ELISA IgM Reducing Assay Diluent

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  • ELISA IgM Reducing Assay Diluent
  • ELISA IgM Reducing Assay Diluent
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF038AEdatasheet pdfdatasheet pdf100 ml
    BUF038A
    BUF038BEdatasheet pdfdatasheet pdf500 ml
    BUF038B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
      Pack Size: 100 mlBUF038A is an ELISA assay diluent designed to reduce background noise and assist in matrix equalization in problematic serum and plasma samples, especially those affected by high levels of “sticky” IgM which can non-specifically bind the HRP conjugate. Specially formulated for use in sandwich ELISA, it contains mammalian protein additives based on normal goat serum to reduce non-specific interactions between sample proteins and the plate surface.

      The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. BUF038A equalizes the matrix, reduces IgM-mediated bridging interference, inhibits complement and thrombin activity and contains an antimicrobial agent.
      Pack Size: 500 mlBUF038B is an ELISA assay diluent designed to reduce background noise and assist in matrix equalization in problematic serum and plasma samples, especially those affected by high levels of “sticky” IgM which can non-specifically bind the HRP conjugate. Specially formulated for use in sandwich ELISA, it contains mammalian protein additives based on normal goat serum to reduce non-specific interactions between sample proteins and the plate surface.

      The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. BUF038B equalizes the matrix, reduces IgM-mediated bridging interference, inhibits complement and thrombin activity and contains an antimicrobial agent.
      Pack Size: 100 mlBUF038A is an ELISA assay diluent designed to reduce background noise and assist in matrix equalization in problematic serum and plasma samples, especially those affected by high levels of “sticky” IgM which can non-specifically bind the HRP conjugate. Specially formulated for use in sandwich ELISA, it contains mammalian protein additives based on normal goat serum to reduce non-specific interactions between sample proteins and the plate surface.

      The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. BUF038A equalizes the matrix, reduces IgM-mediated bridging interference, inhibits complement and thrombin activity and contains an antimicrobial agent.
      Pack Size: 500 mlBUF038B is an ELISA assay diluent designed to reduce background noise and assist in matrix equalization in problematic serum and plasma samples, especially those affected by high levels of “sticky” IgM which can non-specifically bind the HRP conjugate. Specially formulated for use in sandwich ELISA, it contains mammalian protein additives based on normal goat serum to reduce non-specific interactions between sample proteins and the plate surface.

      The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. BUF038B equalizes the matrix, reduces IgM-mediated bridging interference, inhibits complement and thrombin activity and contains an antimicrobial agent.
    • Product Form
      Ready to use - liquid
      Ready to use - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.10% Sodium Azide
      1-5% Sodium Metabisulphite
      0.10% Sodium Azide
      1-5% Sodium Metabisulphite
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC.

      DO NOT FREEZE
      Store at +4oC.

      DO NOT FREEZE
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISANeat
    • Application NameYesNoMin DilutionMax Dilution
      ELISANeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      Pipette buffer directly onto the plate, just prior to adding the sample or standard (suggested volume 50 - 100ul/well).

      NB. Since this buffer contains proteins derived from goat, BUF038A is not recommended for ELISA where an anti goat secondary antibody is used.
    • Instructions For Use
      Pipette buffer directly onto the plate, just prior to adding the sample or standard (suggested volume 50 - 100ul/well).

      NB. Since this buffer contains proteins derived from goat, BUF038B is not recommended for ELISA where an anti goat secondary antibody is used.

    Additional ELISA IgM Reducing Assay Diluent Formats

    Formats Applications Sizes available
    ELISA IgM Reducing Assay Diluent : Ready To Use E 100 ml | 500 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          5x ELISA Coating BufferBUF030A100 mlE
          BUF030A
          10x ELISA Wash BufferBUF031A100 mlE
          BUF031A
          ELISA BSA BlockBUF032A100 mlE
          BUF032A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          5x ELISA Coating BufferBUF030A100 mlE
          BUF030A
          10x ELISA Wash BufferBUF031A100 mlE
          BUF031A
          ELISA BSA BlockBUF032A100 mlE
          BUF032A

          Recommended Positive Controls

            Histology Controls

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