HuCAL References


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Publications citing the use of HuCAL® antibodies and the HuCAL technology.

Abed K et al. (2023) One-year longitudinal study of the stratum corneum proteome of retinol and all-trans-retinoic acid treated human skin: an orchestrated molecular event. 
Sci Rep 13(1):11196 

Alalwany RH et al. (2023). Vascular endothelial growth factor isoforms differentially protect neurons against neurotoxic events associated with Alzheimer's disease. 
Front Mol Neurosci 16:1181626 

Holder A et al. (2023). Characterisation of the bovine C-type lectin receptor Mincle and potential evidence for an endogenous ligand. 
Front Immunol 14:1189587 

Longarini EJ et al. (2023). Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling. 
Mol Cell 83(10):1743-1760.e11 

Dowall SD et al. (2023). Development of humanised antibodies for Crimean-Congo Haemorrhagic fever virus: Comparison of hybridoma-based versus phage library techniques. 
J Immunol Methods 512:113405 

Malhi NK et al. (2022). Serine-arginine-rich protein kinase-1 inhibition for the treatment of diabetic retinopathy. 
Am J Physiol Heart Circ Physiol 322(6):H1014-H1027 

Thompson S et al. (2023). Nitration of chemokine CXCL8 acts as a natural mechanism to limit acute inflammation.
Cell Mol Life Sci 23, 80

Yang X et al. (2022). Exploring the value of Mycobacterium tuberculosis modified lipoprotein as a potential biomarker for TB detection in children.
BMC Infect Dis 22, 158

Marcus J et al. (2021). PARP-1 activation leads to cytosolic accumulation of TDP-43 in neurons.
Neurochem Int 148, 105077

Bonfiglio J et al. (2020). An HPF1/PARP1-Based Chemical Biology Strategy for Exploring ADP-Ribosylation
Cell 183 (4) 1086-1102

Chatterjee T et al. (2020). Direct kinetic fingerprinting and digital counting of single protein molecules
PNAS 117 (37) 22815-22822

Magyarics Z et al. (2019). Randomized, Double-Blind, Placebo-Controlled, Single-Ascending-Dose Study of the Penetration of a Monoclonal Antibody Combination (ASN100) Targeting Staphylococcus aureus Cytotoxins in the Lung Epithelial Lining Fluid of Healthy Volunteers
Antimicrobial Agents and Chemotherapy 63(8) e00350-19

van Baak M et al. (2019). Adipose tissue contribution to plasma fibroblast growth factor 21 and fibroblast activation protein in obesity.
Int J Obes (Lond). 2019 Aug 27 [Epub ahead of print]

Dvela-Levitt M et al. (2019). Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy
Cell 178(3), 521-535

Edwards A et al. (2018). Adaptation of the human Cell Line Activation Test (h-CLAT) to Animal-Product-Free Conditions 
ALTEX 35(4), 477-488

Harth S et al. (2019). Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics
mAbs 11:178-90

Clark J et al. (2018). Cardioprotection by an anti-MASP-2 antibody in a murine model of myocardial infarction
Open Heart 5 Epub e000652

Morvan et al. (2017). Blockade of activin type II receptors with a dual anti-ActRIIA/IIB antibody is critical to promote maximal skeletal muscle hypertrophy
Proc Natl Acad Sci U S A. [Epub ahead of print]

Holbrook L et al. (2017). A humanized monoclonal antibody that inhibits platelet-surface ERp72 reveals a role for ERp72 in thrombosis.
J Thromb Haemost [Epub ahead of print]

Marri L et al. (2017). An enzyme-linked immunosorbent assay for the detection of diacetyl (2,3-butanedione)
Anal Biochem 535:12-18

Shepherd D et al. (2017). The Subventricular Zone Response to Stroke Is Not a Therapeutic Target of Anti-Nogo-A Immunotherapy.
J Neuropathol Exp Neurol. 76(8):683-696

