Western blot analysis of GAPDH IP samples IP was performed on Jurkat cell lysates using 10 µg/ml (lanes 3 & 7), 1 µg/ml (lanes 4 & 8) Human anti GAPDH antibody, clone AbD22549_hIgG1 (HCA272). 10 µg/ml Human anti HSPA4, clone AbD23625_hIgG1 (HCA273) was used in lanes 2 & 6.
Human anti GAPDH antibody, clone AbD22549_hIgG1 recognizes human glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a 335 amino acid multifunctional protein. The main function of GAPDH is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). It is a major glycolytic enzyme within the cytosol and is also involved in a number of intracellular processes, including membrane fusion, microtubule bundling, phosphotransferase, DNA replication and DNA repair. It has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death.
GAPDH has been shown to bind to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and huntingtin (Burke et al. 1996).
Since GAPDH is constitutively expressed in almost all tissues at high levels, this antibody is recommended for use as a loading control for western blotting.
Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line - liquid
Purified IgG prepared by affinity chromatography on Protein A
Recombinant human GAPDH
The monovalent intrinsic affinity of AbD22549 was measured as KD = 2 nM by real time, label free molecular interaction analysis on immobilized HSPA4
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.