, clone AbD22549_hIgG1 (HCA272) at 1.25µg/ml (lanes 2 & 5) and 0.625µg/ml (lanes 3 & 6). Lanes 2-4 were probed with HRP conjugated Tidyblot™ (STAR209P) at 1/200. Lanes 5-7 were probed with HRP conjugated Goat anti Human IgG F(ab')2 antibody (STAR126P) at 1/2500. Visualization was carried out using the Bio-Rad Chemidoc™ Touch Imaging System.

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GAPDH Antibody | AbD22549_hIgG1 gallery image 2

Western blot analysis of GAPDH IP samples IP was performed on Jurkat cell lysates using 10 µg/ml (lanes 3 & 7), 1 µg/ml (lanes 4 & 8) Human anti GAPDH antibody, clone AbD22549_hIgG1 (HCA272). 10 µg/ml Human anti HSPA4, clone AbD23625_hIgG1 (HCA273) was used in lanes 2 & 6.

7.5 µl of each IP was loaded onto an AnykD™ Criterion™ TGX Stain-Free™ gel. 25.7µl Jurkat whole cell lysate (WCL) was run in lanes 5 & 9 while Precision Plus Protein™ Prestained Standards were run in lane 1.

Rabbit anti GAPDH antibody (VPA00187) was used at 1/1000 in lanes 1-5. HRP conjugated Tidyblot™ (STAR209P) was used at 1/200 and visualized on the Bio-Rad Chemidoc™ Touch Imaging System. Arrow points to GAPDH (molecular weight 37 kDa).

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  • Human anti GAPDH
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  • Product Type
    Monoclonal Antibody
  • Clone
    AbD22549_hIgG1
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA272IP, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA272
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Human anti GAPDH antibody, clone AbD22549_hIgG1 recognizes human glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a 335 amino acid multifunctional protein. The main function of GAPDH is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). It is a major glycolytic enzyme within the cytosol and is also involved in a number of intracellular processes, including membrane fusion, microtubule bundling, phosphotransferase, DNA replication and DNA repair. It has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death.

      GAPDH has been shown to bind to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and huntingtin (Burke et al. 1996).

      Since GAPDH is constitutively expressed in almost all tissues at high levels, this antibody is recommended for use as a loading control for western blotting.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation
      Western Blotting1/501/800

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Anti GAPDH antibody detects a band of approximately 36 kDa in Jurkat cell lysate under reducing conditions
    • Instructions For Use

    Additional GAPDH Antibody Formats

    Formats Clone Applications Sizes available
    GAPDH Antibody : Purified AbD22549_hIgG1 IP, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
          STAR126P
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

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