CD23 Antibody | D3.6

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CD23 Antibody | D3.6 gallery image 1

Staining of human peripheral blood lymphocytes probed with Mouse anti Human CD23:RPE (MCA1931PE)

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CD23 Antibody | D3.6 gallery image 2

Staining of human peripheral blood lymphocytes with Mouse anti Human CD23:Alexa Fluor® 647 (MCA1931A647)

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CD23 Antibody | D3.6 gallery image 3

Staining of human peripheral blood lymphocytes with Mouse anti Human CD23:FITC (MCA1931F)

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  • Mouse anti Human CD23:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    D3.6
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1931FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1931F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD23 antibody, clone D3.6 recognizes CD23 also known as FcƐRII (Fc epsilon Receptor II). CD23 is a ~45 kDa cell surface glycoprotein that acts as a low affinity receptor for IgE.

      CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells. CD23 is also expressed by eosinophils.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Immunoprecipitated CD23 antigen from purified plasma membranes from EBV-transformed human lymphoblastoid B cell lines
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      extracellular region
      integrin binding
      sugar binding
      receptor activity
      positive regulation of killing of cells of other organism
      positive regulation of nitric-oxide synthase 2 biosynthetic process
      integral to plasma membrane
      positive regulation of nitric-oxide synthase activity
      IgE binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD23 Antibody Formats

    Formats Clone Applications Sizes available
    CD23 Antibody : FITC D3.6 F 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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