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TNF Alpha

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  • Recombinant Bovine TNF Alpha
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PBP005E, FNdatasheet pdfdatasheet pdf20 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Bovine Tumour Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes. TNF alpha is an important mediator of the bovine inflammatory response.

      PBP005 has an ED50 in the range of 10-25 ng/ml, determined by measuring the cytolytic effect of recombinant bovine TNF alpha on murine WEHI-13VAR cells in the presence of Actinomycin D.
    • Intended Use
    • Target Species
    • Product Form
      Purified Recombinant Protein- lyophilised
    • Reconstitution
      Reconstitute with 0.5 ml distilled water.

      Further dilutions should be made in a buffer containing carrier protein.

      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Recombinant protein expressed in Pichia pastoris
    • Preservative Stabilisers
      None present
    • Purity
      >95% by SDS PAGE analysis
    • Approx. Protein Concentrations
    • Protein Molecular Weight
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      less than 0.04 EU/ug
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      Following reconstitution store at -70oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      cytokine activity
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      PBP005 may be used as a standard with MCA2334 or MCA2335B.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional TNF Alpha Formats

    Formats Applications Sizes available
    TNF Alpha : Rec. Protein E, FN 20 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images


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