Presented at AAI 2023, the features of StarBright™ Dyes, are highlighted using data acquired on the ZE5 Cell Analyzer. Additionally, a 38-color human immunophenotyping panel demonstrates that using antibodies conjugated to StarBright Blue and StarBright Yellow Dyes can expand panel size in spectral flow cytometry.
The benefits of using StarBright Dyes for multiplexing are highlighted, such as narrow excitation and emission profiles and premixing.
38-color spectral panel, with a low complexity score, using StarBright Dyes on a 5-laser spectral flow cytometer.
Several posters presented at CYTO 2023 demonstrate the benefits of antibodies conjugated to the StarBright Dye range in several flow cytometry applications. These include large immunophenotyping panels, identifying rare cell populations, and using tetramers to detect antigen-specific T cells.
A 27-color panel on the ZE5 Cell Analyzer demonstrates the use of the StarBright Dye range in a panel without the need for a special buffer. The benefits of using StarBright Yellow Dyes compared to PE-tandem dyes for multiplexing is also highlighted.
StarBright Dyes are ideal for use in spectral flow cytometry as shown in a large 43-color panel with a low complexity score. Unique spectral profiles of StarBright Yellow and StarBright Red Dyes enable the use of novel fluorophore combinations.
StarBright Dyes are bright, stable, and consistent making them a superior choice to detect and characterize rare cell populations, such as hematopoietic stem cells.
New StarBright Red Dyes are ideal for conventional and spectral flow cytometry. In addition, StarBright Dyes bound to tetramers via streptavidin can be used to identify antigen-specific T cells.
Spectral unmixing analysis and autofluorescence removal can improve population resolution over traditional compensation. For example, when studying pHrodo Green phagocytosis in J774 cells in combination with surface staining using StarBright Dye conjugated antibodies.
Presented at CYTO 2022, these 21-color and 31-color, human immunophenotyping panels, showcase how the new StarBright Blue and StarBright Yellow Dyes can be used to expand both dye choice and panel size in immunophenotyping. Useful as a reference when building your own panels, see how these posters featuring StarBright Dyes in both conventional and multicolor flow cytometry. Featuring data obtained using StarBright Dyes and the ZE5 Cell Analyzer and StarBright Dyes and a spectral flow cytometer, respectively.
31-color spectral panel built using StarBright Dyes and a 4-laser spectral flow cytometer.
21-color panel built using only antibodies from Bio-Rad and the ZE5 Cell Analyzer.
Presented at AAI 2022, these human immunophenotyping panels, showcase how StarBright Dyes can be used to expand panel size in multicolor immunophenotyping panels. Useful as a reference when building your own panels, see how these posters featuring StarBright Dyes and the ZE5 Cell Analyzer can help you build bigger better panels.
Poster comparing the stain index of StarBright Dyes to dyes from other vendors, and the effect of pre-mixing antibody cocktails containing StarBright Violet and UltraViolet Dyes compared to freshly made up cocktails.
11-color panels introducing StarBright Blue and StarBright Yellow Dyes on the ZE5 Cell Analyzer.
Presented at CYTO 2021, these 18-color and 27-color, human immunophenotyping panels, generated using StarBright Dyes and the ZE5 Cell Analyzer and a spectral flow cytometer respectively, highlight how to build successful panels while showcasing the properties and benefits of StarBright Dyes in large multicolor panel experiments.
Perfect as a reference when building your own panels.
27-color spectral panel built using StarBright Dyes and a spectral flow cytometer.
18-color panel built using StarBright Dyes and the ZE5 Cell Analyzer.
Presented at AAI 2021, the two posters below highlight the incredible performance of StarBright Violet Dyes in conventional and spectral flow cytometry. These exceptionally bright dyes, with improved spectral properties, have better performance in multicolor panels and require no special buffer, compared to competitor dyes. Furthermore, they are photostable, fixable, and can be premixed for up to 28 days. Their unique spectra allow the use of novel dye combinations.
The benefits of StarBright Dyes are highlighted in conventional and full spectrum flow cytometry.
Comparison of StarBright Dyes in multicolor immunophenotyping without the use of special buffers.