FITC Antibody | LO-FLUO-1

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  • Rat anti FITC:Biotin
  • Rat anti FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    LO-FLUO-1
  • Isotype
    IgM
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1332BE, Fdatasheet pdfdatasheet pdf0.5 mg
    MCA1332B
    MCA1332E, Fdatasheet pdfdatasheet pdf0.5 mg
    MCA1332
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti FITC antibody, clone LO-FLUO-1 reacts with fluorescein isothiocyanate. Avidity against FITC conjugated BSA is 2 x 1010M-1.

      This antibody binds to both Free FITC and FITC conjugated to proteins, and may be valuable for amplification of FITC based immunostaining.
    • Intended Use
    • Target Species
      Chemical
    • Product Form
      Purified IgM conjugated to Biotin - liquid
      Purified IgM - liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by affinity chromatography from tissue culture supernatant
      Purified IgM prepared by affinity chromatography from tissue culture supernatant
    • Preservative Stabilisers
      50%Glycerol
      0.1%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      FITC conjugated mouse immunoglobulin.
    • Purity
    • Approx. Protein Concentrations
      Isotype concentration 1.0 mg/ml
      Isotype concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5ug/ml
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1.25ug/ml2.25ug/ml
      Flow Cytometry

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional FITC Antibody Formats

    Formats Clone Applications Sizes available
    FITC Antibody : Biotin LO-FLUO-1 E, F 0.5 mg
    FITC Antibody : Purified LO-FLUO-1 E, F 0.5 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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