CD282 Antibody | TLR2.3

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CD282 Antibody | TLR2.3 gallery image 1

Staining of human peripheral blood monocytes probed with Mouse anti Human CD282: RPE (MCA2152PE)

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CD282 Antibody | TLR2.3 gallery image 2

Staining of human peripheral blood monocytes probed with Mouse anti Human CD282 (MCA2152)

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CD282 Antibody | TLR2.3 gallery image 3

Staining of human peripheral blood monocytes probed with Mouse anti Human CD282: FITC (MCA2152F)

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CD282 Antibody | TLR2.3 gallery image 4

Staining of human peripheral blood monocytes probed with Mouse anti Human CD282: Alexa Fluor® 647 (MCA2152A647)

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  • CD282 Antibody | TLR2.3 thumbnail image 1
  • CD282 Antibody | TLR2.3 thumbnail image 2
  • CD282 Antibody | TLR2.3 thumbnail image 3
  • CD282 Antibody | TLR2.3 thumbnail image 4
  • Mouse anti Human CD282:RPE
  • Mouse anti Human CD282
  • Mouse anti Human CD282:FITC
  • Mouse anti Human CD282:Alexa Fluor® 647
  • Mouse anti Human CD282:Alexa Fluor® 647
  • Mouse anti Human CD282:FITC
  • Mouse anti Human CD282
  • Mouse anti Human CD282
  • Mouse anti Human CD282:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    TLR2.3
  • Isotype
    IgG2a
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2152FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2152F
    MCA2152GAC, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA2152GA
    MCA2152C, Fdatasheet pdfdatasheet pdf0.2 mg
    MCA2152
    MCA2152PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2152PE
    MCA2152A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2152A647
    MCA2152FTFdatasheet pdfdatasheet pdf25 µg
    MCA2152FT
    MCA2152TC, Fdatasheet pdfdatasheet pdf25 µg
    MCA2152T
    MCA2152PETFdatasheet pdfdatasheet pdf25 Tests
    MCA2152PET
    MCA2152A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2152A647T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD282 antibody, clone TL2.3 recognizes human TLR2, otherwise known as CD282. TLR2 is a member of the Toll-like receptor (TLR) family and is expressed primarily by peripheral blood monocytes.

      TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity. TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2.

      Mouse anti Human CD282 antibody, clone TL2.1 is reported to block TLR2 function.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      CHO cell line transfected with human TLR2 (CD282).
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.1 mgIgG concentration 0.5mg/ml
      Pack Size: 25 µg, 0.2 mgIgG concentration 0.5 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
      Pack Size: 0.1 mgIgG concentration 0.5mg/ml
      Pack Size: 25 µg, 0.2 mgIgG concentration 0.5 mg/ml
      Pack Size: 0.1 mgIgG concentration 0.5mg/ml
      Pack Size: 25 µg, 0.2 mgIgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the NS0 myeloma cell line.
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
      positive regulation of tumor necrosis factor production
      positive regulation of chemokine production
      toll-like receptor 4 signaling pathway
      positive regulation of Wnt receptor signaling pathway
      positive regulation of NF-kappaB transcription factor activity
      Gram-positive bacterial cell surface binding
      cytoplasm
      Toll signaling pathway
      positive regulation of interleukin-18 production
      positive regulation of interleukin-8 production
      positive regulation of interferon-beta production
      detection of triacyl bacterial lipopeptide
      peptidoglycan binding
      MyD88-dependent toll-like receptor signaling pathway
      detection of diacyl bacterial lipopeptide
      triacyl lipopeptide binding
      positive regulation of NF-kappaB import into nucleus
      positive regulation of inflammatory response
      lipopolysaccharide receptor activity
      I-kappaB phosphorylation
      inflammatory response
      positive regulation of nitric-oxide synthase biosynthetic process
      toll-like receptor 2 signaling pathway
      induction of apoptosis
      cellular response to diacyl bacterial lipopeptide
      toll-like receptor 1 signaling pathway
      positive regulation of interleukin-12 production
      integral to plasma membrane
      innate immune response
      transmembrane receptor activity
      positive regulation of gene-specific transcription from RNA polymerase II promoter
      positive regulation of interleukin-6 production
      cellular response to triacyl bacterial lipopeptide
      protein heterodimerization activity
      cellular response to lipoteichoic acid
      Toll-like receptor 1-Toll-like receptor 2 protein complex
      positive regulation of toll-like receptor signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Tonsil.
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Tonsil
    • Histology Positive Control Tissue
      Tonsil
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD282 Antibody Formats

    Formats Clone Applications Sizes available
    CD282 Antibody : Alexa Fluor® 647 TLR2.3 F 25 Tests/0.25ml | 100 Tests/1ml
    CD282 Antibody : FITC TLR2.3 F 25 µg | 0.1 mg
    CD282 Antibody : RPE TLR2.3 F 100 Tests | 25 Tests
    CD282 Antibody : Purified TLR2.3 C, F 25 µg | 0.2 mg | 0.1 mg
    • Copyright © 2016 Bio-Rad

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              Tonsil.
              Tonsil
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              • Application NameReference Images
                Flow Cytometry

              References

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