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AIF1 antibody

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Anti AIF1 (C-Terminal) Antibody thumbnail image 1 Anti AIF1 (C-Terminal) Antibody thumbnail image 2 Anti AIF1 (C-Terminal) Antibody thumbnail image 3 Anti AIF1 (C-Terminal) Antibody thumbnail image 4 Anti AIF1 (C-Terminal) Antibody thumbnail image 5 Anti AIF1 (C-Terminal) Antibody thumbnail image 6
Anti AIF1 (C-Terminal) Antibody gallery image 1
Immunohistochemical staining of formalin fixed, paraffin embedded mouse brain with Goat anti Human AIF1 antibody (AHP2024) following heat mediated antigen retrieval in Tris/EDTA buffer (pH9.0)
Anti AIF1 (C-Terminal) Antibody gallery image 2
Western blotting of Human (A) Mouse (B) and Rat (C) Brain lysate (35µg protein in RIPA buffer) probed with Goat anti Human AIF1 antibody (AHP2024) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Anti AIF1 (C-Terminal) Antibody gallery image 3
Western blot analysis of rat brain lysate probed with Goat anti Human AIF1 antibody (AHP2024) followed by HRP conjugated anti Goat antibody and detection by chemiluminescence.
Anti AIF1 (C-Terminal) Antibody gallery image 4
Published customer image:
Goat anti AIF1 antibody (AHP2024) used to label microglial cells in rat hippocampus by immunofluorescence.
Image caption:
Cerebrolysin decreases the density of glial cells after pilocarpine-induced seizure. The administration of cerebrolysin decreased the density of glial cells after pilocarpine-induced seizure. (A,D) Iba-1 (red), GFAP (green), and DAPI (blue) in the hippocampal CA1 (A) and CA3 (D) regions. The administration of cerebrolysin after a seizure decreased the density of glial cells in the hippocampal CA1 and CA3 regions compared to the seizure-vehicle groups. Scale bar = 20 μm. (B,C,E,F) A graph of the density of glial cells according to the standard. The data are the mean ± SEM, n = 5, from each sham group; n = 5–7 for each seizure group. *p <0.05 vs. vehicle-treated group; #p <0.05 vs. sham-operated group [Kruskal–Wallis test with post hoc test: (B) Chi square = 17.244, df = 3, p = 0.001, (C) Chi square = 17.153, df = 3, p <0.001, (E) Chi square = 17.456, df = 3, p = 0.001, (F) Chi square = 18.292, df = 3, p < 0.001].

From: Kang DH, Choi BY, Lee SH, Kho AR, Jeong JH, Hong DK, Kang BS, Park MK, Song HK, Choi HC, Lim MS, Suh SW.
Effects of Cerebrolysin on Hippocampal Neuronal Death After Pilocarpine-Induced Seizure.
Front Neurosci. 2020 Oct 16;14:568813.
doi: 10.3389/fnins.2020.568813.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti AIF1 (C-Terminal) Antibody gallery image 5
Published customer image:
Goat anti AIF1 antibody (AHP2024) used to label microglial cells in rat hippocampus by immunofluorescence.
Image caption:
Cerebrolysin decreases the density of glial cells after pilocarpine-induced seizure. The administration of cerebrolysin decreased the density of glial cells after pilocarpine-induced seizure. (A,D) Iba-1 (red), GFAP (green), and DAPI (blue) in the hippocampal CA1 (A) and CA3 (D) regions. The administration of cerebrolysin after a seizure decreased the density of glial cells in the hippocampal CA1 and CA3 regions compared to the seizure-vehicle groups. Scale bar = 20 μm. (B,C,E,F) A graph of the density of glial cells according to the standard. The data are the mean ± SEM, n = 5, from each sham group; n = 5–7 for each seizure group. *p <0.05 vs. vehicle-treated group; #p <0.05 vs. sham-operated group [Kruskal–Wallis test with post hoc test: (B) Chi square = 17.244, df = 3, p = 0.001, (C) Chi square = 17.153, df = 3, p <0.001, (E) Chi square = 17.456, df = 3, p = 0.001, (F) Chi square = 18.292, df = 3, p < 0.001].