Xu E et al. (2017). SOX4 Allows Facultative ß-Cell Proliferation Through Repression of Cdkn1a
Diabetes 66: 2213-2219

Bernasconi-Elias P et al. (2016). Characterization of activating mutations of NOTCH3 in T-cell acute lymphoblastic leukemia and anti-leukemic activity of NOTCH3 inhibitory antibodies.
Oncogene 35:6077-6086

Chen Z et al. (2016). Discovery and Characterization of Phage Display-Derived Human Monoclonal Antibodies against RSV F Glycoprotein.
PLoS One 11(6):e0156798

Bromageet et al. (2017). A novel recombinant antibody specific to full-length stromal derived factor-1 for potential application in biomarker studies.
PLoS ONE 2017 April 5: 12(4): e0174447 doi: 10.1371/journet al.pone.0174447

?Boschert et al. (2016). The sclerostin-neutralizing antibody AbD09097 recognizes an epitope adjacent to sclerostin's binding site for the Wnt co-receptor LRP6. 
Open Biol. 2016 Aug 24: 6:160120. doi: 10.1098/rsob.160120

Steinbach et al. (2016). CD4+ and γδ T Cells are the main Producers of IL-22 and IL-17A in Lymphocytes from Mycobacterium bovis-infected Cattle.
Sci Rep 6, Article number:29990 (2016). doi:10.1038/srep29990.

Golden et al. (2016). A Recombinant Positive Control for Serology Diagnostic Tests Supporting Elimination of Onchocerca volvulus.
PLoS Negl Trop Dis. 2016 Jan 8;10(1):e0004292. doi: 10.1371/journet al.pntd.0004292. eCollection 2016.

Haylock et al. (2016). Evaluation of a novel type of imaging probe based on a recombinant bivalent mini-antibody construct for detection of CD44v6-expressing squamous cell carcinoma.
Int J Oncol. 2016 Feb;48(2):461-70. doi: 10.3892/ijo.2015.3290. Epub 2015 Dec 14.

Spiegelberg et al. (2015). Molecular imaging of EGF R and CD44v6 for prediction and response monitoring of HSP90 inhibition in an in vivo squamous cell carcinoma model.
Eur J Nucl Med Mol Imaging. Published online 2015 Dec 1

Spiegelberg et al. (2015). A real-time in vitro assay as a potential predictor of in vivo tumor imaging properties
Nuclear Medicine and Biology Published online 2015 Sep 20

Shojima et al. (2015). Wnt5a promotes cancer cell invasion and proliferation by receptor-mediated endocytosis-dependent and -independent mechanisms, respectively.
Sci Rep. 2015 Jan 27;5:8042. doi: 10.1038/srep08042.

Boschert et al. (2015). Crystallization and preliminary X-ray crystallographic analysis of the sclerostin-neutralizing Fab AbD09097.
Acta Crystallogr F Struct Biol Commun. 2015 Apr;71(Pt 4):388-92. Epub 2015 Mar 20.

Elgersma et al. (2015). Design, Synthesis, and Evaluation of Linker-Duocarmycin Payloads: Toward Selection of HER2-Targeting Antibody-Drug Conjugate SYD985.
Mol Pharm. 2015 Feb 18. [Epub ahead of print]

Bromageet et al. (2014). Characterising a novel antibody for full-length (active) stromal derived factor-1α (1-67).
Heart 2014;100:A13 doi:10.1136/heartjnl-2014-306916.39

Thanet et al. (2014). Evolutionary origins of the placental expression of chromosome 19 cluster galectins and their complex dysregulation in preeclampsia.
Placenta. 2014 Nov;35(11):855-65. doi: 10.1016/j.placenta.2014.07.015. [Epub 2014 Aug 26.]

