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Antibodies Designed Just for You

HuCAL® Recombinant Monoclonal Antibody Generation Service


Publications citing the use of HuCAL® antibodies and the HuCAL technology.

Clark J et al., (2018) Cardioprotection by an anti-MASP-2 antibody in a murine model of myocardial infarction
Open Heart 5 Epub e000652


Morvan et al., (2017) Blockade of activin type II receptors with a dual anti-ActRIIA/IIB antibody is critical to promote maximal skeletal muscle hypertrophy
Proc Natl Acad Sci U S A. [Epub ahead of print]


Holbrook L et al., (2017) A humanized monoclonal antibody that inhibits platelet-surface ERp72 reveals a role for ERp72 in thrombosis.
J Thromb Haemost [Epub ahead of print]


Marri L et al., (2017) An enzyme-linked immunosorbent assay for the detection of diacetyl (2,3-butanedione)
Anal Biochem 535:12-18


Shepherd D et al., (2017) The Subventricular Zone Response to Stroke Is Not a Therapeutic Target of Anti-Nogo-A Immunotherapy.
J Neuropathol Exp Neurol. 76(8):683-696


Xu E et al., (2017) SOX4 Allows Facultative ß-Cell Proliferation Through Repression of Cdkn1a
Diabetes 66: 2213-2219


Bernasconi-Elias P et al., (2016) Characterization of activating mutations of NOTCH3 in T-cell acute lymphoblastic leukemia and anti-leukemic activity of NOTCH3 inhibitory antibodies.
Oncogene 35:6077-6086


Chen Z et al., (2016) Discovery and Characterization of Phage Display-Derived Human Monoclonal Antibodies against RSV F Glycoprotein.
PLoS One 11(6):e0156798


Bromage et al., (2017) A novel recombinant antibody specific to full-length stromal derived factor-1 for potential application in biomarker studies.
PLoS ONE 2017 April 5: 12(4): e0174447 doi: 10.1371/journal.pone.0174447


Boschert et al., (2016) The sclerostin-neutralizing antibody AbD09097 recognizes an epitope adjacent to sclerostin's binding site for the Wnt co-receptor LRP6. 
Open Biol. 2016 Aug 24: 6:160120. doi: 10.1098/rsob.160120


Steinbach et al., (2016) CD4+ and γδ T Cells are the main Producers of IL-22 and IL-17A in Lymphocytes from Mycobacterium bovis-infected Cattle.
Sci Rep 6, Article number:29990 (2016) doi:10.1038/srep29990.


Golden et al., (2016) A Recombinant Positive Control for Serology Diagnostic Tests Supporting Elimination of Onchocerca volvulus.
PLoS Negl Trop Dis. 2016 Jan 8;10(1):e0004292. doi: 10.1371/journal.pntd.0004292. eCollection 2016.


Haylock et al., (2016) Evaluation of a novel type of imaging probe based on a recombinant bivalent mini-antibody construct for detection of CD44v6-expressing squamous cell carcinoma.
Int J Oncol. 2016 Feb;48(2):461-70. doi: 10.3892/ijo.2015.3290. Epub 2015 Dec 14.


Spiegelberg et al., (2015) Molecular imaging of EGF R and CD44v6 for prediction and response monitoring of HSP90 inhibition in an in vivo squamous cell carcinoma model.
Eur J Nucl Med Mol Imaging. Published online 2015 Dec 1


Spiegelberg et al., (2015) A real-time in vitro assay as a potential predictor of in vivo tumor imaging properties
Nuclear Medicine and Biology Published online 2015 Sep 20


Shojima et al., (2015) Wnt5a promotes cancer cell invasion and proliferation by receptor-mediated endocytosis-dependent and -independent mechanisms, respectively.
Sci Rep. 2015 Jan 27;5:8042. doi: 10.1038/srep08042.


Boschert et al., (2015) Crystallization and preliminary X-ray crystallographic analysis of the sclerostin-neutralizing Fab AbD09097.
Acta Crystallogr F Struct Biol Commun. 2015 Apr;71(Pt 4):388-92. Epub 2015 Mar 20.