From: Kang DH, Choi BY, Lee SH, Kho AR, Jeong JH, Hong DK, Kang BS, Park MK, Song HK, Choi HC, Lim MS, Suh SW.
Effects of Cerebrolysin on Hippocampal Neuronal Death After Pilocarpine-Induced Seizure.
Front Neurosci. 2020 Oct 16;14:568813.
doi: 10.3389/fnins.2020.568813.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti AIF1 (C-Terminal) Antibody gallery image 6
Published customer image:
Goat anti AIF1 antibody (AHP2024) used to label murine microglia by immunofluorescence
Image caption:
Aryl hydrocarbon receptor deficiency (AhRKO) after thrombin injection in vivo increased MMP9 activity, but not MMP2. (C) Representative images of brain sections at 72 h after thrombin injection, showing double staining with both microglia cell marker Iba1 (green) and MMP 9 (red). Scale bars: 50 μm.

From: Sheu ML, Pan LY, Yang CN, Sheehan J, Pan LY, You WC, Wang CC, Pan HC.
Thrombin-Induced Microglia Activation Modulated through Aryl Hydrocarbon Receptors.
Int J Mol Sci. 2023 Jul 13;24(14):11416.
doi: 10.3390/ijms241411416.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Goat anti AIF1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
AIF1
Region
(C-TERMINAL)

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Product Code Applications Pack Size List Price Your Price Qty
AHP2024
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.1 mg loader
List Price Your Price
loader

Goat anti AIF1 antibody recognizes human allograft inflammatory factor 1 (AIF1), otherwise known as Ionized calcium-binding adapter molecule 1 (IBA1). AIF1 is a 147 amino acid ~17kDa actin-binding protein involved in macrophage activation, vascular smooth muscle cell proliferation and activation and T-lymphocyte proliferation (UniProt P55008). Goat anti AIF1 antibody recognizes epitopes at the C-terminal region of AIF1 and is expected to recognize AIF1 isoform 1 and isoform 3 formed by alternative splicing.

AIF1 was first identified in rat cardiac allografts with chronic rejection (Utans et al. 1995), is expressed by activated monocytes and macrophages (Orsmark et al. 2007) and plays a significant role in vascular inflammation (Chen et al. 2004)

Goat anti AIF1 has been used successfully for the identification of AIF1 expressing cells in an inflammatory model in formalin fixed mouse brain following heat mediated antigen retrieval with sodium citrate buffer by immunohistochemistry (Wang et al. 2014).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Pig Expected from Sequence
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human AIF1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-TGPPAKKAISELP from the C-terminal region of AIF1 (NP_116573.1; NP_001614.3).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/32000
Immunohistology - Paraffin 1 2.0ug/ml
Western Blotting 1.0 3.0ug/ml
  1. 1This antibody requires heat-mediated antigen retrieval prior to staining paraffin sections. Tris/EDTA buffer pH9.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2024 detects a band of approximately 16kDa in rat brain cell lysates.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
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loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0

References for AIF1 antibody

  1. Kang, D.H. et al. (2020) Effects of Cerebrolysin on Hippocampal Neuronal Death After Pilocarpine-Induced Seizure.
    Front Neurosci. 14: 568813.
  2. Wu, C. et al. (2022) E3 ubiquitin ligase Triad1 promotes neuronal apoptosis by regulating the p53-caspase3 pathway after spinal cord injury.
    Somatosens Mot Res. 39 (1): 21-8.
  3. Zhou, C. et al. (2022) Mild Hypothermia Protects Brain Injury After Intracerebral Hemorrhage in Mice Via Enhancing the Nrdp1/MyD88 Signaling Pathway.
    Neurotox Res. 40 (6): 1664-72.
  4. Sheu, M.L. et al. (2023) Thrombin-Induced Microglia Activation Modulated through Aryl Hydrocarbon Receptors.
    Int J Mol Sci.24 (14):11416.
  5. Lu, X. et al. (2018) HAX1 is associated with neuronal apoptosis and astrocyte proliferation after spinal cord injury.
    Tissue Cell. 54: 1-9.
  6. Yu, M. et al. (2023) PU.1 interaction with p50 promotes microglial-mediated inflammation in secondary spinal cord injury in SCI rats.
    Int J Neurosci. 133 (4): 389-402.

Synonyms
IBA1
RRID
AB_2224406
UniProt
P55008
P55009
Entrez Gene
AIF1
Aif1
GO Terms
GO:0005509 calcium ion binding
GO:0051015 actin filament binding
GO:0051017 actin filament bundle assembly
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0006954 inflammatory response
GO:0007050 cell cycle arrest
GO:0008285 negative regulation of cell proliferation
GO:0032587 ruffle membrane

AHP2024

159698 170421

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