Whiteaker et al. (2014). High-affinity recombinant antibody fragments (Fab) can be applied in peptide enrichment immuno-MRM assays.
J Proteome Res. 2014 Feb 25. [Epub ahead of print]

Colakoglu, al. (2013). Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system.
Proc Natl Acad Sci USA 2014 Jan 2 [Epub ahead of print]

Gustchina, E. et al. (2013). Complexes of neutralizing and non-neutralizing affinity matured Fabs with a mimetic of the internal trimeric coiled-coil of HIV-1 gp41.
PLoS ONE 8(11): e78187. doi:10.1371/journet al.pone.0078187

Ylera, F. et al. (2013). Off-rate screening for selection of high-affinity anti-drug antibodies.
Anal Biochem. Jul 29 doi: 10.1016/j.ab.2013.07.025 [Epub ahead of print]

Whelan, S.F.J. et al. (2013). Distinct characteristics of antibody responses against factor VIII in healthy individuals and in different cohorts of hemophilia A patients.
Blood Feb 7 doi: 10.1182/blood-2012-07-444877 [Epub 2012 Dec 12]

Van Dinther, M. et al. (2013). Anti-Sclerostin antibody inhibits internalization of Sclerostin and Sclerostin-mediated antagonism of Wnt/LRP6 signaling.
PLoS One Apr 29 doi: 10.1371/journet al.pone.0062295. [Print 2013]

Nilvebrant, al. (2012). Selection and in vitro characterization of human CD44v6-binding antibody fragments.
Biotech & App Biochem. Sep 20  [Epub ahead of print]

Matsuda, Y.  et al. (2012). Fibroblast growth factor receptor 2 IIIc as a therapeutic target for colorectal cancer cells.
Mol Cancer Ther.  Jul 9.  [Epub ahead of print]

Gregory,  A.P. et al. (2012). TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis.
Nature. 2012 Jul 8. doi:10.1038/nature11307. [Epub ahead of print]

Casrouge, A. et al. (2012). Discrimination of agonist and antagonist forms of CXCL10 in biological samples.
Clin Exp Immunol. 167:137-48

Back, J.W. et al. (2012). Selecting highly structure-specific antibodies using structured synthetic mimics of the cystine knot protein sclerostin.
Protein Engineering, Design & Selection 25: 251–9

Petrul, H.M. et al. (2012). Therapeutic mechanism and efficacy of the antibody-drug conjugate BAY 79-4620 targeting human carbonic anhydrase 9.
Mol Cancer Ther. 2012 Feb;11(2):340-9 [Epub ahead of print]

Ostrowitzki, S. et al. (2012). Mechanism of amyloid removal in patients with Alzheimer disease treated with Gantenerumab.
Arch Neurol. 2011 Oct 10 [Epub ahead of print]

Bohrmann, B. et al. (2012). Gantenerumab: A novel human anti-Aβ antibody demonstrates sustained cerebral amyloid-β binding and elicits cell-mediated removal of human amyloid-β
J Alzheimers Dis. 2011 Sep 28 [Epub ahead of print]

Shapiro, R.I. et al. (2011). Development and validation of immunoassays to quantify the half-antibody exchange of an IgG4 antibody, natalizumab (Tysabri®) with endogenous IgG4.
J Pharm Biomed Anet al.55:168-75

Whelan, O.W. et al. (2011). Development of an antibody to bovine IL-2 reveals multifunctional CD4 TEM cells in cattle naturally infected with bovine tuberculosis.
PLoS One. 2011 Dec 22 e29194 [Epub ahead of print]

Giblin, P. et al. (2011). Fully human antibodies against the protease-activated receptor-2 (PAR-2) with anti-inflammatory activity.
Hum Antibodies. 20:83-94

Prassler, J. et al. (2011). HuCAL PLATINUM, a synthetic Fab library optimized for sequence diversity and superior performance in mammalian expression systems.
J. Mol Biol. 413:261-78

Schwaeble, W.J.  et al. (2011). Targeting of mannan-binding lectin-associated serine protease-2 confers protection from myocardial and gastrointestinal ischemia/reperfusion injury.
Proc Natl Acad Sci U S A. 108:7523-8

