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ELISA BSA Block
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Enlarge- ELISA BSA Block
- ELISA BSA Block
- ELISA BSA Block
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Product TypeAccessory Reagent
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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Intended UsePack Size: 1000 mlBUF032C is a general purpose ELISA blocking buffer for use in all ELISA formats requiring low to average blocking strength. The buffer contains a mammalian protein blocking agent based on BSA, proprietary protein stabilizers and an antimicrobial agent. It provides a long-term stable environment for coating antigen or capture antibody, whilst minimizing non-specific binding interactions in the assay process. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.Pack Size: 500 mlBUF032B is a general purpose ELISA blocking buffer for use in all ELISA formats requiring low to average blocking strength. The buffer contains a mammalian protein blocking agent based on BSA, proprietary protein stabilizers and an antimicrobial agent. It provides a long-term stable environment for coating antigen or capture antibody, whilst minimizing non-specific binding interactions in the assay process. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.Pack Size: 100 mlBUF032A is a general purpose ELISA blocking buffer for use in all ELISA formats requiring low to average blocking strength. The buffer contains a mammalian protein blocking agent based on BSA, proprietary protein stabilizers and an antimicrobial agent. It provides a long-term stable environment for coating antigen or capture antibody, whilst minimizing non-specific binding interactions in the assay process. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.Pack Size: 1000 mlBUF032C is a general purpose ELISA blocking buffer for use in all ELISA formats requiring low to average blocking strength. The buffer contains a mammalian protein blocking agent based on BSA, proprietary protein stabilizers and an antimicrobial agent. It provides a long-term stable environment for coating antigen or capture antibody, whilst minimizing non-specific binding interactions in the assay process. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.Pack Size: 500 mlBUF032B is a general purpose ELISA blocking buffer for use in all ELISA formats requiring low to average blocking strength. The buffer contains a mammalian protein blocking agent based on BSA, proprietary protein stabilizers and an antimicrobial agent. It provides a long-term stable environment for coating antigen or capture antibody, whilst minimizing non-specific binding interactions in the assay process. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.Pack Size: 100 mlBUF032A is a general purpose ELISA blocking buffer for use in all ELISA formats requiring low to average blocking strength. The buffer contains a mammalian protein blocking agent based on BSA, proprietary protein stabilizers and an antimicrobial agent. It provides a long-term stable environment for coating antigen or capture antibody, whilst minimizing non-specific binding interactions in the assay process. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.Pack Size: 1000 mlBUF032C is a general purpose ELISA blocking buffer for use in all ELISA formats requiring low to average blocking strength. The buffer contains a mammalian protein blocking agent based on BSA, proprietary protein stabilizers and an antimicrobial agent. It provides a long-term stable environment for coating antigen or capture antibody, whilst minimizing non-specific binding interactions in the assay process. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.Pack Size: 500 mlBUF032B is a general purpose ELISA blocking buffer for use in all ELISA formats requiring low to average blocking strength. The buffer contains a mammalian protein blocking agent based on BSA, proprietary protein stabilizers and an antimicrobial agent. It provides a long-term stable environment for coating antigen or capture antibody, whilst minimizing non-specific binding interactions in the assay process. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.Pack Size: 100 mlBUF032A is a general purpose ELISA blocking buffer for use in all ELISA formats requiring low to average blocking strength. The buffer contains a mammalian protein blocking agent based on BSA, proprietary protein stabilizers and an antimicrobial agent. It provides a long-term stable environment for coating antigen or capture antibody, whilst minimizing non-specific binding interactions in the assay process. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.
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Product FormReady to use - liquidReady to use - liquidReady to use - liquid
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Reconstitution
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Preparation
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Preservative Stabilisers
0.05% Sodium Azide 0.05% Sodium Azide 0.05% Sodium Azide -
Purity
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Approx. Protein Concentrations
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered salinePhosphate buffered salinePhosphate buffered saline
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StorageStore at +4oC.
DO NOT FREEZE
This product should be stored undiluted.Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted.Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. -
Shelf Life18 months from date of despatch.18 months from date of despatch.Please see label for expiry date.
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA Neat Application Name Yes No Min Dilution Max Dilution ELISA Neat Application Name Yes No Min Dilution Max Dilution ELISA Neat - Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own systems using the appropriate negative/positive controls.
- Technical Advice
- Technical Advice
- Technical Advice
- Recommended Protocol
- Recommended Protocol
- Recommended Protocol
- ELISA
- ELISA
- ELISA
- Immunohistology
- Immunohistology
- Immunohistology
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Immunofluorescence
- Immunofluorescence
- Immunofluorescence
- Western Blotting
- Western Blotting
- Western Blotting
- Instructions For Use1. Coat ELISA plate with antibody or antigen as required.
2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.
3. Add 300-400ul of BUF032C and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.
4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C. - Instructions For Use1. Coat ELISA plate with antibody or antigen as required.
2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.
3. Add 300-400ul of BUF032B and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.
4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C. - Instructions For Use1. Coat ELISA plate with antibody or antigen as required.
2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.
3. Add 300-400ul of BUF032A and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.
4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.
Additional ELISA BSA Block Formats
| Formats | Applications | Sizes available |
|---|---|---|
| ELISA BSA Block : Ready To Use | E | 500 ml | 1000 ml | 100 ml |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Negative Isotype Control
Useful Reagents
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| 5x ELISA Coating Buffer | BUF030A | 100 ml | E | BUF030A | |
| 10x ELISA Wash Buffer | BUF031A | 100 ml | E | BUF031A |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| 5x ELISA Coating Buffer | BUF030A | 100 ml | E | BUF030A | |
| 10x ELISA Wash Buffer | BUF031A | 100 ml | E | BUF031A |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| 5x ELISA Coating Buffer | BUF030A | 100 ml | E | BUF030A | |
| 10x ELISA Wash Buffer | BUF031A | 100 ml | E | BUF031A |
Recommended Positive Controls
Histology Controls
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Formerly known as AbD Serotec, a global supplier and manufacturer of antibodies, kits, and reagents.
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
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