Optimize your assays using recombinant antibodies selected for desired affinity
Boschert et al., (2016) The sclerostin-neutralizing antibody AbD09097 recognizes an epitope adjacent to sclerostin's binding site for the Wnt co-receptor LRP6.
Open Biol. 2016 Aug 24: 6:160120. doi: 10.1098/rsob.160120
Steinbach et al., (2016) CD4+ and γδ T Cells are the main Producers of IL-22 and IL-17A in Lymphocytes from Mycobacterium bovis-infected Cattle.
Sci Rep 6, Article number:29990 (2016) doi:10.1038/srep29990.
Golden et al., (2016) A Recombinant Positive Control for Serology Diagnostic Tests Supporting Elimination of Onchocerca volvulus.
PLoS Negl Trop Dis. 2016 Jan 8;10(1):e0004292. doi: 10.1371/journal.pntd.0004292. eCollection 2016.
Haylock et al., (2016) Evaluation of a novel type of imaging probe based on a recombinant bivalent mini-antibody construct for detection of CD44v6-expressing squamous cell carcinoma.
Int J Oncol. 2016 Feb;48(2):461-70. doi: 10.3892/ijo.2015.3290. Epub 2015 Dec 14.
Spiegelberg et al., (2015) Molecular imaging of EGFR and CD44v6 for prediction and response monitoring of HSP90 inhibition in an in vivo squamous cell carcinoma model.
Eur J Nucl Med Mol Imaging. Published online 2015 Dec 1
Spiegelberg et al., (2015) A real-time in vitro assay as a potential predictor of in vivo tumor imaging properties
Nuclear Medicine and Biology Published online 2015 Sep 20
Shojima et al., (2015) Wnt5a promotes cancer cell invasion and proliferation by receptor-mediated endocytosis-dependent and -independent mechanisms, respectively.
Sci Rep. 2015 Jan 27;5:8042. doi: 10.1038/srep08042.
Boschert et al., (2015) Crystallization and preliminary X-ray crystallographic analysis of the sclerostin-neutralizing Fab AbD09097.
Acta Crystallogr F Struct Biol Commun. 2015 Apr;71(Pt 4):388-92. Epub 2015 Mar 20.
Elgersma et al., (2015) Design, Synthesis, and Evaluation of Linker-Duocarmycin Payloads: Toward Selection of HER2-Targeting Antibody-Drug Conjugate SYD985.
Mol Pharm. 2015 Feb 18. [Epub ahead of print]
Bromage et al. (2014) Characterising a novel antibody for full-length (active) stromal derived factor-1α (1-67).
Heart 2014;100:A13 doi:10.1136/heartjnl-2014-306916.39
Than et al., (2014) Evolutionary origins of the placental expression of chromosome 19 cluster galectins and their complex dysregulation in preeclampsia.
Placenta. 2014 Nov;35(11):855-65. doi: 10.1016/j.placenta.2014.07.015. [Epub 2014 Aug 26.]
Whiteaker et al., (2014) High-affinity recombinant antibody fragments (Fab) can be applied in peptide enrichment immuno-MRM assays.
J Proteome Res. 2014 Feb 25. [Epub ahead of print]
Colakoglu, G. et al. (2013) Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system.
Proc Natl Acad Sci USA 2014 Jan 2 [Epub ahead of print]
Gustchina, E. et al. (2013) Complexes of neutralizing and non-neutralizing affinity matured Fabs with a mimetic of the internal trimeric coiled-coil of HIV-1 gp41.
PLoS ONE 8(11): e78187. doi:10.1371/journal.pone.0078187
Ylera, F. et al. (2013) Off-rate screening for selection of high-affinity anti-drug antibodies.
Anal Biochem. Jul 29 doi: 10.1016/j.ab.2013.07.025 [Epub ahead of print]
Whelan, S.F.J. et al (2013) Distinct characteristics of antibody responses against factor VIII in healthy individuals and in different cohorts of hemophilia A patients.
Blood Feb 7 doi: 10.1182/blood-2012-07-444877 [Epub 2012 Dec 12]
Van Dinther, M. et al. (2013) Anti-Sclerostin antibody inhibits internalization of Sclerostin and Sclerostin-mediated antagonism of Wnt/LRP6 signaling.
PLoS One Apr 29 doi: 10.1371/journal.pone.0062295. [Print 2013]
Nilvebrant, J. et al. (2012) Selection and in vitro characterization of human CD44v6-binding antibody fragments.
Biotech & App Biochem. Sep 20 [Epub ahead of print]
Matsuda, Y. et al. (2012) Fibroblast growth factor receptor 2 IIIc as a therapeutic target for colorectal cancer cells.