Kwong, L.S. et al. (2011). Characterisation of antibodies to bovine Toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2.
Vet Immunol Immunopathol. 139:313-8

Casrouge, A. et al. (2011). Evidence for an antagonist form of the chemokine CXCL10 in patients chronically infected with HCV.
J Clin Invest. 121:308-17

Rankl, C. et al. (2010). Detection of corneodesmosin on the surface of stratum corneum using atomic force microscopy.
Exp Dermatol. 19:1014-1019

Gustchina, E. et al. (2010). Structural Basis of HIV-1 Neutralization by Affinity Matured Fabs Directed against the Internal Trimeric Coiled-Coil of gp41.
PLoS Pathog. 6:e1001182

Ogawa, S. et al. (2010). Anti-PDGF-B monoclonal antibody reduces liver fibrosis development.
Hepatology Res. 40:1128–1141

Harth, S. et al. (2010). A Selection Fit Mechanism in BMP Receptor IA as a Possible Source for BMP Ligand-Receptor Promiscuity.
PLoS One. 28;5(9). pii: e13049

Larkin, E. et al. (2010). Inhibition of Toxic Shock by Human Monoclonal Antibodies against Staphylococcal Enterotoxin B.
PLoS One. 5(10):e13253

Harth, S. et al. (2010). Crystallization of BMP receptor type IA bound to the antibody Fab fragment AbD1556.
Acta Cryst. F66:964–968

Shiryaev, S. A.  et al. (2010). Isolation and characterization of selective and potent human Fab inhibitors directed to the active-site region of the two-component NS2B-NS3 proteinase of West Nile virus.
Biochem J. 427:369-76

Summer, A. et al. (2010). Short Communication: Characterization of a monoclonal antibody for K-Casein B of cow’s milk.
J Dairy Sci. 93:796-800

Okerlund, N. D. et al. (2010). Dact1 Is a postsynaptic protein required for dendrite, spine, and excitatory synapse development in the mouse forebrain
J Neurosci. 30:4362-8

Ohashi, M., Eagle, R. A., and Trowsdale, J. (2010). Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a GPI-linked isoform.
J Biol Chem. Mar 19. Epub ahead of print

Vallon, M. et al. (2010). Tumor endothelial marker 5 expression in endothelial cells during capillary morphogenesis is induced by the small GTPase Rac and mediates contact inhibition of cell proliferation.
Exp Cell Res. 316:412-421

Bottos, A. et al. (2009). The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions.
Proc Natl Acad Sci U S A. Nov 19. [Epub ahead of print]

Prassler, J. et al. (2009). In vitro affinity maturation of HuCAL antibodies: complementarity determining region exchange and RapMAT technology.
Future Medicine, Immunotherapy. 1:574-583

Gustchina, E. et al. (2009). Affinity maturation by targeted diversification of the CDR-H2 loop of a monoclonal Fab derived from a synthetic naïve human antibody library and directed against the internal trimeric coiled-coil of gp41 yields a set of Fabs with improved HIV-1 neutralization potency and breadth.
Virology. 39:112-119.

Knappik, A. et al. (2009). Development of recombinant human IgA for anticardiolipin antibodies assay standardization.
Ann N Y Acad Sci. 1173:190-198.

Knappik, A. and Brundiers, R. (2009). Recombinant antibody expression and purification. In: Walker, J.M. editor. The Protein Protocols Handbook. 3rd edition.
New York: Humana Press Inc.

Teplyakov, A. et al. (2009). Epitope mapping of anti-interleukin-13 neutralizing antibody CNTO607.
J Mol Biol. 389:115-23.

Jung, S.M., Tsuji, K., and Moroi, M. (2009). Glycoprotein (GP) VI dimer as a major collagen-binding site of native platelets: direct evidence obtained with dimeric GPVI-specific Fabs.
J Thromb Haemost. 7:1347–1355.

Lochmatter, C., Schifferli, J.A., and Martin, P.J. (2009). Schistosoma mansoni TOR is a tetraspanning orphan receptor on the parasite surface.
Parasitology. 136:487-498.

