Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts
Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts
Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents
There are many considerations for sample preparation to optimize your flow cytometry. The first one is to take into account your starting sample. Frozen cells will need to be treated differently to an adherent cell line for example; however there are some basic rules that can be followed for most samples that will help you optimize your experiments.