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C-Reactive Protein
ELISA analysis of C-reactive protein using Mouse anti C-reactive protein (MCA5880) as a capture reagent and biotinylated Mouse anti C-reactive protein (MCA5882G) as a detection reagent with native human C-reactive protein (PHP277) as antigen. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum sample is displayed at 125- (Green), 250- (Red) , and 500- (Blue) fold dilutions
Enlarge- Native Human C-Reactive Protein
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Product TypePurified Protein
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- C-reactive protein is an acute phase protein, produced by the liver that is used as a marker of inflammation and for monitoring and predicting coronary artery disease. It exists in the blood as a large pentamer of approximately 114 kDa.
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Intended Use
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Target SpeciesHuman
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Product FormPurified protein - liquid
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Reconstitution
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Preparation0.2 µM sterile filtered
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Preservative Stabilisers0.09% Sodium Azide (NaN3)
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Purity>99%
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Approx. Protein Concentrations1.0 mg/ml
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Molecular Weight114 kDa
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionTris buffered saline, 5 mM CaCl2.
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StorageStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life12 months from date of despatch.
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GO Termsnegative regulation of macrophage derived foam cell differentiationcholine bindingprotein bindingopsonizationnegative regulation of lipid storagelow-density lipoprotein particle bindingmetal ion bindingGram-positive bacterial cell surface bindingacute-phase response
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UniProt
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Entrez Gene
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA - Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Technical Advice
- Recommended Protocol
- ELISA
- Immunohistology
- Histology Positive Control Tissue
- Immunofluorescence
- Western Blotting
- Instructions For Use
Additional C-Reactive Protein Formats
| Formats | Applications | Sizes available |
|---|---|---|
| C-Reactive Protein : Purified | E | 1 mg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Negative Isotype Control
Useful Reagents
Recommended Positive Controls
Histology Controls
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Formerly known as AbD Serotec, a global supplier and manufacturer of antibodies, kits, and reagents.
Copyright © 2018 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2018 Bio-Rad Laboratories, Inc. All rights reserved
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