Ulinger, S. et al. (2008). The path to Platinum: The evolution of the Human Combinatorial Libraries (HuCAL ®), 19th Annual International Conference on Antibody Engineering, 2008 December 7-11. San Diego.

Steidl, S. et al. (2008). In vitro affinity maturation of human GM-CSF antibodies by targeted CDR-diversification.
Mol Immunol. 46:135-44

Larkin, E.A. et al. (2008). Human monoclonal antibodies against staphylococcal enterotoxin B: potential therapeutics.
Abstracts of the General Meeting of the American Society for Microbiology; 2008 June 1-5; Boston, Massachusetts.

Chernov, A.V. et al. (2008). The two-component NS2B-NS3 proteinase represses DNA unwinding activity of the West Nile virus NS3 helicase.
J Biol Chem. 283:17270-17278.

Rothe, C. et al. (2008). The human combinatorial antibody library HuCAL GOLD combines diversification of all six CDRs according to the natural immune system with a novel display method for efficient selection of high-affinity antibodies.
J Mol Biol. 376:1182-1200.

Hillig, R.C. et al. (2008). Fab MOR03268 triggers absorption shift of a diagnostic dye via packaging in a solvent-shielded Fab dimer interface.
J Mol Biol. 377:206-219.

Tornetta, M. (2007). Isolation of human anti-idiotypic antibodies by phage display for clinical immune response assays.
J Immunol Methods. 328:34-44

Barnard, G. et al. (2007). Direct detection of disease-associated prions in brain and lymphoid tissue using antibodies recognizing the extreme N terminus of PrPc.
Prion. 1:121-127.

Gustchina, E. et al. (2007). A monoclonal Fab derived from a human nonimmune phage library reveals a new epitope on gp41 and neutralizes diverse human immunodeficiency virus type 1 strains.
J Virol. 23:12946-12953.

Jarutat, T. et al. (2007). Selection of vimentin-specific antibodies from the HuCAL phage display library by subtractive panning on formalin-fixed, paraffin-embedded tissue.
Biol. Chem. 388: 651-658.

Kolly, R. et al. (2007). Monovalent antibody scFv fragments selected to modulate T-cell activation by inhibition of CD86-CD28 interaction.
Protein Eng Des Sel. 20:91-98.

Koch, H. et al. (2006). Direct selection of antibodies from complex libraries with the protein fragment complementation assay.
J Mol Biol. 357:427-441.

Zimmerman, T. et al. (2006). Simultaneous metal chelate affinity purification and endotoxin clearance of recombinant antibody fragments.
J Immunol Methods. 314:67-73.

Vanhoutteghem, A. and Djian, P. (2006). Basonuclins 1 and 2, whose genes share a common origin, are proteins with widely different properties and functions.
Proc Natl Acad Sci USA. 103:12423-12428.

Jarutat, T. et al. (2006). Isolation and comparative characterization of Ki-67 equivalent antibodies from the HuCAL phage display library.
Biol Chem. 387:995-1003.

Ooe, H., Iguchi-Ariga, S.M., and Ariga, H. (2006). Establishment of specific antibodies that recognize C106-oxidized DJ-1.
Neurosci Lett. 404:166-169.

Ohara, R. et al. (2006). Antibodies for proteomic research: comparison of traditional immunization with recombinant antibody technology.
Proteomics. 6:2638-2646.

Boehncke , W.H. et al. (2005). Efficacy of the fully human monoclonal antibody MOR102 (#5) against intercellular adhesion molecule 1 in the psoriasis-severe combined immunodeficient mouse model.
J Dermatol. 153:758-766.

Poetz, O. et al. (2005). Protein microarrays for antibody profiling: specificity and affinity determination on a chip.
Proteomics. 5:2402-2411.

Haenel, C. et al. (2005). Characterization of high-affinity antibodies by electrochemiluminescence-based equilibrium titration.
Anal Biochem. 339:182-184.

