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Normal Goat serum used as a blocking agent to minimize non-specific staining in sections stained for tyrosine hydroxylase and caspase using immunofluorescence.
A proportion of murine TH-IR cells undergo apoptotic changes on postnatal day P2, as identified by immunostaining for activated caspase-3 (a), or by the presence of nuclear condensation (b), white arrow an example of pronounced nuclear condensation. TH-IR cell apoptosis was quantified in the combined SNc and VTA at postnatal day P2 in male and female wild-type (c) or annexin A1 null (d) mice treated with dexamethasone via the maternal drinking water antenatally on embryonic days 16–19 (grey bars 0.5 µg/ml), and compared to the control offspring of dams receiving normal drinking water (open bars). Data are mean ± SEM, n = 6 animals per treatment group. Asterisk a significant effect of treatment, p < 0.05 for dexamethasone-treated vs. control animals
From: McArthur S, Pienaar IS, Siddiqi SM, Gillies GE. Sex-specific disruption of murine midbrain astrocytic and dopaminergic developmental trajectories following antenatal GC treatment. Brain Struct Funct. 2015 May 6. [Epub ahead of print].