Western blotting analysis of human placental lysate (35μg protein in RIPA buffer) probed with Goat anti Human Apolipoprotein L1 antibody (AHP1896) at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Goat anti Human apolipoprotein L1 antibody recognises an epitope within the C-Terminal (CT) region of human Apolipoprotein L1 (APOL1), a secreted high density lipoprotein and member of the apolipoprotein L family, which interacts with apolipoprotein A-I (APOA1) in plasma.
Two isoforms of APOL1 have been identified, which may play a role in lipid exchange and transport throughout the body, and possibly in the reverse transport of cholesterol from peripheral cells to the liver.
Goat anti Human apolipoprotein L1 is expected to recognise both reported isoforms of human APOL1.
Purified IgG - liquid
Antiserum to human APOL1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
0.02% Sodium Azide (NaN3) 0.5% Bovine Serum Albumin
Synthetic peptide C-NNNYKILQADQE from within the C-Terminal region of human APOL1 (NP_003652.2; NP_663318.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP1896 detects a band of approximately 45kDa in human brain (frontal cortex) cell lysates