Louis, J.M. et al. (2005). Characterization and HIV-1 fusion inhibitory properties of monoclonal Fabs obtained from a human non-immune phage library selected against diverse epitopes of the ectodomain of HIV-1 gp41.
J Mol Biol. 353:945-951.

Parsons, C.J. et al. (2004). Antifibrotic effects of a tissue inhibitor of metalloproteinase-1 antibody on established liver fibrosis in rats.
Hepatology. 40:1106-1115.

Röthlisberger, D., Pos, K.M., and Pluckthun, A. (2004). An antibody library for stabilizing and crystallizing membrane proteins - selecting binders to the citrate carrier CitS.
FEBS Lett. 564:340-348.

Sun, C. et al. (2003). Kirrel2, a novel immunoglobulin superfamily gene expressed primarily in beta cells of the pancreatic islets.
Genomics 82:130-142.

Rauchenberger, R. et al. (2003). Human combinatorial Fab library yielding specific and functional antibodies against the human fibroblast growth factor receptor 3.
J Biol Chem. 278:38194-38205.

Cesaro-Tadic, S. et al. (2003). Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library.
Nat Biotechnol. 21:679-685.

Ewert, S. et al. (2003). Biophysical properties of human antibody variable domains.
J Mol Biol. 325:531-553.

Ewert, S., Honegger, A., and Plückthun, A. (2003). Structure-based improvement of the biophysical properties of immunoglobulin VH domains with a generalizable approach.
Biochemistry. 42:1517-1528.

Frisch, C. et al. (2003). From EST to IHC: human antibody pipeline for target research.
J. Immunol. Methods 275:203-212.

Pavlickova, P. et al. (2003). Microarray of recombinant antibodies using a streptavidin sensor surface self-assembled onto a gold layer.
Biotechniques. 34:124-130.

Kretzschmar, T. and von Rüden, T. (2002). Antibody discovery: phage display.
Curr Opin Biotechnol. 13:598-602.

Blank, K. et al. (2002). Self-immobilizing recombinant antibody fragments for immunoaffinity chromatography: generic, parallel, and scalable protein purification.
Protein Expr Purif. 24:313-322.

Nagy, Z.A. et al. (2002). Fully human, HLA-DR-specific monoclonal antibodies efficiently induce programmed death of malignant lymphoid cells.
Nat Med. 8:801-807.

Krebs, B. et al. (2001). High-throughput generation and engineering of recombinant human antibodies.
J Immunol Methods. 254:67-84.

Marget, M., et al. (2000). Bypassing hybridoma technology: HLA-C reactive hman single-chain antibody fragments (scFv) derived from a synthetic phage display library (HuCAL) and their potential to discriminate HLA class I specificities.
Tissue Antigens. 56:1-9.

Hanes, J. et al. (2000). Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display.
Nat Biotechnol. 18:1287-92.

Frisch, C. et al. (2000). Titration of infective and noninfective Ff filamentous bacteriophages using a monoclonal antibody against g3p.
Biotechniques. 29:26-30.

Knappik, A. et al. (2000). Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides.
J Mol Biol. 296:57-86.

Plückthun, A. and Pack, P (1997). New protein engineering approaches to multivalent and bispecific antibody fragments.
Immunotechnology, 3:83-105.

Rheinnecker, M. et al. (1996). Multivalent antibody fragments with high functional affinity for a tumor-associated carbohydrate antigen.
J Immunol. 157:2989-2997.

Knappik, A. and Plückthun, A. (1995). Engineered turns of a recombinant antibody improve its in vivo folding.
Protein Eng. 8:81-90.

Virnekäs, B. et al. (1994).Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.
Nucleic Acids Res. 22:5600-5607.

Pack, P. et al. (1993). Improved bivalent miniantibodies, with identical avidity as whole antibodies, produced by high cell density fermentation of Escherichia coli.
Biotechnology (NY). 11:1271-1277.