Mol Cancer Ther. Jul 9. [Epub ahead of print]
Gregory, A.P. et al. (2012) TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis.
Nature. 2012 Jul 8. doi:10.1038/nature11307. [Epub ahead of print]
Casrouge, A. et al. (2012) Discrimination of agonist and antagonist forms of CXCL10 in biological samples.
Clin Exp Immunol. 167:137-48
Back, J.W. et al. (2012) Selecting highly structure-specific antibodies using structured synthetic mimics of the cystine knot protein sclerostin.
Protein Engineering, Design & Selection 25: 251–9
Petrul, H.M. et al. (2012) Therapeutic mechanism and efficacy of the antibody-drug conjugate BAY 79-4620 targeting human carbonic anhydrase 9.
Mol Cancer Ther. 2012 Feb;11(2):340-9 [Epub ahead of print]
Ostrowitzki, S. et al. (2012) Mechanism of amyloid removal in patients with Alzheimer disease treated with Gantenerumab.
Arch Neurol. 2011 Oct 10 [Epub ahead of print]
Bohrmann, B. et al. (2012) Gantenerumab: A novel human anti-Aβ antibody demonstrates sustained cerebral amyloid-β binding and elicits cell-mediated removal of human amyloid-β
J Alzheimers Dis. 2011 Sep 28 [Epub ahead of print]
Shapiro, R.I. et al. (2011) Development and validation of immunoassays to quantify the half-antibody exchange of an IgG4 antibody, natalizumab (Tysabri®) with endogenous IgG4.
J Pharm Biomed Anal. 55:168-75
Whelan, O.W. et al. (2011) Development of an antibody to bovine IL-2 reveals multifunctional CD4 TEM cells in cattle naturally infected with bovine tuberculosis.
PLoS One. 2011 Dec 22 e29194 [Epub ahead of print]
Giblin, P. et al. (2011) Fully human antibodies against the protease-activated receptor-2 (PAR-2) with anti-inflammatory activity.
Hum Antibodies. 20:83-94
Prassler, J. et al. (2011) HuCAL PLATINUM, a synthetic Fab library optimized for sequence diversity and superior performance in mammalian expression systems.
J. Mol Biol. 413:261-78
Schwaeble, W.J. et al. (2011) Targeting of mannan-binding lectin-associated serine protease-2 confers protection from myocardial and gastrointestinal ischemia/reperfusion injury.
Proc Natl Acad Sci U S A. 108:7523-8
Kwong, L.S. et al. (2011) Characterisation of antibodies to bovine Toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2.
Vet Immunol Immunopathol. 139:313-8
Casrouge, A. et al. (2011) Evidence for an antagonist form of the chemokine CXCL10 in patients chronically infected with HCV.
J Clin Invest. 121:308-17
Rankl, C. et al. (2010) Detection of corneodesmosin on the surface of stratum corneum using atomic force microscopy.
Exp Dermatol. 19:1014-1019
Gustchina, E. et al. (2010) Structural Basis of HIV-1 Neutralization by Affinity Matured Fabs Directed against the Internal Trimeric Coiled-Coil of gp41.
PLoS Pathog. 6:e1001182
Ogawa, S. et al. (2010) Anti-PDGF-B monoclonal antibody reduces liver fibrosis development.
Hepatology Res. 40:1128–1141
Harth, S. et al. (2010) A Selection Fit Mechanism in BMP Receptor IA as a Possible Source for BMP Ligand-Receptor Promiscuity.
PLoS One. 28;5(9). pii: e13049
Larkin, E. et al. (2010) Inhibition of Toxic Shock by Human Monoclonal Antibodies against Staphylococcal Enterotoxin B.
PLoS One. 5(10):e13253
Harth, S. et al. (2010) Crystallization of BMP receptor type IA bound to the antibody Fab fragment AbD1556.
Acta Cryst. F66:964–968
Moore, C. (2010) Using recombinant monoclonal antibodies to develop in vitro diagnostics. IVD Technology.
IVD Technology 16:25-32
Shiryaev, S. A. et al. (2010) Isolation and characterization of selective and potent human Fab inhibitors directed to the active-site region of the two-component NS2B-NS3 proteinase of West Nile virus.
Biochem J. 427:369-76
Summer, A. et al. (2010) Short Communication: Characterization of a monoclonal antibody for K-Casein B of cow’s milk.
J Dairy Sci. 93:796-800
Okerlund, N. D. et al. (2010) Dact1 Is a postsynaptic protein required for dendrite, spine, and excitatory synapse development in the mouse forebrain
J Neurosci. 30:4362-8
Ohashi, M., Eagle, R. A., and Trowsdale, J. (2010) Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a GPI-linked isoform.
