Aurora-B Kinase Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-aurora-b-kinase-antibody-vpa00485.html?RenderPageSpecific=1
Aurora-B Kinase Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with aurora-B kinase antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 139 second exposure. Arrow points to aurora-B kinase (molecular weight 48 kDa)

Enlarge
  • Aurora-B Kinase Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Aurora-B Kinase Antibody with Control Lysate
  • Rabbit anti Aurora-B Kinase
(Rated 0.0 out of 5 based on 0 customer reviews)
    Rabbit anti-human Aurora-B kinase antibody recognizes Aurora kinase B, also known as ARK-2, STK-1, aurora 1, aurora- and Ipl1-like midbody-associated protein 1, protein phosphatase 1, regulatory subunit 48, serine/threonine kinase 12 or serine/threonine-protein kinase 5. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00485WBdatasheet pdfdatasheet pdf100 µl
      VPA00485
      VPA00485KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00485KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human aurora-B kinase antibody recognizes aurora B-kinase, also known as ARK-2, STK-1, aurora 1, aurora kinase B-Sv1, aurora kinase B-Sv2, aurora- and Ipl1-like midbody-associated protein 1, aurora-related kinase 2, aurora/IPL1-related kinase 2, serine/threonine-protein kinase 12 or serine/threonine-protein kinase B.

        Encoded by the AURKB gene, aurora-B kinase is a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of AURKB is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for AURKB (provided by RefSeq, Jan 2013).

        Rabbit anti Human aurora-B kinase antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti aurora-B kinase detects a band of approximately 48 kDa in HEK293 cell lysates
        Anti aurora-B kinase detects a band of approximately 48 kDa in HEK293 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Rabbit Ig fraction prepared by ammonium sulphate precipitation
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        KLH conjugated synthetic peptide corresponding to amino acid 283-313 of human aurora-B kinase
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HEK293 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        condensed nuclear chromosome, centromeric region
        protein localization to kinetochore
        chromosome passenger complex
        spindle
        cytosol
        ATP binding
        protein binding
        protein phosphorylation
        anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
        mitotic prometaphase
        metal ion binding
        protein serine/threonine kinase activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted