CDK5 Antibody (PrecisionAbTM Antibody) | AB04/3H6

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CDK5 Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB04/3H6 gallery image 1

Western blot analysis of whole cell lysates probed with CDK5 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 37 second exposure. Arrow points to CDK5 (molecular weight 33 kDa)

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  • CDK5 Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB04/3H6 thumbnail image 1
  • Mouse anti CDK5
  • CDK5 Antibody with Control Lysate
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  • Product Type
    PrecisionAb™ Monoclonal
  • Isotype
    IgG1
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VMA00541WBdatasheet pdfdatasheet pdf100 µl
    VMA00541
    VMA00541KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VMA00541KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
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    • Mouse anti Human CDK5 antibody recognizes the cyclin-dependent kinase 5, also known as TPKII catalytic subunit, cell division protein kinase 5, protein kinase CDK5 splicing or tau protein kinase II catalytic subunit.

      Mouse anti Human CDK5 antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Anti CDK5 detects a band of approximately 33 kDa in Jurkat cell lysates
      Anti CDK5 detects a bands of approximately 33 kDa in Jurkat cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
      20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      E. coli-derived recombinant human CDK5 (amino acids 1 - 292)
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg Jurkat lysate lyophilized in RIPA buffer
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      embryo development
      cell proliferation
      ATP binding
      lamellipodium
      growth cone
      neuromuscular junction
      blood coagulation
      membrane
      acetylcholine receptor activator activity
      ErbB-2 class receptor binding
      dendrite
      positive regulation of neuron apoptosis
      neuronal cell body
      axon
      tau-protein kinase activity
      cytosol
      ErbB-3 class receptor binding
      cell division
      cyclin-dependent protein kinase activity
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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