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CSK Antibody

CSK Antibody gallery image 1

Immunohistochemical analysis of CSK expression in human colon using Goat anti Human CSK antibody (AHP733) on formalin fixed, paraffin embedded tissue sections following antigen retrievalwith citrate buffer pH6.0

CSK Antibody gallery image 2

Western blotting analysis of CSK expression in a Jurkat cell lysate using Goat anti Human CSK antibody (AHP733) at 0.5μgml-1

CSK Antibody gallery image 3

Western blotting analysis of CSK expression in a mouse spleen lysate using Goat anti Human CSK antibody (AHP733) at 1μgml-1

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  • Goat anti Human CSK (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP733E, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human CSK antibody recognizes an epitope within the C-terminal (CT) region of CSK, also known as c-src tyrosine kinase, Tyrosine-protein kinase CSK, p50CSK or Protein-tyrosine kinase CYL. CSK is a cytoplasmic tyrosine kinase ubiquitously expressed as two mRNA species (Partanen et al. 1991), which down-regulates the activity of the c-src oncoprotein (Chong et al. 2005).

      CSK is expressed in lung tissues and by macrophages and is highly conserved across species suggesting a role in important regulatory functions (Bräuninger et al. 1992).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human CSK (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
      0.5%Bovine Serum Albumin
    • Immunogen
      Peptide sequence EQLEHIKTHELHL corresponding to the C-terminal region of CSK (NP_004374.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      epidermal growth factor receptor signaling pathway
      ATP binding
      protein phosphorylation
      non-membrane spanning protein tyrosine kinase activity
      Golgi apparatus
      blood coagulation
      T cell receptor signaling pathway
      protein C-terminus binding
      T cell costimulation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.5ug/ml2.0ug/ml
      Immunohistology - Paraffin(1)0.1ug/ml0.3ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
      TRIS/EDTA buffer pH 9.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human Tonsil
    • Immunofluorescence
    • Western Blotting
      AHP733 detects a band of approximately 50kDa in Jurkat cell lysates.
    • Instructions For Use

    Additional CSK Antibody Formats

    Formats Applications Sizes available
    CSK Antibody : Purified E, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Histar Detection SystemSTAR3000B150 TestsC, P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Human Tonsil
                • Product Images

                • CSK Antibody thumbnail image 1
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