Cytokeratin 18/19 Antibody (PrecisionAbTM Antibody)

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Cytokeratin 18/19 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with cytokeratin 18/19 antibody followed by detection with HRP conjugated Goat anti Human IgG F(ab')2 (1/2500, STAR126P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to cytokeratin 18/19 (molecular weight 48, 44 kDa)

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  • Cytokeratin 18/19 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Human anti Cytokeratin 18/19
  • Cytokeratin 18/19 Antibody with Control Lysate
  • Cytokeratin 18/19 Antibody with Control Lysate
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    Cytokeratin 18/19 antibody recognizes the keratin, type I cytoskeletal 18, also known as cell proliferation-inducing gene 46, protein cytokeratin-18, CK-18, keratin-18 or K18. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      HuCAL Fab bivalent
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00011WBdatasheet pdfdatasheet pdf100 µl
      VMA00011
      VMA00011KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00011K
      VMA00011KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00011KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Human anti Human cytokeratin 18/19 antibody recognizes human cytokeratin 18, a amino acid type I intermediate filament protein expressed by epithelial cells. The intermediate filaments are expressed as heterodimers in epithelial tissues. Cytokeratin 18 (CK18) is often co-expressed with cytokeratin 8 (CK8). The CK18/CK8 heterodimer is the major cytokeratin pair found in simple epithelia such as that found in the liver, intestine and breast ducts. CK18 plays an important role in maintaining epithelial cell integrity.

        This clone has also been demonstrated to cross-react with cytokeratin 19. Human anti Human cytokeratin 18/19 antibody recognizes cytokeratin 18 as a band of ~48 kDa and cytokeratin 19 as a band of ~44 kDa in western blotting under reducing conditions.
      • Target Species
        Human
      • Western Blotting
        Anti cytokeratin 18/19 antibody detects bands of approximately 44 kDa in HeLa cell lysates.
        Anti cytokeratin 18/19 antibody detects bands of approximately 44 kDa in HeLa cell lysates.
        Anti cytokeratin 18/19 antibody detects bands of approximately 44 kDa in HeLa cell lysates.
      • Product Form
        A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
        A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
        A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
      • Preparation
        Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
        Pack Size: 10 Westerns100μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
        Pack Size: 2 Westerns20μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
        Pack Size: 10 Westerns100μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
        Pack Size: 2 Westerns20μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Recombinant human cytokeratin 18
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HeLa lysate lyophilized in RIPA buffer.
        400μg HeLa lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        interspecies interaction between organisms
        perinuclear region of cytoplasm
        cell cycle
        Golgi to plasma membrane CFTR protein transport
        protein binding
        structural molecule activity
        keratin filament
        anatomical structure morphogenesis
        negative regulation of apoptosis
        centriolar satellite
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P
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        MCA5995P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
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        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
        MCA5995P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
        MCA5995P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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