CSK Antibody

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CSK Antibody gallery image 1

Immunohistochemical analysis of CSK expression in human colon using Goat anti Human CSK antibody (AHP733) on formalin fixed, paraffin embedded tissue sections following antigen retrievalwith citrate buffer pH6.0

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CSK Antibody gallery image 2

Western blotting analysis of CSK expression in a Jurkat cell lysate using Goat anti Human CSK antibody (AHP733) at 0.5μgml-1

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CSK Antibody gallery image 3

Western blotting analysis of CSK expression in a mouse spleen lysate using Goat anti Human CSK antibody (AHP733) at 1μgml-1

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  • Goat anti Human CSK (C-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP733E, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP733
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Human CSK antibody recognizes an epitope within the C-terminal (CT) region of CSK, also known as c-src tyrosine kinase, Tyrosine-protein kinase CSK, p50CSK or Protein-tyrosine kinase CYL. CSK is a cytoplasmic tyrosine kinase ubiquitously expressed as two mRNA species (Partanen et al. 1991), which down-regulates the activity of the c-src oncoprotein (Chong et al. 2005).

      CSK is expressed in lung tissues and by macrophages and is highly conserved across species suggesting a role in important regulatory functions (Bräuninger et al. 1992).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human CSK (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
      0.5%Bovine Serum Albumin
    • Immunogen
      Peptide sequence EQLEHIKTHELHL corresponding to the C-terminal region of CSK (NP_004374.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      epidermal growth factor receptor signaling pathway
      ATP binding
      protein phosphorylation
      non-membrane spanning protein tyrosine kinase activity
      centrosome
      Golgi apparatus
      blood coagulation
      T cell receptor signaling pathway
      protein C-terminus binding
      T cell costimulation
      cytosol
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA>1/32,000
      Western Blotting0.5ug/ml2.0ug/ml
      Immunohistology - Paraffin(1)0.1ug/ml0.3ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
      TRIS/EDTA buffer pH 9.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human Tonsil
    • Immunofluorescence
    • Western Blotting
      AHP733 detects a band of approximately 50kDa in Jurkat cell lysates.
    • Instructions For Use

    Additional CSK Antibody Formats

    Formats Applications Sizes available
    CSK Antibody : Purified E, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human Tonsil

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