CD127 Antibody | 11590

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CD127 Antibody | 11590 gallery image 1

Human peripheral blood lymphocytes stained with Human anti Human CD127 (HCA144) followed by Mouse anti Strep-tag (MCA2488) and FITC conjugated Rabbit F(ab')2 anti Mouse IgG (STAR9B)

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CD127 Antibody | 11590 gallery image 2

Acetone fixed frozen human tonsil stained with Human anti Human CD127 (HCA144) followed by Mouse anti Strep-tag (MCA2488) and Histar detection (STAR3000A)

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  • CD127 Antibody | 11590 thumbnail image 1
  • CD127 Antibody | 11590 thumbnail image 2
  • Human anti Human CD127
  • Human anti Human CD127
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    11590
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA144C, F*datasheet pdfdatasheet pdf0.1 mg
    HCA144
    HCA144AC, F*datasheet pdfdatasheet pdf50 µg
    HCA144A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Human CD127 antibody, clone 11590 recognizes the alpha chain of the human interleukin-7 receptor (IL-7R), which is also known as CD127. CD127 is a ~65-75 kDa transmembrane glycoprotein that associates with the interleukin-2 receptor gamma chain to form the functional high affinity IL-7 receptor. CD127 is expressed on lymphoid precursors in the bone marrow, pro-B cells, a subpopulation of thymocytes, T-cells and monocytes. CD127 is the specific receptor for IL-7 and the IL-7 receptor plays a critical role in lymphoid development.
    • Intended Use
    • Target Species
      Human
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a DYKDDDDK tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a DYKDDDDK tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Recombinant Human IL-7 R alpha/CD127/Fc Chimera.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting
      Flow Cytometry(1)1/101/200
      (1)
      HCA144 has been demonstrated to function in Flow Cytometry, however Bio-Rad recommend the use of clone 11592 (HCA145) which demonstrates stronger staining.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting
      Flow Cytometry(1)1/101/200
      (1)
      HCA144 has been demonstrated to function in Flow Cytometry, however Bio-Rad recommend the use of clone 11592 (HCA145) which demonstrates stronger staining.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • ELISA
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human tonsil
    • Histology Positive Control Tissue
      Human tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD127 Antibody Formats

    Formats Clone Applications Sizes available
    CD127 Antibody : Purified 11590 C, F* 50 µg | 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti DYKDDDDK Tag:HRPAHP1074P0.1 mgE, WB
      AHP1074P
      Mouse anti Strep-Tag ImmoMCA24880.1 mgE
      MCA2488
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti DYKDDDDK Tag:HRPAHP1074P0.1 mgE, WB
      AHP1074P
      Mouse anti Strep-Tag ImmoMCA24880.1 mgE
      MCA2488

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-FSx2 Negative ControlHCA104A50 µgC, E, F, P, WB
        HCA104A

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B

          Recommended Positive Controls

            Histology Controls

              Human tonsil
              Human tonsil

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