ELISA analysis of C-reactive protein using Mouse anti C-reactive protein (MCA5880) as a capture reagent and biotinylated Mouse anti C-reactive protein (MCA5882G) as a detection reagent with native human C-reactive protein (PHP277) as antigen. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum sample is displayed at 125- (Green), 250- (Red) , and 500- (Blue) fold dilutions
C-reactive protein is an acute phase protein, produced by the liver that is used as a marker of inflammation and for monitoring and predicting coronary artery disease. It exists in the blood as a large pentamer of approximately 114 kDa.
Purified protein - liquid
0.2 µM sterile filtered
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Tris buffered saline, 5 mM CaCl2.
Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.