Mouse anti human cytokeratin 7 antibody, clone LP5K reacts with cytokeratin 7, found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and the larger collecting ducts of the kidney. Staining of blood vessel cell walls, particularly in endothelial cells, by the mouse anti human cytokeratin 7 antibody, may occasionally be observed.
Purified IgG from tissue culture supernatant prepared by ammonium sulphate precipitation followed by ion exchange chromatography then conjugated to FITC
0.1% Sodium Azide (NaN3) 0.1% Bovine Serum Albumin
Cytoskeletal proteins from cultured HeLa cells.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.