Defensin Beta 2 Antibody

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Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-1 (AHP849)

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Defensin Beta 2 Antibody gallery image 2

Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-1 (AHP849)

Enlarge
Defensin Beta 2 Antibody gallery image 3

Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-1 (AHP849)

Enlarge
Defensin Beta 2 Antibody gallery image 4

Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-1 (AHP849)

Enlarge
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  • Goat anti Human Defensin Beta 2
  • Goat anti Human Defensin Beta 2:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP849E, FN, P, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP849
    AHP849BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP849B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Human Defensin beta 2 polyclonal antibody recognizes human Defensin beta-2 (BD-2), a ~4.3kDa cationic antimicrobial peptide, also known as Beta-defensin 4A or Skin-antimicrobial peptide 1 (SAP-1) expressed primarily by epithelial cells of the respiratory tract and skin, and an important component of the innate immune response against microbial infections.

      Expression of BD-2 is induced during inflammation in response to bacterial products and cytokines, and is initially expressed in a precursor form, which is cleaved to release the C-Terminal active portion of the protein which is secreted by the neutrophil and binds to bacterial membranes causing their disruption.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - lyophilised
      Purified IgG conjugated to Biotin - lyophilised
    • Reconstitution
      Reconstitute with 1.0ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      N.B. For functional studies do not add sodium azide
      Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
      Antisera to human Defensin beta 2 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
      Antisera to human Defensin beta 2 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml after reconstitution
      IgG concentration 0.1 mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      Following reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      Following reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      chemotaxis
      immune response
      extracellular region
      G-protein coupled receptor protein signaling pathway
      defense response to bacterium
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays5.08.0ug/ml
      Immunohistology - Paraffin
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0 ug/ml
      Western Blotting0.10.2 ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This purified human BD-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human BD-2 antibody (AHP849B) as the detection reagent and recombinant human BD-2 (PHP161) as the standard
    • ELISA
      This biotinylated human DB-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified human BD-2 antibody (AHP849) as the capture reagent and recombinant human BD-2 (PHP161) as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      This purified human BD-2 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human BD-2 (PHP161) as the positive control.
    • Western Blotting
      This biotinylated human BD-2 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human BD-2 (PHP161) as the positive control.
    • Instructions For Use
    • Instructions For Use

    Additional Defensin Beta 2 Antibody Formats

    Formats Applications Sizes available
    Defensin Beta 2 Antibody : Purified E, FN, P, WB 0.1 mg
    Defensin Beta 2 Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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