J Biol Chem. Mar 19. Epub ahead of print
Vallon, M. et al. (2010) Tumor endothelial marker 5 expression in endothelial cells during capillary morphogenesis is induced by the small GTPase Rac and mediates contact inhibition of cell proliferation.
Exp Cell Res. 316:412-421
Bottos, A. et al. (2009) The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions.
Proc Natl Acad Sci U S A. Nov 19. [Epub ahead of print]
Prassler, J. et al. (2009) In vitro affinity maturation of HuCAL antibodies: complementarity determining region exchange and RapMAT technology.
Future Medicine, Immunotherapy. 1:574-583
Gustchina, E. et al. (2009) Affinity maturation by targeted diversification of the CDR-H2 loop of a monoclonal Fab derived from a synthetic naïve human antibody library and directed against the internal trimeric coiled-coil of gp41 yields a set of Fabs with improved HIV-1 neutralization potency and breadth.
Knappik, A. et al. (2009) Development of recombinant human IgA for anticardiolipin antibodies assay standardization.
Ann N Y Acad Sci. 1173:190-198.
Knappik, A. and Brundiers, R. (2009) Recombinant antibody expression and purification. In: Walker, J.M. editor. The Protein Protocols Handbook. 3rd edition.
New York: Humana Press Inc.
Teplyakov, A. et al. (2009) Epitope mapping of anti-interleukin-13 neutralizing antibody CNTO607.
J Mol Biol. 389:115-23.
Jung, S.M., Tsuji, K., and Moroi, M. (2009) Glycoprotein (GP) VI dimer as a major collagen-binding site of native platelets: direct evidence obtained with dimeric GPVI-specific Fabs.
J Thromb Haemost. 7:1347–1355.
Lochmatter, C., Schifferli, J.A., and Martin, P.J. (2009) Schistosoma mansoni TOR is a tetraspanning orphan receptor on the parasite surface.
Ulinger, S. et al. (2008) The path to Platinum: The evolution of the Human Combinatorial Libraries (HuCAL ®), 19th Annual International Conference on Antibody Engineering, 2008 December 7-11. San Diego.
Steidl, S. et al. (2008) In vitro affinity maturation of human GM-CSF antibodies by targeted CDR-diversification.
Mol Immunol. 46:135-44
Larkin, E.A. et al. (2008) Human monoclonal antibodies against staphylococcal enterotoxin B: potential therapeutics.
Abstracts of the General Meeting of the American Society for Microbiology; 2008 June 1-5; Boston, Massachusetts.
Chernov, A.V. et al. (2008) The two-component NS2B-NS3 proteinase represses DNA unwinding activity of the West Nile virus NS3 helicase.
J Biol Chem. 283:17270-17278.
Rothe, C. et al. (2008) The human combinatorial antibody library HuCAL GOLD combines diversification of all six CDRs according to the natural immune system with a novel display method for efficient selection of high-affinity antibodies.
J Mol Biol. 376:1182-1200.
Hillig, R.C. et al. (2008) Fab MOR03268 triggers absorption shift of a diagnostic dye via packaging in a solvent-shielded Fab dimer interface.
J Mol Biol. 377:206-219.
Tornetta, M. (2007) Isolation of human anti-idiotypic antibodies by phage display for clinical immune response assays.
J Immunol Methods. 328:34-44
Barnard, G. et al. (2007) Direct detection of disease-associated prions in brain and lymphoid tissue using antibodies recognizing the extreme N terminus of PrPc.
Gustchina, E. et al. (2007) A monoclonal Fab derived from a human nonimmune phage library reveals a new epitope on gp41 and neutralizes diverse human immunodeficiency virus type 1 strains.
J Virol. 23:12946-12953.
Jarutat, T. et al. (2007) Selection of vimentin-specific antibodies from the HuCAL phage display library by subtractive panning on formalin-fixed, paraffin-embedded tissue.
Biol. Chem. 388: 651-658.
Kolly, R. et al. (2007) Monovalent antibody scFv fragments selected to modulate T-cell activation by inhibition of CD86-CD28 interaction.
Protein Eng Des Sel. 20:91-98.
Koch, H. et al. (2006) Direct selection of antibodies from complex libraries with the protein fragment complementation assay.
J Mol Biol. 357:427-441.
Zimmerman, T. et al. (2006) Simultaneous metal chelate affinity purification and endotoxin clearance of recombinant antibody fragments.
J Immunol Methods. 314:67-73.
Vanhoutteghem, A. and Djian, P. (2006) Basonuclins 1 and 2, whose genes share a common origin, are proteins with widely different properties and functions.
Proc Natl Acad Sci USA. 103:12423-12428.