Elgersma et al., (2015) Design, Synthesis, and Evaluation of Linker-Duocarmycin Payloads: Toward Selection of HER2-Targeting Antibody-Drug Conjugate SYD985.
Mol Pharm. 2015 Feb 18. [Epub ahead of print]


Bromage et al. (2014) Characterising a novel antibody for full-length (active) stromal derived factor-1α (1-67).
Heart 2014;100:A13 doi:10.1136/heartjnl-2014-306916.39


Than et al., (2014) Evolutionary origins of the placental expression of chromosome 19 cluster galectins and their complex dysregulation in preeclampsia.
Placenta. 2014 Nov;35(11):855-65. doi: 10.1016/j.placenta.2014.07.015. [Epub 2014 Aug 26.]


Whiteaker et al., (2014) High-affinity recombinant antibody fragments (Fab) can be applied in peptide enrichment immuno-MRM assays.
J Proteome Res. 2014 Feb 25. [Epub ahead of print]


Colakoglu, G. et al. (2013) Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system.
Proc Natl Acad Sci USA 2014 Jan 2 [Epub ahead of print]


Gustchina, E. et al. (2013) Complexes of neutralizing and non-neutralizing affinity matured Fabs with a mimetic of the internal trimeric coiled-coil of HIV-1 gp41.
PLoS ONE 8(11): e78187. doi:10.1371/journal.pone.0078187


Ylera, F. et al. (2013) Off-rate screening for selection of high-affinity anti-drug antibodies.
Anal Biochem. Jul 29 doi: 10.1016/j.ab.2013.07.025 [Epub ahead of print]


Whelan, S.F.J. et al (2013) Distinct characteristics of antibody responses against factor VIII in healthy individuals and in different cohorts of hemophilia A patients.
Blood Feb 7 doi: 10.1182/blood-2012-07-444877 [Epub 2012 Dec 12]


Van Dinther, M. et al. (2013) Anti-Sclerostin antibody inhibits internalization of Sclerostin and Sclerostin-mediated antagonism of Wnt/LRP6 signaling.
PLoS One Apr 29 doi: 10.1371/journal.pone.0062295. [Print 2013]


Nilvebrant, J. et al.  (2012) Selection and in vitro characterization of human CD44v6-binding antibody fragments.
Biotech & App Biochem. Sep 20  [Epub ahead of print]


Matsuda, Y.  et al.  (2012) Fibroblast growth factor receptor 2 IIIc as a therapeutic target for colorectal cancer cells.
Mol Cancer Ther.  Jul 9.  [Epub ahead of print]


Gregory,  A.P. et al. (2012) TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis.
Nature. 2012 Jul 8. doi:10.1038/nature11307. [Epub ahead of print]


Casrouge, A. et al. (2012) Discrimination of agonist and antagonist forms of CXCL10 in biological samples.
Clin Exp Immunol. 167:137-48


Back, J.W.  et al. (2012) Selecting highly structure-specific antibodies using structured synthetic mimics of the cystine knot protein sclerostin.
Protein Engineering, Design & Selection 25: 251–9


Petrul, H.M. et al. (2012) Therapeutic mechanism and efficacy of the antibody-drug conjugate BAY 79-4620 targeting human carbonic anhydrase 9.
Mol Cancer Ther. 2012 Feb;11(2):340-9 [Epub ahead of print]


Ostrowitzki, S. et al. (2012) Mechanism of amyloid removal in patients with Alzheimer disease treated with Gantenerumab.
Arch Neurol. 2011 Oct 10 [Epub ahead of print]


Bohrmann, B. et al. (2012) Gantenerumab: A novel human anti-Aβ antibody demonstrates sustained cerebral amyloid-β binding and elicits cell-mediated removal of human amyloid-β
J Alzheimers Dis. 2011 Sep 28 [Epub ahead of print]


Shapiro, R.I. et al. (2011) Development and validation of immunoassays to quantify the half-antibody exchange of an IgG4 antibody, natalizumab (Tysabri®) with endogenous IgG4.
J Pharm Biomed Anal. 55:168-75


Whelan, O.W. et al. (2011) Development of an antibody to bovine IL-2 reveals multifunctional CD4 TEM cells in cattle naturally infected with bovine tuberculosis.
PLoS One. 2011 Dec 22 e29194 [Epub ahead of print]


