CD4 Antibody | Du CD4-2

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CD4 Antibody | Du CD4-2 gallery image 1

Published customer image:
T lymphocytes in PBLs following DPV gC DNA vaccination. 3, 5, 7, 14, 28, 42 days after vaccination, the isolated PBLs were stained with monoclonal antibodies against duck CD4 (A), and CD8 (B). The results presented are the mean of all specimens of each group ± SD.

From: Lian B, Cheng A, Wang M, Zhu D, Luo Q, Jia R, Liu F, Han X, Chen X. Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C. Virol J. 2011 May 10;8:214.

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CD4 Antibody | Du CD4-2 gallery image 2

Published customer image:T lymphocytes in PBL following DEV UL24 DNA vaccination. 1, 2, 3, 4, 6, 8weeks after vaccination, the isolated PBL were stained with monoclonal antibodies against duck CD4 (A) and CD8 (B). The results presented are the mean of all specimens of each group. Statistical significance was determined using a student’st-test.

From: Yu X, Jia R, Huang J, Shu B, Zhu D, Liu Q, Gao X, Lin M, Yin Z, Wang M, Chen S, Wang Y, Chen X, Cheng A. Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge. Vet Res. 2012 Jul 6;43:56.

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Published customer image:
Mouse antiDuck CD4 antibody, clone Du CD4-2 used for the identification of T-Helper cells in goose organs by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
The location of the AIV H9N2 antigen and goose CD4 and CD8α molecules in several tissues at 5 dpi. The protein locations in the different tissues from AIV H9N2-infected birds were detected by IHC assay (Negative control group: a–g). Viral positive signals and cells positive for CD4 or CD8α antigen appeared dark brown using immunohistochemical staining. SP: SP (A,H,O), BF: BF (B,I,P), SI: SI (C,J,Q), R: R (D,K,R), LU: lung (E,L,S), TR: TR (F,M,T), LI: LI (G,N,U).

From: Zhou H, Chen S, Yan B, Chen H, Wang M, Jia R, Zhu D, Liu M, Liu F, Yang Q, Wu Y, Sun K, Chen X, Jing B, Cheng A.
LPAIV H9N2 Drives the Differential Expression of Goose Interferons and Proinflammatory Cytokines in Both In Vitro and In Vivo Studies. Front Microbiol. 2016 Feb 17;7:166.

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CD4 Antibody | Du CD4-2 gallery image 4

Published customer image:
Mouse anti Duck CD4 antibody, clone Du CD4-2 (MCA2478) used along with Mouse anti Duck CD8&aplha; antibody, clone Du CD8-1 (MCA2479) for the demonstration of lymphocyte subsets in goose spleen sections by immunohistochemistry using formalin fixed, paraffin embedded tissue sections.
Image caption:
Global characteristics of goslings after infection with GPV and H9N2. (A) Body weight change of GPV- and H9N2-infected goslings from day 0 to 5. ** p≤0.01, *** p≤0.001; (B) the expression level of IL-1β and IL-6 in the spleen tissues of GPV- and H9N2-infected goslings. ** p≤0.01, *** p≤0.001; (C) histological changes in goose spleens infected with GPV and H9N2 at 5 dpi. The spleen tissues from (a) mock-, (b) GPV-, and (c) H9N2-infected goslings were stained with H &E, and diffuse hemorrhage was observed in either of virus-infected spleen sections, scale bar = 100 μm; and (D) Detection of (a) GPV and (b) H9N2 antigen, as well as (c,d) CD4- and (e,f) CD8α-positive cells by immunohistochemical analysis. The dark brown represents positive signs for viral antigen, CD4, or CD8α molecules, scale bar = 100 μm.

From: Chen S, Wang A, Sun L, Liu F, Wang M, Jia R, Zhu D, Liu M, Yang Q, Wu Y, Sun K, Chen X, Cheng A.
Immune-Related Gene Expression Patterns in GPV- or H9N2-Infected Goose Spleens.
Int J Mol Sci. 2016 Dec 1;17(12). pii: E1990.

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  • Mouse anti Duck CD4
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    Du CD4-2
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2478F, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA2478
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Duck CD4 antibody, clone Du CD4-2 recognizes Pekin duck CD4, shown to be expressed by thymocytes, splenocytes and peripheral lymphoid cells.

      Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs, the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA), and all cells reacting with clone Du CD4-2 have been identified as CD3+ T cells (Kothlow et al. 2005).

      Clone Du CD4-2 can be used to identify duck T helper cells. This antibody has been shown to not react with Mallard.
    • Intended Use
    • Target Species
      Duck
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Chickenno
      Gooseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      293T cells expressing Pekin duck CD4.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry110 ug/ml
      Immunoprecipitation

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD4 Antibody Formats

    Formats Clone Applications Sizes available
    CD4 Antibody : Purified Du CD4-2 F, IP 0.25 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

              Further Reading

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