Jarutat, T. et al. (2006) Isolation and comparative characterization of Ki-67 equivalent antibodies from the HuCAL phage display library.
Biol Chem. 387:995-1003.
Ooe, H., Iguchi-Ariga, S.M., and Ariga, H. (2006) Establishment of specific antibodies that recognize C106-oxidized DJ-1.
Neurosci Lett. 404:166-169.
Ohara, R. et al. (2006) Antibodies for proteomic research: comparison of traditional immunization with recombinant antibody technology.
Boehncke , W.H. et al. (2005) Efficacy of the fully human monoclonal antibody MOR102 (#5) against intercellular adhesion molecule 1 in the psoriasis-severe combined immunodeficient mouse model.
J Dermatol. 153:758-766.
Poetz, O. et al. (2005) Protein microarrays for antibody profiling: specificity and affinity determination on a chip.
Haenel, C. et al. (2005) Characterization of high-affinity antibodies by electrochemiluminescence-based equilibrium titration.
Anal Biochem. 339:182-184.
Louis, J.M. et al. (2005) Characterization and HIV-1 fusion inhibitory properties of monoclonal Fabs obtained from a human non-immune phage library selected against diverse epitopes of the ectodomain of HIV-1 gp41.
J Mol Biol. 353:945-951.
Parsons, C.J. et al. (2004) Antifibrotic effects of a tissue inhibitor of metalloproteinase-1 antibody on established liver fibrosis in rats.
Röthlisberger, D., Pos, K.M., and Pluckthun, A. (2004) An antibody library for stabilizing and crystallizing membrane proteins - selecting binders to the citrate carrier CitS.
FEBS Lett. 564:340-348.
Sun, C. et al. (2003) Kirrel2, a novel immunoglobulin superfamily gene expressed primarily in beta cells of the pancreatic islets.
Rauchenberger, R. et al. (2003) Human combinatorial Fab library yielding specific and functional antibodies against the human fibroblast growth factor receptor 3.
J Biol Chem. 278:38194-38205.
Cesaro-Tadic, S. et al. (2003) Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library.
Nat Biotechnol. 21:679-685.
Ewert, S. et al. (2003) Biophysical properties of human antibody variable domains.
J Mol Biol. 325:531-553.
Ewert, S., Honegger, A., and Plückthun, A. (2003) Structure-based improvement of the biophysical properties of immunoglobulin VH domains with a generalizable approach.
Frisch, C. et al. (2003) From EST to IHC: human antibody pipeline for target research.
J. Immunol. Methods 275:203-212.
Pavlickova, P. et al. (2003) Microarray of recombinant antibodies using a streptavidin sensor surface self-assembled onto a gold layer.
Kretzschmar, T. and von Rüden, T. (2002) Antibody discovery: phage display.
Curr Opin Biotechnol. 13:598-602.
Blank, K. et al. (2002) Self-immobilizing recombinant antibody fragments for immunoaffinity chromatography: generic, parallel, and scalable protein purification.
Protein Expr Purif. 24:313-322.
Nagy, Z.A. et al. (2002) Fully human, HLA-DR-specific monoclonal antibodies efficiently induce programmed death of malignant lymphoid cells.
Nat Med. 8:801-807.
Krebs, B. et al. (2001) High-throughput generation and engineering of recombinant human antibodies.
J Immunol Methods. 254:67-84.
Marget, M., et al. (2000) Bypassing hybridoma technology: HLA-C reactive hman single-chain antibody fragments (scFv) derived from a synthetic phage display library (HuCAL) and their potential to discriminate HLA class I specificities.
Tissue Antigens. 56:1-9.
Hanes, J. et al. (2000) Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display.
Nat Biotechnol. 18:1287-92.
Frisch, C. et al. (2000) Titration of infective and noninfective Ff filamentous bacteriophages using a monoclonal antibody against g3p.
Knappik, A. et al. (2000) Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides.
J Mol Biol. 296:57-86.
Plückthun, A. and Pack, P (1997) New protein engineering approaches to multivalent and bispecific antibody fragments.
Rheinnecker, M. et al. (1996) Multivalent antibody fragments with high functional affinity for a tumor-associated carbohydrate antigen.
J Immunol. 157:2989-2997.
Knappik, A. and Plückthun, A. (1995) Engineered turns of a recombinant antibody improve its in vivo folding.
Protein Eng. 8:81-90.
Virnekäs, B. et al. (1994) Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.
Nucleic Acids Res. 22:5600-5607.
Pack, P. et al. (1993) Improved bivalent miniantibodies, with identical avidity as whole antibodies, produced by high cell density fermentation of Escherichia coli.
Biotechnology (NY). 11:1271-1277.