Giblin, P. et al. (2011) Fully human antibodies against the protease-activated receptor-2 (PAR-2) with anti-inflammatory activity.
Hum Antibodies. 20:83-94


Prassler, J. et al. (2011) HuCAL PLATINUM, a synthetic Fab library optimized for sequence diversity and superior performance in mammalian expression systems.
J. Mol Biol. 413:261-78


Schwaeble, W.J. et al. (2011) Targeting of mannan-binding lectin-associated serine protease-2 confers protection from myocardial and gastrointestinal ischemia/reperfusion injury.
Proc Natl Acad Sci U S A. 108:7523-8


Kwong, L.S. et al. (2011) Characterisation of antibodies to bovine Toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2.
Vet Immunol Immunopathol. 139:313-8


Casrouge, A. et al. (2011) Evidence for an antagonist form of the chemokine CXCL10 in patients chronically infected with HCV.
J Clin Invest. 121:308-17


Rankl, C. et al. (2010) Detection of corneodesmosin on the surface of stratum corneum using atomic force microscopy.
Exp Dermatol. 19:1014-1019


Gustchina, E. et al. (2010) Structural Basis of HIV-1 Neutralization by Affinity Matured Fabs Directed against the Internal Trimeric Coiled-Coil of gp41.
PLoS Pathog. 6:e1001182


Ogawa, S. et al. (2010) Anti-PDGF-B monoclonal antibody reduces liver fibrosis development.
Hepatology Res. 40:1128–1141


Harth, S. et al. (2010) A Selection Fit Mechanism in BMP Receptor IA as a Possible Source for BMP Ligand-Receptor Promiscuity.
PLoS One. 28;5(9). pii: e13049


Larkin, E. et al. (2010) Inhibition of Toxic Shock by Human Monoclonal Antibodies against Staphylococcal Enterotoxin B.
PLoS One. 5(10):e13253


Harth, S. et al. (2010) Crystallization of BMP receptor type IA bound to the antibody Fab fragment AbD1556.
Acta Cryst. F66:964–968


Moore, C. (2010) Using recombinant monoclonal antibodies to develop in vitro diagnostics. IVD Technology.
IVD Technology 16:25-32


Shiryaev, S. A. et al. (2010) Isolation and characterization of selective and potent human Fab inhibitors directed to the active-site region of the two-component NS2B-NS3 proteinase of West Nile virus.
Biochem J. 427:369-76


Summer, A. et al. (2010) Short Communication: Characterization of a monoclonal antibody for K-Casein B of cow’s milk.
J Dairy Sci. 93:796-800


Okerlund, N. D. et al. (2010) Dact1 Is a postsynaptic protein required for dendrite, spine, and excitatory synapse development in the mouse forebrain
J Neurosci. 30:4362-8


Ohashi, M., Eagle, R. A., and Trowsdale, J. (2010) Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a GPI-linked isoform.
J Biol Chem. Mar 19. Epub ahead of print


Vallon, M. et al. (2010) Tumor endothelial marker 5 expression in endothelial cells during capillary morphogenesis is induced by the small GTPase Rac and mediates contact inhibition of cell proliferation.
Exp Cell Res. 316:412-421


Bottos, A. et al. (2009) The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions.
Proc Natl Acad Sci U S A. Nov 19. [Epub ahead of print]


Prassler, J. et al. (2009) In vitro affinity maturation of HuCAL antibodies: complementarity determining region exchange and RapMAT technology.
Future Medicine, Immunotherapy. 1:574-583


Gustchina, E. et al. (2009) Affinity maturation by targeted diversification of the CDR-H2 loop of a monoclonal Fab derived from a synthetic naïve human antibody library and directed against the internal trimeric coiled-coil of gp41 yields a set of Fabs with improved HIV-1 neutralization potency and breadth.
Virology. 39:112-119.


Knappik, A. et al. (2009) Development of recombinant human IgA for anticardiolipin antibodies assay standardization.
Ann N Y Acad Sci. 1173:190-198.


Knappik, A. and Brundiers, R. (2009) Recombinant antibody expression and purification. In: Walker, J.M. editor. The Protein Protocols Handbook. 3rd edition.
New York: Humana Press Inc.


Teplyakov, A. et al. (2009) Epitope mapping of anti-interleukin-13 neutralizing antibody CNTO607.
J Mol Biol. 389:115-23.


Jung, S.M., Tsuji, K., and Moroi, M. (2009) Glycoprotein (GP) VI dimer as a major collagen-binding site of native platelets: direct evidence obtained with dimeric GPVI-specific Fabs.
J Thromb Haemost. 7:1347–1355.


Lochmatter, C., Schifferli, J.A., and Martin, P.J. (2009) Schistosoma mansoni TOR is a tetraspanning orphan receptor on the parasite surface.
Parasitology. 136:487-498.


Ulinger, S. et al. (2008) The path to Platinum: The evolution of the Human Combinatorial Libraries (HuCAL ®), 19th Annual International Conference on Antibody Engineering, 2008 December 7-11. San Diego.
Presentation


Steidl, S. et al. (2008) In vitro affinity maturation of human GM-CSF antibodies by targeted CDR-diversification.
Mol Immunol. 46:135-44


Larkin, E.A. et al. (2008) Human monoclonal antibodies against staphylococcal enterotoxin B: potential therapeutics.
Abstracts of the General Meeting of the American Society for Microbiology; 2008 June 1-5; Boston, Massachusetts.


Chernov, A.V. et al. (2008) The two-component NS2B-NS3 proteinase represses DNA unwinding activity of the West Nile virus NS3 helicase.
J Biol Chem. 283:17270-17278.


Rothe, C. et al. (2008) The human combinatorial antibody library HuCAL GOLD combines diversification of all six CDRs according to the natural immune system with a novel display method for efficient selection of high-affinity antibodies.
J Mol Biol. 376:1182-1200.


Hillig, R.C. et al. (2008) Fab MOR03268 triggers absorption shift of a diagnostic dye via packaging in a solvent-shielded Fab dimer interface.
J Mol Biol. 377:206-219.


Tornetta, M. (2007) Isolation of human anti-idiotypic antibodies by phage display for clinical immune response assays.
J Immunol Methods. 328:34-44


Barnard, G. et al. (2007) Direct detection of disease-associated prions in brain and lymphoid tissue using antibodies recognizing the extreme N terminus of PrPc.
Prion. 1:121-127.


Gustchina, E. et al. (2007) A monoclonal Fab derived from a human nonimmune phage library reveals a new epitope on gp41 and neutralizes diverse human immunodeficiency virus type 1 strains.
J Virol. 23:12946-12953.


Jarutat, T. et al. (2007) Selection of vimentin-specific antibodies from the HuCAL phage display library by subtractive panning on formalin-fixed, paraffin-embedded tissue.
Biol. Chem. 388: 651-658.


Kolly, R. et al. (2007) Monovalent antibody scFv fragments selected to modulate T-cell activation by inhibition of CD86-CD28 interaction.
Protein Eng Des Sel. 20:91-98.


Koch, H. et al. (2006) Direct selection of antibodies from complex libraries with the protein fragment complementation assay.
J Mol Biol. 357:427-441.


Zimmerman, T. et al. (2006) Simultaneous metal chelate affinity purification and endotoxin clearance of recombinant antibody fragments.
J Immunol Methods. 314:67-73.


Vanhoutteghem, A. and Djian, P. (2006) Basonuclins 1 and 2, whose genes share a common origin, are proteins with widely different properties and functions.
Proc Natl Acad Sci USA. 103:12423-12428.


Jarutat, T. et al. (2006) Isolation and comparative characterization of Ki-67 equivalent antibodies from the HuCAL phage display library.
Biol Chem. 387:995-1003.


Ooe, H., Iguchi-Ariga, S.M., and Ariga, H. (2006) Establishment of specific antibodies that recognize C106-oxidized DJ-1.
Neurosci Lett. 404:166-169.


Ohara, R. et al. (2006) Antibodies for proteomic research: comparison of traditional immunization with recombinant antibody technology.
Proteomics. 6:2638-2646.


Boehncke , W.H. et al. (2005) Efficacy of the fully human monoclonal antibody MOR102 (#5) against intercellular adhesion molecule 1 in the psoriasis-severe combined immunodeficient mouse model.
J Dermatol. 153:758-766.


Poetz, O. et al. (2005) Protein microarrays for antibody profiling: specificity and affinity determination on a chip.
Proteomics. 5:2402-2411.


Haenel, C. et al. (2005) Characterization of high-affinity antibodies by electrochemiluminescence-based equilibrium titration.
Anal Biochem. 339:182-184.


Louis, J.M. et al. (2005) Characterization and HIV-1 fusion inhibitory properties of monoclonal Fabs obtained from a human non-immune phage library selected against diverse epitopes of the ectodomain of HIV-1 gp41.
J Mol Biol. 353:945-951.


Parsons, C.J. et al. (2004) Antifibrotic effects of a tissue inhibitor of metalloproteinase-1 antibody on established liver fibrosis in rats.
Hepatology. 40:1106-1115.


Röthlisberger, D., Pos, K.M., and Pluckthun, A. (2004) An antibody library for stabilizing and crystallizing membrane proteins - selecting binders to the citrate carrier CitS.
FEBS Lett. 564:340-348.


Sun, C. et al. (2003) Kirrel2, a novel immunoglobulin superfamily gene expressed primarily in beta cells of the pancreatic islets.
Genomics 82:130-142.


Rauchenberger, R. et al. (2003) Human combinatorial Fab library yielding specific and functional antibodies against the human fibroblast growth factor receptor 3.
J Biol Chem. 278:38194-38205.


Cesaro-Tadic, S. et al. (2003) Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library.
Nat Biotechnol. 21:679-685.


Ewert, S. et al. (2003) Biophysical properties of human antibody variable domains.
J Mol Biol. 325:531-553.


Ewert, S., Honegger, A., and Plückthun, A. (2003) Structure-based improvement of the biophysical properties of immunoglobulin VH domains with a generalizable approach.
Biochemistry. 42:1517-1528.


Frisch, C. et al. (2003) From EST to IHC: human antibody pipeline for target research.
J. Immunol. Methods 275:203-212.


Pavlickova, P. et al. (2003) Microarray of recombinant antibodies using a streptavidin sensor surface self-assembled onto a gold layer.
Biotechniques. 34:124-130.


Kretzschmar, T. and von Rüden, T. (2002) Antibody discovery: phage display.
Curr Opin Biotechnol. 13:598-602.


Blank, K. et al. (2002) Self-immobilizing recombinant antibody fragments for immunoaffinity chromatography: generic, parallel, and scalable protein purification.
Protein Expr Purif. 24:313-322.


Nagy, Z.A. et al. (2002) Fully human, HLA-DR-specific monoclonal antibodies efficiently induce programmed death of malignant lymphoid cells.
Nat Med. 8:801-807.


Krebs, B. et al. (2001) High-throughput generation and engineering of recombinant human antibodies.
J Immunol Methods. 254:67-84.


Marget, M., et al. (2000) Bypassing hybridoma technology: HLA-C reactive hman single-chain antibody fragments (scFv) derived from a synthetic phage display library (HuCAL) and their potential to discriminate HLA class I specificities.
Tissue Antigens. 56:1-9.


Hanes, J. et al. (2000) Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display.
Nat Biotechnol. 18:1287-92.


Frisch, C. et al. (2000) Titration of infective and noninfective Ff filamentous bacteriophages using a monoclonal antibody against g3p.
Biotechniques. 29:26-30.


Knappik, A. et al. (2000) Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides.
J Mol Biol. 296:57-86.


Plückthun, A. and Pack, P (1997) New protein engineering approaches to multivalent and bispecific antibody fragments.
Immunotechnology, 3:83-105.


Rheinnecker, M. et al. (1996) Multivalent antibody fragments with high functional affinity for a tumor-associated carbohydrate antigen.
J Immunol. 157:2989-2997.


Knappik, A. and Plückthun, A. (1995) Engineered turns of a recombinant antibody improve its in vivo folding.
Protein Eng. 8:81-90.


Virnekäs, B. et al. (1994) Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.
Nucleic Acids Res. 22:5600-5607.


Pack, P. et al. (1993) Improved bivalent miniantibodies, with identical avidity as whole antibodies, produced by high cell density fermentation of Escherichia coli.
Biotechnology (NY). 11:1271-1277.